Wisconsin:Lignin Project/4 August 2008
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- | |'''Team Fungus:'''<br> | + | |'''Team Sorbitol:'''<br> |
+ | Began PCR reactions to clone srlD with restriction sites to insert it into the pET28a vector as well as to make it brickable. The PCR products were verified via electrophoresis and showed the correct sizes. | ||
+ | |||
+ | '''Team Fungus:'''<br> | ||
Took 1 ml from all 11 overnight cultures and centrifuged them for 10 min @ 3400 rpm.<br> | Took 1 ml from all 11 overnight cultures and centrifuged them for 10 min @ 3400 rpm.<br> | ||
Purified pEt28a using QIAquick Mini Prep kit.<br> | Purified pEt28a using QIAquick Mini Prep kit.<br> |
Latest revision as of 16:38, 28 October 2008
Team Sorbitol: Began PCR reactions to clone srlD with restriction sites to insert it into the pET28a vector as well as to make it brickable. The PCR products were verified via electrophoresis and showed the correct sizes. Team Fungus: |