Team:University of Ottawa/23 July 2008

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(New page: __TOC__ ==Today in the lab== '''Dan''' :'''PCR amplification of T123''' ::<li> I wanted to do PCR amplification of 0A and 0B, however PCR blocks were full, I will do this tomorrow ::<li> 6...)
(→Today in the lab)
 
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__TOC__
__TOC__
==Today in the lab==
==Today in the lab==
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:'''Transformation of p2S and p2D'''
:'''Transformation of p2S and p2D'''
::<li>p2S and p2D were transformed in E. coli
::<li>p2S and p2D were transformed in E. coli
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'''Chris'''
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:'''Minipreparation of AtCRE'''
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::<li> Used minprep kit and protocol to isolate AtCRE from inoculated cells.
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::<li> Measured absorbance of resulting DNA samples to determine concentrations
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:'''Confirmation of AtCRE'''
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::<li> Performed a digestion of AtCRE with EcoRI against a water control and a positive control (DQ2325601 digested with EcoRI)
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::<li> Ran product on a 1% gel for 40 minutes at 80V
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::<li> Two of six samples showed desired bands.
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'''Matt'''
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:'''Ligation of PTP2 with pSSA42'''
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::<li>Attempted another ligation of PTP2/pSSA42 this time with 1:1, 3:1, Vector with ligase, H2O, Vector without ligase.

Latest revision as of 18:34, 7 August 2008

Untitled Document

 

 


Contents

Today in the lab

Dan

PCR amplification of T123
  • I wanted to do PCR amplification of 0A and 0B, however PCR blocks were full, I will do this tomorrow
  • 6 tubes of PCR reaction were prepared for T123, hopefuly this will give me the amount of DNA that I need for a successful ligation.
    • Transformation of p2S and p2D
  • p2S and p2D were transformed in E. coli

    • Chris

    Minipreparation of AtCRE
  • Used minprep kit and protocol to isolate AtCRE from inoculated cells.
  • Measured absorbance of resulting DNA samples to determine concentrations
    • Confirmation of AtCRE
  • Performed a digestion of AtCRE with EcoRI against a water control and a positive control (DQ2325601 digested with EcoRI)
  • Ran product on a 1% gel for 40 minutes at 80V
  • Two of six samples showed desired bands.

    • Matt

    Ligation of PTP2 with pSSA42
  • Attempted another ligation of PTP2/pSSA42 this time with 1:1, 3:1, Vector with ligase, H2O, Vector without ligase.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/23_July_2008"