Team:Hawaii/Construction of pRL1383a

From 2008.igem.org

(Difference between revisions)
m (New page: {{Team:Hawaii/Header}} == Construction of pRL1383a == * The BioBrick parts of pRL1383a are to be ligated in a series of experiments. == Methods == <strong> Round 1 of Restriction Diges...)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Hawaii/Header}}
{{Team:Hawaii/Header}}
-
== Construction of pRL1383a ==
+
==Ligation of Parts==
* The BioBrick parts of pRL1383a are to be ligated in a series of experiments.  
* The BioBrick parts of pRL1383a are to be ligated in a series of experiments.  
 +
 +
== Methods ==
== Methods ==
-
<strong> Round 1 of Restriction Digest and Ligation </strong>
+
#<strong> Round 1 of Restriction Digest and Ligation </strong>
{| border="1"
{| border="1"
Line 65: Line 67:
|}
|}
-
<strong> Round 2 of Restriction Digest and Ligation </strong>
+
#In gel purification of ligation products
 +
 
 +
#<strong> Round 2 of Restriction Digest and Ligation </strong>
{| border="1"
{| border="1"
Line 76: Line 80:
|-
|-
|TT-mob  
|TT-mob  
-
|
+
|EcoRI
-
|
+
|SpeI
-
|
+
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
-
|
+
|TT-mob-TT
|-
|-
-
|
+
|P<sub>lac</sub>-aadA
 +
|EcoRI
 +
|SpeI
 +
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
 +
|P<sub>lac</sub>-aadA -TT
 +
|-
 +
|[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
 +
|EcoRI
 +
|XbaI
 +
|see mob and aadA above
 +
|see mob and aadA above
 +
|}
 +
 
 +
#Transformation
 +
 
 +
#Verification with Colony PCR
 +
 
== Results ==
== Results ==

Latest revision as of 02:36, 6 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Contents

Ligation of Parts

  • The BioBrick parts of pRL1383a are to be ligated in a series of experiments.


Methods

  1. Round 1 of Restriction Digest and Ligation
Restriction Digest & Ligation: Round 1
Name Digest 1 Digest 2 Ligation to Product
mob XbaI & PstI n/a [http://partsregistry.org/Part:BBa_B0024 BBa_B0024] TT-mob
rep XbaI & PstI n/a [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Plac-rep
oriV XbaI & PstI n/a [http://partsregistry.org/Part:pSB1A3 pSB1A3] pSB1A3::oriV
aadA region XbaI & PstI n/a [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Plac-aadA
oriT XbaI & PstI n/a [http://partsregistry.org/Part:pSB1A3 pSB1A3] pSB1A3::oriT
pSB1A3 XbaI & PstI n/a see oriT and oriV see oriT and oriV
[http://partsregistry.org/Part:BBa_B0024 BBa_B0024] SpeI PstI see mob region above see mob region above
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] SpeI PstI see rep and aadA region above see rep and aadA region above
  1. In gel purification of ligation products
  1. Round 2 of Restriction Digest and Ligation
Restriction Digest & Ligation: Round 2
Name Digest 1 Digest 2 Ligation to Product
TT-mob EcoRI SpeI [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] TT-mob-TT
Plac-aadA EcoRI SpeI [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Plac-aadA -TT
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015] EcoRI XbaI see mob and aadA above see mob and aadA above
  1. Transformation
  1. Verification with Colony PCR

Results


Discussion

  • What was learned and how to do future experiments differently.


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

Retrieved from "http://2008.igem.org/Team:Hawaii/Construction_of_pRL1383a"