Team:University of Ottawa/25 July 2008

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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Welcome_to_the_uOttawa_Team_Wiki.21">Welcome</a></li>
         <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Welcome_to_the_uOttawa_Team_Wiki.21">Welcome</a></li>
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        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Announcements">Announcements</a></li>
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   <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa/Team">The Team</a>
   <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa/Team">The Team</a>
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   <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Project">The Project</a>
   <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Project">The Project</a>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li>
         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Pulsate_Gene_Expression">Expression</a></li>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Cell-to-Cell_Communication">Communication</a></li>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li>
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        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Oscillatory_Dynamics">Oscillation</a></li>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li>
         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Journal_Club">Journal Club</a></li>
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         <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li>
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   <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Parts">BioBricks</a></li>
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   <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li>
   <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li>
   <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li>
   <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a>
   <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a>
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'''Dan'''
'''Dan'''
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:'''PCR of 0A and 0B'''
:'''PCR of 0A and 0B'''
::<li> PCR of constructs 0A and 0B was performed, producing lots of DNA.
::<li> PCR of constructs 0A and 0B was performed, producing lots of DNA.
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Latest revision as of 23:48, 28 October 2008

Untitled Document

 

 


Dan

Miniprep
  • Minipreped the 6 colonies, obatined good concentrations however gel confirmation revealed that transformation was unsuccessful.
  • Gel Extraction
  • Performed gel extraction of T123 amplification product from July 24
  • To much DNA was inserted into each well, will have to do PCR again
  • PCR of 0A and 0B
  • PCR of constructs 0A and 0B was performed, producing lots of DNA.