Latest revision as of 03:32, 26 July 2008

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Things we did today

Margaret

File:Plasmid prep.pdf

Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.

Margaret

extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37°C incubation, but we will not see the results of this because of my antibiotics mix-up
on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked
on 7/25 the re-streaked plates were placed in 4°C.

Krystle

Krystle

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Gel for Plasmid Prep from [[Team:Hawaii/Notebook/2008-07-22|Yesterday]]===
 :<strong> Margaret </strong>
+[[Image:plasmid_prep.pdf|right|thumb|300px|Plasmid Prep of pSB1A3, pSB1A2, pSB3K3, and a verification of pSMC121.]]
+===Biobrick Extraction===
+:<strong>Margaret</strong>
+:*extracted: I14032, pSB1A7, I51020, J23012 and transformed into DB3.1 cells. Plated on amp100. **the problem is that I14032 is Kan resistant. I introduced 1mM IPTG to the 2 hour 37&deg;C incubation, but we will not see the results of this because of my antibiotics mix-up
+:*on 7/24 there were 2 colonies on the pSB1A7 plate and 4 colonies on the I51020 plate, these were restreaked
+:*on 7/25 the re-streaked plates were placed in 4&deg;C.
 ===Plasmid Prep pRL1383a-M===