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Team:Johns Hopkins/Notebook/GROUP 6: Vectors
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== GROUP 6: Vectors == | == GROUP 6: Vectors == | ||
| - | Status report by | + | Status report by Tejas on 8/5/08 |
| - | Vector transformed into bacteria strain DB3.1 | + | Vector transformed into bacteria strain DB3.1: Yes. Pre-transformed by MIT and delivered in STABS |
| - | Permanent culture made in the Boeke lab for future reference | + | Permanent culture made in the Boeke lab for future reference: No |
| - | Selectable marker for this vector | + | Selectable marker for this vector: (Amp, Kan, Cam) |
| - | Medium made and tested | + | Medium made and tested: No (link) |
| - | DNA preps made | + | DNA preps made: Yes |
| - | DNA preps tested by RE digest | + | DNA preps tested by RE digest: Yes, [http://www.jhu.edu/iGEM/Group6:Vectors/2008-7-24.Vectors%20(CAM,AMP,KAN3,KAN4)%20after%20Digest.Tejas.html Vectors (CAM,AMP,KAN3,KAN4) after Digest] |
| - | DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) | + | DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109): No (currently being done) |
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Sample format/data follows:<br> | Sample format/data follows:<br> | ||
Amount transformed cfu/micGm in DB3.1 cfu/micGm in JM109 | Amount transformed cfu/micGm in DB3.1 cfu/micGm in JM109 | ||
0.1 ng 5 * 10e7 <2 * 10e2<br> | 0.1 ng 5 * 10e7 <2 * 10e2<br> | ||
Preparative digests ready for use are located – where? | Preparative digests ready for use are located – where? | ||
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Latest revision as of 21:06, 5 August 2008
GROUP 6: Vectors
Status report by Tejas on 8/5/08 Vector transformed into bacteria strain DB3.1: Yes. Pre-transformed by MIT and delivered in STABS Permanent culture made in the Boeke lab for future reference: No Selectable marker for this vector: (Amp, Kan, Cam) Medium made and tested: No (link) DNA preps made: Yes DNA preps tested by RE digest: Yes, [http://www.jhu.edu/iGEM/Group6:Vectors/2008-7-24.Vectors%20(CAM,AMP,KAN3,KAN4)%20after%20Digest.Tejas.html Vectors (CAM,AMP,KAN3,KAN4) after Digest] DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109): No (currently being done) Sample format/data follows:
Amount transformed cfu/micGm in DB3.1 cfu/micGm in JM109 0.1 ng 5 * 10e7 <2 * 10e2
Preparative digests ready for use are located – where?
