Wisconsin: Lignin Project/3 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: == Team Sorbitol == Created SOC media for transformations. Transformed each of the ligations (as well as controls) into our chemically competent stocks of DH5a. To do this we followed th...) |
|||
(4 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | = | + | <div style="background: #000; padding: 10px;"> |
- | + | ||
- | + | <div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div> | |
- | To do this we followed the protocol of the Weibel Lab. | + | {|align="justify" style="color:#aada84;background-color:#000;" width="800 px" |
- | + | |'''Team Sorbitol:'''<br> | |
- | We plated the transformations on LB/Amp (100ug/mL) overnight at 30C | + | Created SOC media for transformations.<br> |
+ | Transformed each of the ligations (as well as controls) into our chemically competent stocks of DH5a.<br> | ||
+ | To do this we followed the protocol of the Weibel Lab.<br> | ||
+ | We plated the transformations on LB/Amp (100ug/mL) overnight at 30C<br> | ||
+ | further growth curves completed with C-media and TpiA knockout strain JW3890.<br> | ||
+ | '''Team Fungus:'''<br> | ||
+ | Purified pET28a from overnight culture.<br> | ||
+ | Ran out yesterday's 2nd PCR product on 1% agarose gel. Correct band size.<br> | ||
+ | Purified PCR product from yesterday's 2nd PCR.<br> | ||
+ | Digested both purified PCR product and pET28a with EcoRI and XhoI.<br> | ||
+ | Ran digestion products out on gel. pET28a product too big?<br> | ||
+ | Grew another overnight of pET28a.<br> | ||
+ | |} |
Latest revision as of 02:40, 29 October 2008
Team Sorbitol: Created SOC media for transformations. |