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Team:TU Munchen/Projects
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=Product Transport in E. Coli (E. Coli Filter)= | =Product Transport in E. Coli (E. Coli Filter)= | ||
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* '''Goal:''' Make E. coli absorb some product. | * '''Goal:''' Make E. coli absorb some product. | ||
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** A starting point for other metals is [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1W-4JTJ9T5-1&_user=616147&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000032324&_version=1&_urlVersion=0&_userid=616147&md5=a755ffdf33ea1c8d5f687b7385e3f250 Structural Analysis and Classification of Native Proteins from E. Coli Commonly Co-Purified by Immobilised Metal Affinity Chromatography]. | ** A starting point for other metals is [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1W-4JTJ9T5-1&_user=616147&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000032324&_version=1&_urlVersion=0&_userid=616147&md5=a755ffdf33ea1c8d5f687b7385e3f250 Structural Analysis and Classification of Native Proteins from E. Coli Commonly Co-Purified by Immobilised Metal Affinity Chromatography]. | ||
| + | **Bacteria used for extraction of gold "freeing": [http://www.goldfields.co.za/company_technology.asp?navDisplay=Company Called BIOX Process] | ||
=Chemotaxis Control (E. Coli Filter and E. Coli Rugby)= | =Chemotaxis Control (E. Coli Filter and E. Coli Rugby)= | ||
Latest revision as of 20:15, 2 June 2008
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Contents |
Product Transport in E. Coli (E. Coli Filter)
- Goal: Make E. coli absorb some product.
- Questions:
- What products do we want E. coli to absorb?
- How should E. coli absorb the products?
- Notes:
- Someone should research [http://en.wikipedia.org/wiki/Bioremediation bioremediation] to determine what products cause environmental problems.
- We should understand transport across the cell envelope of [http://en.wikipedia.org/wiki/Gram-negative Gram-negative bacteria].
- E. coli do transport iron across their cell envelope. A great review is [http://www.sciencemag.org/cgi/reprint/295/5560/1658.pdf Close Before Opening].
- A starting point for other metals is [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1W-4JTJ9T5-1&_user=616147&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000032324&_version=1&_urlVersion=0&_userid=616147&md5=a755ffdf33ea1c8d5f687b7385e3f250 Structural Analysis and Classification of Native Proteins from E. Coli Commonly Co-Purified by Immobilised Metal Affinity Chromatography].
- Bacteria used for extraction of gold "freeing": [http://www.goldfields.co.za/company_technology.asp?navDisplay=Company Called BIOX Process]
Chemotaxis Control (E. Coli Filter and E. Coli Rugby)
- Goal: Make E. coli follow/flee some chemical attractant/repellent.
- Questions:
- How does the chemotactic system work?
- Notes:
- Read [http://pubs.acs.org/cgi-bin/sample.cgi/jacsat/2007/129/i21/pdf/ja0692480.pdf Guiding Bacteria with Small Molecules and RNA].
Mechanical Stress Detector
- Goal: Make E. coli respond to mechanical stress.
- Questions:
- How does [http://en.wikipedia.org/wiki/Mechanotransduction mechanotransduction] work in E. coli?
- Notes:
- Sound waves are mechanical stress, so this is an acoustic sensor. We would have to consider some frequency range.
- Read [http://www.childrenshospital.org/research/ingber/PDF/2002/STKE-Alenghat.pdf Mechanotransduction: All Signals Point to Cytoskeleton, Matrix, and Integrins] and [http://www.proteinscience.org/cgi/content/abstract/8/10/1915 Structure and Function of the Bacterial Mechanosensitive Channel of Large Conductance].
Lipopolysaccharide Detector
- Goal: Make E. coli detect lipopolysaccharide (LPS).
- Notes:
- LPS leads to inflammation in higher organisms.
Light Sensor (E. Coli Sunscreen)
- Goal: Make E. coli respond to varying intensities of light.
