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Team:ESBS-Strasbourg/21 July 2008
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(New page: < back == DryLab == === Modeling === == WetLab == * Miniprep of TetR (MM) == General == == Quote of the day ==) |
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== WetLab == | == WetLab == | ||
* Miniprep of TetR (MM) | * Miniprep of TetR (MM) | ||
| - | + | * Overnight culture of p1010 A2 (LB+Amp) and p1010 K3 (LB+Kan), i.e. 200µl of little overnight culture in 100 ml medium | |
| + | * Overnight culture of Top10 cells from Invitrogen (cultivated on a plate) to make them competent tomorrow | ||
| + | * PCR of VP16 (3 different templates) Tup1,LacI,CI,TetR @ 48,50,52,53 °C -> none worked | ||
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Latest revision as of 10:50, 24 July 2008
Contents |
DryLab
Modeling
WetLab
- Miniprep of TetR (MM)
- Overnight culture of p1010 A2 (LB+Amp) and p1010 K3 (LB+Kan), i.e. 200µl of little overnight culture in 100 ml medium
- Overnight culture of Top10 cells from Invitrogen (cultivated on a plate) to make them competent tomorrow
- PCR of VP16 (3 different templates) Tup1,LacI,CI,TetR @ 48,50,52,53 °C -> none worked
