| Table of Oligos
|
| Number
| Name
| Sequence
| Length
| Tm(°C)
| Comments
|
| O100
| FlgA-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT
| 58
|
|
|
| O101
| FlgA-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
| 58
|
|
|
| O102
| FlgB-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG
| 58
|
|
|
| O103
| FlgB-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
| 58
|
|
|
| O104
| FlgK-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG
| 58
|
|
|
| O105
| FlgK-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT
| 58
|
|
|
| O106
| FlgM-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA
| 58
|
|
|
| O107
| FlgM-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT
| 58
|
|
|
| O108
| FlhB-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
| 58
|
|
|
| O109
| FlhB-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
| 58
|
|
|
| O110
| FlhDC-Uri-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG
| 58
|
| Don't amplify the both OmpR binding site
|
| O111
| FlhDC-Total-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA
| 58
|
| Amplify the both OmpR binding site
|
| O112
| FlhDC(rbs)-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
| 54
|
| Amplify the natural rbs of FlhD
|
| O113
| FlhDC(rbs)-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG
| 54
|
| Amplify the natural rbs of FlhD
|
| O114
| xx FliA-PCR1-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC
| 58
|
|
|
| O115
| xx FliA-PCR1-R
| CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG
| 59
|
|
|
| O116
| xx FliA-PCR2-F
| GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG
| 42
|
|
|
| O117
| xx FliA-PCR2-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCA
GCGGTATAGAGTGAACTCACGATAAA
| 69
|
|
|
| O118
| FliC-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT
| 58
|
|
|
| O119
| FliC-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT
| 58
|
|
|
| O120
| FliE-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG
| 58
|
|
|
| O121
| FliE-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA
| 58
|
|
|
| O122
| FliF-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
| 58
|
|
|
| O123
| FliF-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTCGCATTCATCGCGCACCTCGTGGCTGA
| 58
|
|
|
| O124
| Oligo-pfliL-Forward-TRO
| TCGAATTCGCGGCCGCTTCTAGAGCAAGGGCGTGTAACAGGCAAC
| 45
| 60°C
|
|
| O125
| Oligo-pfliL-Reverse-TRO
| TCCTGCAGCGGCCGCTACTAGTAGTCATGTGTTGCGGTCTTCCTGTG
| 47
| 62°C
|
|
| O126
| Gene-EnvZ-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC
| 53
|
|
|
| O127
| Gene-EnvZ-R-TRO
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACCCTTCTTTTGTCGTGCCCTGCGCC
| 60
| 68°C
|
|
| O128
| Gene-FlgM-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC
| 53
|
|
|
| O129
| Gene-FlgM-R-TRO
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
| 59
| 59°C
|
|
| O130
| Gene-FlhC-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG
| 53
|
|
|
| O131
| Gene-FlhC-R-TRO
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC
| 60
| 60°C
|
|
| O132
| Gene-FlhD-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC
| 53
|
| Don't amplify the natural rbs of FlhD
|
| O133
| Gene-FlhD-R-TRO
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
| 60
| 68°C
|
|
| O134
| Gene-FlhDCrbs-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT
| 53
|
| Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
|
| O135
| xx Gene-FliA-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
| 53
|
| Don't Amplify the natural rbs of FliA
|
| O136
| xx Gene-FliA-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC
| 59
|
|
|
|
| O137
| xx Gene-FliArbs-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
| 53
|
| Amplify the natural rbs of FliA
|
| O138
| Gene-OmpR-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG
| 53
|
|
|
| O139
| Gene-OmpR-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATGCTTTAGAGCCGTCCGGTACAAAG
| 60
| 62°C
|
|
| O140
| PlasTest_GFP_Rv
| CCCTGCAGTTAATTAATATAAACGCAGAAAGGCCAC
| 36
| 54
| Amplify E0240 with a PacI and a PstI restriction site at the end
|
| O141
| PTst_GFPrbs+_F
| GCCTGCAGTCACACAGGAAAGTACTAGATGC
| 31
| 56
| Amplify E0240 with the promoter and a PstI restriction site at the beginning
|
| O142
| PTst_GFPrbs-_F
| GCCTGCAGATGCGTAAAGGAGAAGAACTTTTC
| 32
| 55
| Amplify E0240 without the promoter and a PstI restriction site at the beginning
|
| O143
| G-PCRmut-1F
| GGCTGTTTATCGTGAACTCACTCTATACCG
| 30
| 62
| Mutate the EcoRI site of FliA ( without biobrick prefix)
|
| O144
| P-FliArbs-R
| CTTCCTGCAGCGGCCGCTACTAGTATTAAACAGCCCTGCGTTATATG
| 47
| 55 - 72
| Amplify the rbs of FliA
|
| O145
| P-xb-FliA-F
| GCTTCTAGAGCCTGATTAACTGAGACTGAC
| 30
| 51 - 62
| No EcoRI site on prefix for amplifying promoter
|
| O146
| P-FliA-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATATGAGTTATCGGCATGAT
| 51
| 51 - 71
|
|
| O147
| G-xb-FliA-F
| CTTCTAGAACGCAGGGCTGTTTATCGTG
| 28
| 57 - 63
| Amplify the gene with its natural rbs
|
| O148
| G-mecoFliA-F
| TCGAATTCGCGGCCGCTTCTAGACAGGGCTGTTTATCGTGAACTCACTCTATA
| 53
| 61 - 74
| Oligo for second run PCR
|
| O149
| G-spe-FliA-R
| GCTACTAGTATTATTATAACTTACCCAGTTTAGTGCG
| 37
| 53 - 60
| Oligo FliA for non-mutated sequence
|
| O150
| G-FliA-R
| TCCTGCAGCGGCCGCTACTAGTATTATTATAACTTACCCAGTTTAGTGCG
| 50
| 53 - 71
| Oligo for complete mutated sequence - second run of PCR
|
| O151
| PG-xb-FliA-R
| GCTTCTAGAATTATTATAACTTACCCAGTTTAGTGCG
| 37
| 53 - 61
| Oligo reverse with xbaI site for non mutated sequence
|
| O152
| G-PCRmut-2R
| GGGCAGTCGAACTTGCAGGCGCAATGCTTC
| 30
| 71
| Oligo for first run of PCR, mutate PstI.
|
| O153
| G-PCRmut-3F
| GAAGCATTGCGCCTGCAAGTTCGACTGCCC
| 30
| 71
| Oligo for fisrt run of PCR, mutate PstI.
|
| O154
| pFlhDC-tto-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCTGTCGTTCTCAAAATCGGTG
| 55
| 62 - 75
|
|
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