Latest revision as of 21:04, 19 July 2008

Thursday, July 17th

David Ouyang
- Digest miniprep amber suppressor
- ligate in front R0040
- transform!
Taylor Stevenson
Primers for lambda phage genomic modification have finally arrived (NOTE: to view primers download this link and change extension to .doc (word file)[1])
PCR conditions
1. 1uL of template (miniprepped BleoC in pSBA13, and NEB lambda DNA)
2. annealing temp = 55*C
3. extension time = 3min
4. 30 cycles

(1kb standard, BleoC PCR product, Stf PCR product)

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

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+=Thursday, July 17th=
 *David Ouyang
 **Digest miniprep amber suppressor
 **ligate in front R0040
 **transform!
+*Taylor Stevenson
+*Primers for lambda phage genomic modification have finally arrived (NOTE: to view primers download this link and change extension to .doc (word file)[https://static.igem.org/mediawiki/2008/0/0d/Phage_round_1_primers.mov])
+*PCR conditions
+*#1uL of template (miniprepped BleoC in pSBA13, and NEB lambda DNA)
+*#annealing temp = 55*C
+*#extension time = 3min
+*#30 cycles
+[[Image:20080717.jpg|400px]]
+(1kb standard, BleoC PCR product, Stf PCR product)
+<BR><BR><BR><BR>
+{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+!align="center"|[[Team:Rice_University|Home]]
+!align="center"|[[Team:Rice_University/Team|The Team]]
+!align="center"|[[Team:Rice_University/Project|The Project]]
+!align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Rice_University/Modeling|Modeling]]
+!align="center"|[[Team:Rice_University/Notebook|Notebook]]
+|}