Team:Valencia/Notebook/October

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{{Valencia/Menu}}
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[[Team:Valencia|<font color="#047DB5">Home</font>]]
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Team|<font color="#047DB5">The Team</font>]]
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Project|<font color="#047DB5">The Project</font>]]
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Parts|<font color="#047DB5">Parts Submitted to the Registry</font>]]
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Modeling|<font color="#047DB5">Modeling</font>]]
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&nbsp;&nbsp;|&nbsp;&nbsp;[[Team:Valencia/Notebook|<font color="#047DB5">Notebook</font>]]
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{| 
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/April|<font color="#047DB5">April</font>]]</Center>
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|- bgcolor="#FFE099"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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{| 
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|- bgcolor="#FFE099"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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{| 
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/June|<font color="#047DB5">June</font>]]</Center>
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|- bgcolor="#FFE099"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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| <Font Color="#c0c0c0">26</Font> || <Font Color="#c0c0c0">27</Font> || &nbsp;<Font Color="#c0c0c0">28</Font> || &nbsp;<Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || <Font Color="#c0c0c0">31</Font> || &nbsp;1
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<!-- {{{Año}}}/07 (Julio) -->
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{| {{tablabonita}}
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{|
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/July|July]]</Center>
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/July|<font color="#047DB5">July</font>]]</Center>
|- bgcolor="#FFE099"
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/August|August]]</Center>
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/August|<font color="#047DB5">August</font>]]</Center>
|- bgcolor="#FFE099"
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/September|September]]</Center>
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/September|<font color="#047DB5">September</font>]]</Center>
|- bgcolor="#FFE099"
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| colspan="7" style="background:#FFB428;" | <Center>[[Team:Valencia/Notebook/October|October]]</Center>
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| colspan="7" style="background:#FFB428;" | <Center>[[Team:Valencia/Notebook/October|<font color="#047DB5">October</font>]]</Center>
|- bgcolor="#FFE099"
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| <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;&nbsp;[[Team:Valencia/Notebook/October#October 1st | 1]]|| &nbsp;[[Team:Valencia/Notebook/October#October 2nd | 2]] || &nbsp;3 || &nbsp;4 || &nbsp;5
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| &nbsp;6 || &nbsp;[[Team:Valencia/Notebook/October#October 7th |7]]  || &nbsp;&nbsp;8|| 9 || 10 || 11|| 12
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| [[Team:Valencia/Notebook/October#October 13th |13]] || [[Team:Valencia/Notebook/October#October 14th |14]]  || &nbsp;15 || 16 || [[Team:Valencia/Notebook/October#October 17th |17]] || 18 || 19
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| [[Team:Valencia/Notebook/October#October 20th |20]] || 21 || &nbsp;22 || [[Team:Valencia/Notebook/October#October 23rd |23]]  || [[Team:Valencia/Notebook/October#October 24th |24]]  || 25 || 26  
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/November|November]]</Center>
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| colspan="7" style="background:#FFD062;" | <Center>[[Team:Valencia/Notebook/November|<font color="#047DB5">November</font>]]</Center>
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<!-- ESCRIBIR AQUI -->
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=== October 1st ===
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- We repeated the previous experiment:<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 2 <br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC.<br>
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=== October 2nd ===
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- Team meeting: We talked about the biobricks and some experiments left.
 +
 +
=== October 7th ===
 +
- We repeated the previous experiment:<br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 2 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC.<br>
 +
 +
=== October 13th ===
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- Total DNA was extracted from our yeast strains <br>
 +
- UCP-1 was amplified by [[Team:Valencia/Parts#Preparing_inserts|PCR]] using oligonucleotides matching the sequence and bearing the appropriate Biobrick prefix and suffix.
 +
 +
=== October 14th ===
 +
- We performed an [[Team:Valencia/Parts#Preparing_inserts|agarose gel electrophoresis]] in order to check our PCR, the results was: Intense amplicons, of the expected size, (about 1 kb) were produced for UCP-1, 175-deleted and 76 deleted.
 +
 +
=== October 17th ===
 +
- Team meeting: We discussed several things that need to be finished, such as sending DNA, preparing presentation, poster, wiki...
 +
 +
=== October 20th ===
 +
- Preparing vectors, competent cells were transformed with: pSB1AK3 and pUC18 plasmids.
 +
 +
=== October 23rd ===
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- Plasmids were extracted (High pure miniprep plasmid isolation kit ROCHE).
 +
 +
- Plasmids were digested with EcoRI and PstI
 +
 +
=== October 24th ===
 +
- [[Team:Valencia/Parts#Ligating_Biobricks_into_plasmids|Ligating Biobricks]] into plasmids:
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Inserts were run into agarose gels and DNA bands excised with a clean scalpel. DNA was extracted from agarose blocks (ultra clean gel spin, DNA purification Kit, MO BIO laboratories). T4 Ligase was used to ligate inserts and vectors.
 +
 +
- Competent cells were transformed and resulting colonies (Amp LB) screened with Fw and Rv primers to confirm the presence of inserts.
 +
 +
=== October 27th ===
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- pSB1AK3 containing UCP-1, 175-deleted and 76-deleted were sent to the Registry.
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<html>
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<h2>
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Back to<a href="https://2008.igem.org/Team:Valencia/Notebook/September"><font color="#047DB5"> September </font></a>&nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;October &nbsp;&nbsp;&nbsp;| &nbsp;&nbsp;&nbsp;Go to <a href="https://2008.igem.org/Team:Valencia/Notebook/November"><font color="#047DB5"> November </font></a></h2>
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Latest revision as of 22:14, 29 October 2008



