Team:BCCS-Bristol/Calendar-Notebook/1 July 2008

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Latest revision as of 09:50, 14 September 2008

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This is a page for any solutions

Media for swimming agar assay plates	0.3% 400ml	0.15% 150ml	0.5% 150ml

Bactotryptone	4g	1.5g	1.5g
NaCl	2g	0.75g	0.75g
BactoAgar	1.2g	0.225g	0.75g

in addition did a streak out for master colonies of MG1655 in LB from table below overnight at 37oC

SOC medium in preparation for Biobrick transformation this consisted of

in 95ml of water, then autoclaved. after this add this:

then freeze at -20^oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.

Swimming Medium

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-<html><link rel="stylesheet" href="http://www.chofski.co.uk/iGEM/bccs-igem.css" type="text/css"></html>
+<html><link rel="stylesheet" href="http://homepage.mac.com/tgorochowski/iGEM/bccs-igem.css" type="text/css"></html>
 __NOTOC__
 <div class="bccsNavBar">
@@ Line 18: / Line 18: @@
 {| {{table}}
-| align="center" style="background:#f0f0f0;"|'''Media for Plates'''
+| align="center" style="background:#f0f0f0;"|'''Media for swimming agar assay plates'''
 | align="center" style="background:#f0f0f0;"|'''0.3% 400ml'''
 | align="center" style="background:#f0f0f0;"|'''0.15% 150ml'''
@@ Line 87: / Line 87: @@
 |}
-then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
+then freeze at -20<sup>o</sup>C. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
@@ Line 103: / Line 103: @@
 | EDTA||0.003722
 |-
-| Tween -20 aka polysorbate 20||0.2 MICRO litres
+| Tween -20 aka polysorbate 20||0.2 µl
 |-
 |
 |}