Team:The University of Alberta/29 May 2008

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(→On this Episode of "What we Did in the Lab Today")
(→On this Episode of "What we Did in the Lab Today")
 
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==On this Episode of "What we Did in the Lab Today"==
==On this Episode of "What we Did in the Lab Today"==
'''Chris''':
'''Chris''':
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* Got sequencing results on B0043 back. Right now, the sequence files for only the first two genes in the operon have been put in the IGEM folder so I can only check to see if those two are right:
+
* Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason):
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** B0043+1 (the first gene) seems to be Enoyl-CoA Hydratase
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** B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1)
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** B0043+2 (the second gene) seems to be Butyryl-CoA Dehydrogenase
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** B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase
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Are these in the correct order? (James thinks theyre supposed to be the other way around). I dont know much about last year's project so I can't say.<br>
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** B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase
 +
** B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase
 +
** B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase
 +
** B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon
 +
** B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far.
 +
I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)<br>
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'''Jason'''
'''Jason'''
* Researched the GAA promoter that we could used for our ERE.
* Researched the GAA promoter that we could used for our ERE.
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*Learned how to run a protien gel
*Learned how to run a protien gel
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'''Tune in Later for the Exciting conclusion of "What we did in the lab today"
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'''Tune in Later for the Exciting conclusion of "What we did in the lab today"'''

Latest revision as of 18:49, 29 May 2008

Volunteers Scheduled for Today

130-4 NK
4-8 AH
5-9 KR
6-9 DL

On this Episode of "What we Did in the Lab Today"

Chris:

  • Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason):
    • B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1)
    • B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase
    • B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase
    • B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase
    • B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase
    • B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon
    • B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far.

I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)

Jason

  • Researched the GAA promoter that we could used for our ERE.
    • Designed a Possible BioBrick
  • Learned how to run a protien gel

Tune in Later for the Exciting conclusion of "What we did in the lab today"

Retrieved from "http://2008.igem.org/Team:The_University_of_Alberta/29_May_2008"