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Team:The University of Alberta/23 July 2008
From 2008.igem.org
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*Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase); 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase). | *Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase); 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase). | ||
*Minipreped TetR O/N's set up on Tuesday night. It is in MininPrep Box 3. | *Minipreped TetR O/N's set up on Tuesday night. It is in MininPrep Box 3. | ||
| - | *Digested B0034 with Spe/Pst | + | *Digested B0034 with Spe/Pst; TetR with XbaI/Pst. Digested the biobrick Lac1-ERE with Spe/Pst. Running them on 1% gel. |
| + | **Gel purified the digestions. | ||
| + | *Ligated TetR with RBS (B0034). | ||
| + | *Digested pTet (R0400) with Xba/Pst. | ||
Latest revision as of 22:49, 23 July 2008
- Gel purification of 1) conc. double digests of BisdA and BisdB with both (NgoMIV+Age1) and (XbaI+PstI); 2)conc. digest of vector containing the TetR binding site and the RBS with NgoMIV (digest was treated with Antartic Phosphatase); 3) conc. digest of J61003 with (SpeI+PstI) (digest was treated with Antartic Phosphatase).
- Minipreped TetR O/N's set up on Tuesday night. It is in MininPrep Box 3.
- Digested B0034 with Spe/Pst; TetR with XbaI/Pst. Digested the biobrick Lac1-ERE with Spe/Pst. Running them on 1% gel.
- Gel purified the digestions.
- Ligated TetR with RBS (B0034).
- Digested pTet (R0400) with Xba/Pst.
