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Virginia/13 August 2008
From 2008.igem.org
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*Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation | *Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation | ||
*Cooled off butanolysis apparatus, will do extraction soon | *Cooled off butanolysis apparatus, will do extraction soon | ||
| + | **Extracted with 3ml MilliQ water. Saved top organic layer in vials labeled 'butanolysis product' | ||
*Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen) | *Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen) | ||
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[[Team:Virginia/Notebook|Back to Notebook]] | [[Team:Virginia/Notebook|Back to Notebook]] | ||
Latest revision as of 19:45, 13 August 2008
- Made small broth of A58-A60
- Not sure about A58 and A59 - few colonies, satellites
- Made small broth of re-streaked B0034
- Still not confident in this
- Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation
- Cooled off butanolysis apparatus, will do extraction soon
- Extracted with 3ml MilliQ water. Saved top organic layer in vials labeled 'butanolysis product'
- Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen)
