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Team:The University of Alberta/16 July 2008
From 2008.igem.org
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*The plates of Transformants of I0500 in the new vector turned out good | *The plates of Transformants of I0500 in the new vector turned out good | ||
**Did colony PCR on two colonies to confirm they have the I0500 insert | **Did colony PCR on two colonies to confirm they have the I0500 insert | ||
| - | ***Streaked out both colonies | + | ***Ran products out on a 1% agarose gel |
| - | + | **Streaked out both colonies | |
| + | **Also made O/Ns of the colonies | ||
*Transformations of the synthed biobricks worked! | *Transformations of the synthed biobricks worked! | ||
**Colony PCR to check the inserts | **Colony PCR to check the inserts | ||
**Set up O/Ns | **Set up O/Ns | ||
Latest revision as of 21:22, 16 July 2008
Today
- The plates of Transformants of I0500 in the new vector turned out good
- Did colony PCR on two colonies to confirm they have the I0500 insert
- Ran products out on a 1% agarose gel
- Streaked out both colonies
- Also made O/Ns of the colonies
- Did colony PCR on two colonies to confirm they have the I0500 insert
- Transformations of the synthed biobricks worked!
- Colony PCR to check the inserts
- Set up O/Ns