April
Mo Tu We Th Fr Sa Su
31  1   2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30  1  2  3  4
May
Mo Tu We Th Fr Sa Su
28 29  30  1  2  3  4
 5  6   7  8  9 10 11
12 13  14 15 16 17 18
19 20  21 22 23 24 25
26 27  28 29 30 31 1
June
Mo Tu We Th Fr Sa Su
26 27  28  29 30 31  1
 2  3   4  5  6  7  8
 9 10  11 12 13 14 15
16 17  18 19 20 21 22
23 24  25 26 27 28 29
30  1   2  3  4  5  6
July
Mo Tu We Th Fr Sa Su
30  1  2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30 31   1  2  3
August
Mo Tu We Th Fr Sa Su
28 29  30 31  1  2  3
 4  5   6  7  8  9 10
11 12  13 14 15 16 17
18 19  20 21 22 23 24
25 26  27 28 29 30 31
September
Mo Tu We Th Fr Sa Su
 1  2   3  4  5  6  7
 8  9  10 11 12 13 14
15 16  17 18 19 20 21
22 23  24 25 26 27 28
29 30  1 2 3 4 5
October
Mo Tu We Th Fr Sa Su
29 30    1   2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2
November
Mo Tu We Th Fr Sa Su
27 28  29 30 31  1  2
 3  4   5  6  7  8  9
10 11  12 13 14 15 16
17 18  19 20 21 22 23
24 25  26 27 28 29 30


October 1st

- We repeated the previous experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 2
      270 rpm.
       Initial temperature 28 ºC.

October 2nd

- Team meeting: We talked about the biobricks and some experiments left.

October 7th

- We repeated the previous experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 2
      270 rpm.
       Initial temperature 28 ºC.

October 13th

- Total DNA was extracted from our yeast strains
- UCP-1 was amplified by PCR using oligonucleotides matching the sequence and bearing the appropriate Biobrick prefix and suffix.

October 14th

- We performed an agarose gel electrophoresis in order to check our PCR, the results was: Intense amplicons, of the expected size, (about 1 kb) were produced for UCP-1, 175-deleted and 76 deleted.

October 17th

- Team meeting: We discussed several things that need to be finished, such as sending DNA, preparing presentation, poster, wiki...

October 20th

- Preparing vectors, competent cells were transformed with: pSB1AK3 and pUC18 plasmids.

October 23rd

- Plasmids were extracted (High pure miniprep plasmid isolation kit ROCHE).

- Plasmids were digested with EcoRI and PstI

October 24th

- Ligating Biobricks into plasmids: Inserts were run into agarose gels and DNA bands excised with a clean scalpel. DNA was extracted from agarose blocks (ultra clean gel spin, DNA purification Kit, MO BIO laboratories). T4 Ligase was used to ligate inserts and vectors.

- Competent cells were transformed and resulting colonies (Amp LB) screened with Fw and Rv primers to confirm the presence of inserts.

October 27th

- pSB1AK3 containing UCP-1, 175-deleted and 76-deleted were sent to the Registry.



Back to September    |    October    |    Go to November


Retrieved from "http://2008.igem.org/Team:Valencia/Notebook/October"