Team:University of Ottawa/18 July 2008

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__TOC__
__TOC__
==Today in the Lab==
==Today in the Lab==
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::<li> For miniprep on Monday, I think I will choose 5 colonies per plate.
::<li> For miniprep on Monday, I think I will choose 5 colonies per plate.
::<li> An inoculation will be performed today into liquid media so that a miniprep can be performed.
::<li> An inoculation will be performed today into liquid media so that a miniprep can be performed.
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'''Dan'''
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:'''PCR'''
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::<li> PCR amplification of 0B and 1 A was performed today, absorbance measurements indicated decent concentrations
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:'''Digestion'''
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::<li>1A 0B and T were pooled and digested with PstI and SphI as part of our new strategy for designing the constructs. It should help eliminate virtually all possible byproducts.
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:'''Ligation'''
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::<li> Was performed overnight.
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'''Tammy'''
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:'''Gel Electrophoresis of pDR197::AtCKX2 Digestion Products'''
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::<li> Total plasmid size = 7.9 kb
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::<li> AtCKX2 insert size = 1.67 kb (NM_127508)
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::<li> pDR197 vector only = 6.23 kb
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:'''Glycerol Stock confirmed pDR197::AtCKX2'''
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::<li> Stocked PURE I and 1:10 II
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::<li> 0.5 mL Sample
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::<li> 0.5 ml LB + Glucose

Latest revision as of 20:45, 24 July 2008

Untitled Document

 

 


Contents

Today in the Lab

Matt

PCR Amplification
  • A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be performed next week. Corey suggested it is better to make new stock of this PCR amplification product for integration.
    • Transformation
  • The transformation was a success with both plates streaked growing numerous colonies.
  • For miniprep on Monday, I think I will choose 5 colonies per plate.
  • An inoculation will be performed today into liquid media so that a miniprep can be performed.

    • Dan

    PCR
  • PCR amplification of 0B and 1 A was performed today, absorbance measurements indicated decent concentrations
    • Digestion
  • 1A 0B and T were pooled and digested with PstI and SphI as part of our new strategy for designing the constructs. It should help eliminate virtually all possible byproducts.
    • Ligation
  • Was performed overnight.

    • Tammy

    Gel Electrophoresis of pDR197::AtCKX2 Digestion Products
  • Total plasmid size = 7.9 kb
  • AtCKX2 insert size = 1.67 kb (NM_127508)
  • pDR197 vector only = 6.23 kb
    • Glycerol Stock confirmed pDR197::AtCKX2
  • Stocked PURE I and 1:10 II
  • 0.5 mL Sample
  • 0.5 ml LB + Glucose
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/18_July_2008"