Latest revision as of 03:32, 26 July 2008

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Things we did today

Wetlab work

Gel for Plasmid Prep from Yesterday

Margaret

File:Plasmid prep.pdf

Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.

Biobrick Extraction

Margaret

extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37°C incubation, but we will not see the results of this because of my antibiotics mix-up
on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked
on 7/25 the re-streaked plates were placed in 4°C.

Plasmid Prep pRL1383a-M

Krystle

200 ml prep of the new pRL1383 plasmid with biobrick multiple cloning site

Checked for transformants from yesterday/restreaked

Krystle

GFP + tt = 5 colonies
GFP fusion + tt = 15 colonies
nir + rbs = 1 colony

Discussion

Quote of the Day

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Gel for Plasmid Prep from [[Team:Hawaii/Notebook/2008-07-22|Yesterday]]===
 :<strong> Margaret </strong>
+[[Image:plasmid_prep.pdf|right|thumb|300px|Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.]]
-[[Image:plasmid_prep.jpg|right|thumb|300px|plasmid prep]]
+===Biobrick Extraction===
-:* e.g. worked on &lt;blah experiment link&gt;, PCR, ran gel
+:<strong>Margaret</strong>
-== Drylab Work ==
+:*extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37&deg;C incubation, but we will not see the results of this because of my antibiotics mix-up
+:*on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked
+:*on 7/25 the re-streaked plates were placed in 4&deg;C.
-===Name of Task===
+===Plasmid Prep pRL1383a-M===
-:<strong> name of person/people who performed the task</strong>
+:<strong> Krystle </strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+:*200 ml prep of the new pRL1383 plasmid with biobrick multiple cloning site
-:* e.g. read through papers, worked on proposal, etc.
+===Checked for transformants from yesterday/restreaked===
+:<strong> Krystle </strong>
+:* GFP + tt = 5 colonies
+:* GFP fusion + tt = 15 colonies
+:* nir + rbs = 1 colony
 = Discussion =
 = Quote of the Day =
-<blockquote>''History is the only laboratory we have in which to test the consequences of thought.'' - Étienne Gilson</blockquote>
 {{Team:Hawaii/Footer}}
 __NOTOC__

Team:Hawaii/Notebook/2008-07-23

From 2008.igem.org