Team:Paris/Parts

From 2008.igem.org

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{{Paris/Menu}}
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[[Team:Paris/Parts2|Not in the registry]]
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=Parts Submitted to the Registery=
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==Basic Parts==
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===[[Image:Part_icon_regulatory.png]] Regulatory===
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=== Parts Submitted n°1 ===
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====Promoter of fliL gene from ''E.coli'' flagella====
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*[http://partsregistry.org/Part:BBa_K136000 BBa_K136000]
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*Sequencing : NO
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*References :
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[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.
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[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720
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[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.
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[[Image:Part_icon_regulatory.png]][[Image:icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_rbs.png]]
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====Promoter of flgA gene from ''E. coli'' flagella====
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*[http://partsregistry.org/Part:BBa_K136001 BBa_K136001]
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*Sequencing : OK
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*References :
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[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.
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pFliL--OmpR
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[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720
 +
[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.
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[[Image:Part_icon_regulatory.png]][[Image:icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
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====Promoter of flgB gene from ''E. coli'' flagella====
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*[http://partsregistry.org/Part:BBa_K136002 BBa_K136002]
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*Sequencing : NO
 +
*References :
 +
[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.
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pFliL--EnvZ
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[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720
 +
[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.
 +
====Promoter of flhB gene from ''E. coli'' flagella====
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*[http://partsregistry.org/Part:BBa_K136003 BBa_K136003]
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*Sequencing : NO
 +
*References :
 +
[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.
 +
[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720
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=== Parts Submitted n°2 ===
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[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.
 +
====Promoter of flhDC gene from ''E. coli'' flagella====
 +
*[http://partsregistry.org/Part:BBa_K136004 BBa_K136004]
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*Sequencing : NO
 +
*References :
 +
[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.
 +
[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720
 +
[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.
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[[Image:Part_icon_regulatory.png]][[Image:icon_coding.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_rbs.png]] :::[[Image:Part_icon_rbs.png]] ?
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=Parts not Submitted to the Registery=
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===[[Image:Icon_coding.png]] Protein coding===
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====FlhDC gene from ''E.coli'' flagella with natural RBS====
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*[http://partsregistry.org/Part:BBa_K136005 BBa_K136005]
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pTet--FlhDC<br>pLac?
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====Coding sequence for FliA with RBS====
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====Coding sequence for FlhDC without RBS====
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====Coding sequence for FliA without RBS====
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====Coding sequence for EnvZ*====
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====Coding sequence for OmpR*====
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==Intermediate Constructions==
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==Finished Constructions==
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[[Image:Part_icon_regulatory.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]],[[Image:Part_icon_reporter.png]] ?,  [[Image:Part_icon_rbs.png]] ?
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pFliA--FliA<br>pLac?<br>pTet?
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[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
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pFliL<br>pFlgA<br>pFlgB<br>pFlhB
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[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
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:::TetR
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pFliL<br>pFlgA<br>pFlgB<br>pFlhB
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[[Image:Part_icon_regulatory.png]][[Image:icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
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: OmpR<br>[[Image:icon_coding.png]]
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:or EnvZ'<br>
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pFliL<br>pFlgA<br>pFlgB<br>pFlhD
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=== Parts Submitted n°3 ===
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[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]]
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[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_terminator.png]]
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[[Image:Part_icon_regulatory.png]][[Image:icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
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::[[Image:icon_coding.png]]
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::[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]]
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[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]]
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Latest revision as of 06:48, 30 October 2008

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Not in the registry

Contents

Parts Submitted to the Registery

Basic Parts

Part icon regulatory.png Regulatory

Promoter of fliL gene from E.coli flagella

  • [http://partsregistry.org/Part:BBa_K136000 BBa_K136000]
  • Sequencing : NO
  • References :

[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.

[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720

[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.

Promoter of flgA gene from E. coli flagella

  • [http://partsregistry.org/Part:BBa_K136001 BBa_K136001]
  • Sequencing : OK
  • References :

[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.

[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720

[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.

Promoter of flgB gene from E. coli flagella

  • [http://partsregistry.org/Part:BBa_K136002 BBa_K136002]
  • Sequencing : NO
  • References :

[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.

[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720

[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.

Promoter of flhB gene from E. coli flagella

  • [http://partsregistry.org/Part:BBa_K136003 BBa_K136003]
  • Sequencing : NO
  • References :

[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.

[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720

[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.

Promoter of flhDC gene from E. coli flagella

  • [http://partsregistry.org/Part:BBa_K136004 BBa_K136004]
  • Sequencing : NO
  • References :

[1] Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science. 2001 Jun 15;292(5524):2080-3.

[2] Shiraz Kalir, Uri Alon. Using quantitative blueprint to reprogram the dynamics of the flagella network. Cell, June 11, 2004, Vol.117, 713-720

[3] Kalir S, Mangan S, Alon U. A coherent feed-forward loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.

Parts not Submitted to the Registery

Icon coding.png Protein coding

FlhDC gene from E.coli flagella with natural RBS

  • [http://partsregistry.org/Part:BBa_K136005 BBa_K136005]

Coding sequence for FliA with RBS

Coding sequence for FlhDC without RBS

Coding sequence for FliA without RBS

Coding sequence for EnvZ*

Coding sequence for OmpR*

Intermediate Constructions

Finished Constructions