Wisconsin:Lignin Project/4 August 2008

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|'''Team Sorbitol:'''<br>
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Began PCR reactions to clone srlD with restriction sites to insert it into the pET28a vector as well as to make it brickable.  The PCR products were verified via electrophoresis and showed the correct sizes.
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'''Team Fungus:'''<br>
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Took 1 ml from all 11 overnight cultures and centrifuged them for 10 min @ 3400 rpm.<br>
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Purified pEt28a using QIAquick Mini Prep kit.<br>
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Ran digestion for all 10 colonies, positive control colony, no DNA, and pET28a (double, single, undigested) using EcoRI and XhoI.<br>
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Ran the gel and got correct digestion bands for colonies 2, 5, 6, and 7.<br>
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Designed and ran sequencing reaction on those four colonies overnight.<br>
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Latest revision as of 16:38, 28 October 2008

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Team Sorbitol:

Began PCR reactions to clone srlD with restriction sites to insert it into the pET28a vector as well as to make it brickable. The PCR products were verified via electrophoresis and showed the correct sizes.

Team Fungus:
Took 1 ml from all 11 overnight cultures and centrifuged them for 10 min @ 3400 rpm.
Purified pEt28a using QIAquick Mini Prep kit.
Ran digestion for all 10 colonies, positive control colony, no DNA, and pET28a (double, single, undigested) using EcoRI and XhoI.
Ran the gel and got correct digestion bands for colonies 2, 5, 6, and 7.
Designed and ran sequencing reaction on those four colonies overnight.

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