Alberta NINT/29 May 2008

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lab work (SD): Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09).  DNA was run on 2% agarose gel.  All 9 samples were identical on the gel.  K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random and inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
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[[Team:Alberta_NINT/Notebook | < Back to notebook]]
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lab work (JD): Gel purified R0011 band from gel electrophoresis. Carried out quantitative analysis and carried out ligation to create K102001 (R0011+T/A1), transformed XL1-B cells with K102001. Prepared R0011 for sequencing and dropped it off.
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[[Alberta_NINT/28_May_2008 | < Previous entry]] | [[Alberta_NINT/30_May_2008 | Next entry >]]
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lab work (SD):
 +
 
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          Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B)
 +
          (plates from 28/05/09). 
 +
          DNA was run on 2% agarose gel.  All 9 samples were identical on the gel.
 +
          K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were     
 +
          selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
 +
          [[Image:NINT_SD_p19.jpg|500px]]
 +
 
 +
lab work (JD):  
 +
 
 +
          Gel purified R0011 band from gel electrophoresis.  
 +
          Carried out quantitative analysis
 +
          Carried out ligation to create K102001 (R0011+T/A1)
 +
          Transformed XL1-B cells with K102001.  
 +
          Prepared R0011 for sequencing and dropped it off.
 +
          [[Image:slide1.jpg|750px]]
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lab work (WM):
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          # colonies from K102010 ligation: ~100        negative ctl: 0
 +
          setup 8 O/Ns

Latest revision as of 22:05, 11 October 2008

< Back to notebook

< Previous entry | Next entry >


lab work (SD): 

          Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B)
          (plates from 28/05/09).  
          DNA was run on 2% agarose gel.  All 9 samples were identical on the gel. 
          K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were      
          selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
         NINT SD p19.jpg

lab work (JD): 

         Gel purified R0011 band from gel electrophoresis. 
         Carried out quantitative analysis  
         Carried out ligation to create K102001 (R0011+T/A1)
         Transformed XL1-B cells with K102001. 
         Prepared R0011 for sequencing and dropped it off.
         Slide1.jpg

lab work (WM): 

         # colonies from K102010 ligation: ~100        negative ctl: 0
         setup 8 O/Ns
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