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Team:Rice University/Notebook/17 July 2008
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**transform! | **transform! | ||
*Taylor Stevenson | *Taylor Stevenson | ||
| - | * | + | *Primers for lambda phage genomic modification have finally arrived (NOTE: to view primers download this link and change extension to .doc (word file)[https://static.igem.org/mediawiki/2008/0/0d/Phage_round_1_primers.mov]) |
| + | *PCR conditions | ||
| + | *#1uL of template (miniprepped BleoC in pSBA13, and NEB lambda DNA) | ||
| + | *#annealing temp = 55*C | ||
| + | *#extension time = 3min | ||
| + | *#30 cycles | ||
| + | [[Image:20080717.jpg|400px]] | ||
| + | (1kb standard, BleoC PCR product, Stf PCR product) | ||
| + | <BR><BR><BR><BR> | ||
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
!align="center"|[[Team:Rice_University|Home]] | !align="center"|[[Team:Rice_University|Home]] | ||
Latest revision as of 21:04, 19 July 2008
Thursday, July 17th
- David Ouyang
- Digest miniprep amber suppressor
- ligate in front R0040
- transform!
- Taylor Stevenson
- Primers for lambda phage genomic modification have finally arrived (NOTE: to view primers download this link and change extension to .doc (word file)[1])
- PCR conditions
- 1uL of template (miniprepped BleoC in pSBA13, and NEB lambda DNA)
- annealing temp = 55*C
- extension time = 3min
- 30 cycles
(1kb standard, BleoC PCR product, Stf PCR product)
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