Team:Chiba/Calendar-Home/17 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: ===Toggle swich team=== バイオブリックパーツおこし BBa_I73002 BBa_I73004 BBa_I73003 BBa_I763026 Elution :EB buffer 5μL -> Spin(15000rpm,3Min.) Insert PCR <br>DNA template ...)
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===Toggle swich team===
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>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
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バイオブリックパーツおこし
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BBa_I73002
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BBa_I73004
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BBa_I73003
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BBa_I763026
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Elution :EB buffer 5μL -> Spin(15000rpm,3Min.)
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[[Team:Chiba/Calendar-Home/16 August 2008|16 August 2008 <]]|[[Team:Chiba/Calendar-Home/18 August 2008|> 18 August 2008]]
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Insert PCR
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==Laboratory work==
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===Team:Input===
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no work
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<br>DNA template 1μL
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===Team:Communication===
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<br>Fwd primer(VF II) 5μL
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:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
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<br>Rev primer(VR)   5μL
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:competent cells : XL10G
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<br>Termo pol buffer  10μL
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::*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] --> No colonies on plates
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<br>Vent pol 1μL
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::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] --> No colonies on plates
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<br>dH2O 68μL
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::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] --> No colonies on plates
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<br>==========================
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::*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] --> No colonies on plates
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<br>Total:100μL
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::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] --> No colonies on plates
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::*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] --> No colonies on plates
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===Team:Output===
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'''[[Team:Chiba/protocol/PCR|PCR]]'''
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*[http://partsregistry.org/Part:BBa_E0430 BBa_E0430](1)
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*[http://partsregistry.org/Part:BBa_E0420 BBa_E0420](2)
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*[http://partsregistry.org/Part:BBa_J06702 BBa_J06702](3)
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*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416](4)
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*[http://partsregistry.org/Part:BBa_I730003 BBa_I730003](5)
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*[http://partsregistry.org/Part:BBa_I730004 BBa_I730004](6)
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*[http://partsregistry.org/Part:BBa_I763026 BBa_I763026](7)
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*[http://partsregistry.org/Part:BBa_I763002 BBa_I763002](8)
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95℃(5min.) -> [95℃(30sec.) -> 53℃(1min.) -> 72℃(2min.)]x25cycle -> 72℃(10min.) -> store
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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Gel check
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<td width="257">Sample No.</td>
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<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td>
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</tr>
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<tr>
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<td>DNA template(&mu;L)</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>FW primer(&mu;L)</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>VR primer(&mu;L)</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>dNTPmix(&mu;L)</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>thermo pol buffer(&mu;L)</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>DNA pol(VENT)(&mu;L)</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O(&mu;L)</td>
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<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
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</tr>
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<tr>
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<td>TOTAL(&mu;L)</td>
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<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
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</tr>
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</table>

Latest revision as of 05:11, 30 October 2008

>Home | Notebook

16 August 2008 <|> 18 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

Transformation
competent cells : XL10G
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040(2008)] --> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070(2008)] --> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076(2008)] --> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077(2008)] --> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078(2008)] --> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079(2008)] --> No colonies on plates

Team:Output

PCR

  • [http://partsregistry.org/Part:BBa_E0430 BBa_E0430](1)
  • [http://partsregistry.org/Part:BBa_E0420 BBa_E0420](2)
  • [http://partsregistry.org/Part:BBa_J06702 BBa_J06702](3)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](4)
  • [http://partsregistry.org/Part:BBa_I730003 BBa_I730003](5)
  • [http://partsregistry.org/Part:BBa_I730004 BBa_I730004](6)
  • [http://partsregistry.org/Part:BBa_I763026 BBa_I763026](7)
  • [http://partsregistry.org/Part:BBa_I763002 BBa_I763002](8)
Sample No. 12345678
DNA template(μL) 11111111
FW primer(μL) 55555555
VR primer(μL) 55555555
dNTPmix(μL) 1010101010101010
thermo pol buffer(μL) 1010101010101010
DNA pol(VENT)(μL) 11111111
dH2O(μL) 6868686868686868
TOTAL(μL) 100100100100100100100100
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/17_August_2008"