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Purdue/17 June 2008
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| + | *First trial of nebulized Sol-Gel | ||
| + | **Coated bottom of petri dish w/ media (alpha-MEM) | ||
| + | **Sprayed for 45s | ||
| + | **Added 4 mL media and 1 mL LB afterwards | ||
| + | *Wait to see if the cells grow | ||
| + | *Got visual confirmation via microscope | ||
| + | **GFP E. coli are alive | ||
| + | **Sol-Gel is flaking off | ||
| + | *Next time attempt with bottom layer of gelatin so Sol-Gel doesn't flake/leak/etc. | ||
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| + | Side note: We were mistaken about the sam8 gene--it is the LacZ gene that encodes Beta-gal which cleaves X-gal to make a blue color. The sam8 is irrelevant. LacZ can be found as part I732005, and is available in the 2008 shipment of DNA. | ||
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| + | '''Edited by Janie Stine''' | ||
Latest revision as of 18:48, 17 June 2008
Click Here to return to the notebook.
- First trial of nebulized Sol-Gel
- Coated bottom of petri dish w/ media (alpha-MEM)
- Sprayed for 45s
- Added 4 mL media and 1 mL LB afterwards
- Wait to see if the cells grow
- Got visual confirmation via microscope
- GFP E. coli are alive
- Sol-Gel is flaking off
- Next time attempt with bottom layer of gelatin so Sol-Gel doesn't flake/leak/etc.
Side note: We were mistaken about the sam8 gene--it is the LacZ gene that encodes Beta-gal which cleaves X-gal to make a blue color. The sam8 is irrelevant. LacZ can be found as part I732005, and is available in the 2008 shipment of DNA.
Edited by Janie Stine
