Team:Hawaii/Parts

From 2008.igem.org

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===Guidelines for naming parts===
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==Guidelines for naming parts==
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===Parts planned===
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{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
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|-
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! width="100"|Part number
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! width="350"|Part type
 +
|- style="background:#f0f0f0; color:black"
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| '''K125000-K125099'''
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| align="center"|plasmid
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|- style="background:#f0f0f0; color:black"
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| '''K125100-K125199'''
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| align="center"|promoter
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|- style="background:#f0f0f0; color:black"
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| '''K125200-K125299'''
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| align="center"|ribosome binding site
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|- style="background:#f0f0f0; color:black"
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| '''K125300-K125499'''
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| align="center"|signal sequences, conjugation sequences, random stuff
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|- style="background:#f0f0f0; color:black"
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| '''K125500-K125699'''
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| align="center"|protein coding
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|- style="background:#f0f0f0; color:black"
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| '''K125700-K125799'''
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| align="center"|transcription terminator
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|- style="background:#f0f0f0; color:black"
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| '''K125800-K125999'''
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| align="center"|devices
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|}
 +
 
 +
==Parts planned==
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===Parts in process (building)===
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==Parts in process (building)==
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
|-
|-
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! width="75"|Type
! width="75"|Type
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
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| '''K125000'''
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
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| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_B0034 (rbs)
+
| Broad-Host-Range plasmid derived from RSF1010
| plasmid
| plasmid
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
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| '''K125100'''
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125330 '''K125330''']
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| P1 lytic replication
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|
 +
|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125340 '''K125340''']
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| oriV
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|
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125510 '''K125510''']
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| Plasmid replication proteins
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| protein coding
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125520 '''K125520''']
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| aadA gene
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| protein coding
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125800 '''K125800''']
 +
| rep proteins with promoter and rbs
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| protein coding
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125830 '''K125830''']
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| ''Synechocystis''-specific, nitrate-inducible GFP secretion device under the control of a ''nirA'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
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| device
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|}
 +
 
 +
==Parts submitted==
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{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
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|-
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! width="75"|Part number
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! width="250"|Description
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! width="75"|Type
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000''']
 +
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (''lac'' promoter+''lacZ'')
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| plasmid
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
| nitrate inducible ''nir'' promoter
| nitrate inducible ''nir'' promoter
| regulator
| regulator
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
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| '''K125300'''
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110''']
 +
| nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030]
 +
| regulator
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125300 '''K125300''']
| cyanobacterial pilA derived amino termninus protein secretion signal sequence
| cyanobacterial pilA derived amino termninus protein secretion signal sequence
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
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| '''K125310'''
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 '''K125310''']
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
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| '''K125500'''
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
| GFP fusion protein
| GFP fusion protein
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| protein coding
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810''']
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| N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| protein coding
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|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820''']
 +
| ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
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| device
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
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| oriTr/LacZ
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| conjugation screening
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125360 '''K125360''']
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| oriT/LacZ
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| conjugation screening
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
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| oriT derived from RP4
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| origin of transfer
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125020 '''K125020''']
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| rep/oriv/aada/orit
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| intermediate for RSF1010 derived plasmid
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|- style="background:#f0f0f0; color:black"
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| [http://partsregistry.org/wiki/index.php/Part:BBa_K125530 '''K125530''']
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| aadA gene with promoter and rbs
| protein coding
| protein coding
|}
|}
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===Parts submitted===
 

Latest revision as of 03:53, 30 October 2008

Contents

Guidelines for naming parts

Part number Part type
K125000-K125099 plasmid
K125100-K125199 promoter
K125200-K125299 ribosome binding site
K125300-K125499 signal sequences, conjugation sequences, random stuff
K125500-K125699 protein coding
K125700-K125799 transcription terminator
K125800-K125999 devices

Parts planned

Parts in process (building)

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125010 K125010] Broad-Host-Range plasmid derived from RSF1010 plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125330 K125330] P1 lytic replication
[http://partsregistry.org/wiki/index.php/Part:BBa_K125340 K125340] oriV
[http://partsregistry.org/wiki/index.php/Part:BBa_K125510 K125510] Plasmid replication proteins protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125520 K125520] aadA gene protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125800 K125800] rep proteins with promoter and rbs protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125830 K125830] Synechocystis-specific, nitrate-inducible GFP secretion device under the control of a nirA promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device

Parts submitted

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125000 K125000] pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (lac promoter+lacZ) plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100] nitrate inducible nir promoter regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125110] nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125300 K125300] cyanobacterial pilA derived amino termninus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310] cyanobacterial slr2016 derived amino terminus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500] GFP fusion protein protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125810 K125810] N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125820 K125820] Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device
[http://partsregistry.org/wiki/index.php/Part:BBa_K125350 K125350] oriTr/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125360 K125360] oriT/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125320 K125320] oriT derived from RP4 origin of transfer
[http://partsregistry.org/wiki/index.php/Part:BBa_K125020 K125020] rep/oriv/aada/orit intermediate for RSF1010 derived plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125530 K125530] aadA gene with promoter and rbs protein coding
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