Edinburgh/7 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
 
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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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<head>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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var tabdropdown={
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:::: '''[[Edinburgh/6_July_2008|< Previous Entry]]'''
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disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
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disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
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enableiframeshim: 1, //1 or 0, for true or false
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//No need to edit beyond here////////////////////////
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== Week 4 ==
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dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
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=== Monday 7 July 08 ===
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currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
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getposOffset:function(what, offsettype){
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* Several more white colonies have grown on plate 19 (pSB1A2+''appY''), but quite small and close together. Tried to sub these to '''Plate 21'''.
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var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
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* Nimisha and the team did two maxipreps - '''X1''' (J33201), and '''X2''' (the ''dxs'' clone of miniprep M2). X2 digested with HindIII (single restriction digest).
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var parentEl=what.offsetParent;
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* Purified the mutagenic PCR reactions P6 (BABEL2+''glgC''-mut1) and P7 (BABEL2+''glgC''-mut2), and set up self ligations ('''L6 and L7''').
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while (parentEl!=null){
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* Sequence results for ''glgC'' clones M10 and M11 show that both are in reverse orientation. The result for the ''dxs'' clones M2 looks OK at first glance, but can't see restriction sites since sequencing primers pSB1A2insf1 and pSB1A2insr1 were used rather than pSB1A2f2 and pSB1A2r2.<br />
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totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop;
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<br />
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parentEl=parentEl.offsetParent;
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}
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return totaloffset;
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},
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showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
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:::: '''[[Edinburgh/8_July_2008|Next Entry >]]'''
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if (this.ie || this.firefox)
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this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
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-
if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){
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if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one
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obj2.parentNode.className="selected"
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obj.visibility="visible"
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}
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else if (e.type=="click")
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obj.visibility="hidden"
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},
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iecompattest:function(){
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return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
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},
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clearbrowseredge:function(obj, whichedge){
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var edgeoffset=0
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if (whichedge=="rightedge"){
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var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
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if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure)  //move menu to the left?
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edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth
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}
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else{
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var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset
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var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
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-
if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up?
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edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight
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if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either?
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edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge
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}
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this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
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}
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return edgeoffset
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},
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dropit:function(obj, e, dropmenuID){
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if (this.dropmenuobj!=null){ //hide previous menu
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this.dropmenuobj.style.visibility="hidden" //hide menu
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if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
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if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
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this.previousmenuitem.parentNode.className=""
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}
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}
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this.clearhidemenu()
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if (this.ie||this.firefox){
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obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
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obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
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this.dropmenuobj=document.getElementById(dropmenuID)
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this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
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this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
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this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
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this.showhide(this.dropmenuobj.style, e, obj)
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this.dropmenuobj.x=this.getposOffset(obj, "left")
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-
this.dropmenuobj.y=this.getposOffset(obj, "top")
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-
this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
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-
this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
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this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
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this.positionshim() //call iframe shim function
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-
}
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},
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contains_firefox:function(a, b) {
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while (b.parentNode)
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if ((b = b.parentNode) == a)
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-
return true;
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return false;
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},
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dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
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var evtobj=window.event? window.event : e
+
-
if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
+
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this.delayhidemenu(obj2)
+
-
else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
+
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this.delayhidemenu(obj2)
+
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},
+
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+
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delayhidemenu:function(obj2){
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this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
+
-
},
+
-
 
+
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clearhidemenu:function(){
+
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if (this.delayhide!="undefined")
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clearTimeout(this.delayhide)
+
-
},
+
-
 
+
-
positionshim:function(){ //display iframe shim function
+
-
if (this.enableiframeshim && typeof this.shimobject!="undefined"){
+
-
if (this.dropmenuobj.style.visibility=="visible"){
+
-
this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
+
-
this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
+
-
this.shimobject.style.left=this.dropmenuobj.style.left
+
-
this.shimobject.style.top=this.dropmenuobj.style.top
+
-
}
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-
this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
+
-
}
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-
},
+
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+
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hideshim:function(){
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if (this.enableiframeshim && typeof this.shimobject!="undefined")
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this.shimobject.style.display='none'
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-
},
+
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+
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isSelected:function(menuurl){
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var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
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-
return (tabdropdown.currentpageurl==menuurl)
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},
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init:function(menuid, dselected){
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this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
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var menuitems=document.getElementById(menuid).getElementsByTagName("a")
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-
for (var i=0; i<menuitems.length; i++){
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if (menuitems[i].getAttribute("rel")){
+
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var relvalue=menuitems[i].getAttribute("rel")
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-
document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
+
-
menuitems[i].onmouseover=function(e){
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var event=typeof e!="undefined"? e : window.event
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tabdropdown.dropit(this, event, this.getAttribute("rel"))
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-
}
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-
}
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if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
+
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menuitems[i].parentNode.className+=" selected default"
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-
var setalready=true
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-
}
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else if (parseInt(dselected)==i)
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menuitems[i].parentNode.className+=" selected default"
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-
}
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-
}
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-
 
+
-
}
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</script>
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<style>
+
-
.ddcolortabs{
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padding: 0;
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width: 100%;
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-
background: transparent;
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voice-family: "\"}\"";
+
-
voice-family: inherit;
+
-
}
+
-
 
+
-
.ddcolortabs ul{
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font: normal 11px Arial, Verdana, sans-serif;
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-
margin:0;
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padding:0;
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list-style:none;
+
-
}
+
-
 
+
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.ddcolortabs li{
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display:inline;
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margin:0 2px 0 0;
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padding:0;
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text-transform:uppercase;
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}
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+
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.ddcolortabs a{
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float:left;
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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padding:0 0 1px 3px;
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text-decoration:none;
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letter-spacing: 1px;
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}
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+
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.ddcolortabs a span{
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float:left;
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display:block;
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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padding: 4px 8px 2px 7px;
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-
}
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-
 
+
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.ddcolortabs a span{
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float:none;
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-
}
+
-
 
+
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.ddcolortabs a:hover{
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-
background-color: #591f20;
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-
}
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-
 
+
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.ddcolortabs a:hover span{
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-
background-color: #591f20;
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-
}
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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background-color: #591f20;
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-
}
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.ddcolortabsline{
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clear: both;
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-
padding: 0;
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width: 100%;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
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border-bottom-width: 0;
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font:normal 12px Arial;
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line-height:18px;
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z-index:100;
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-
background-color: white;
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-
width: 200px;
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-
visibility: hidden;
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-
}
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-
 
+
-
 
+
-
.dropmenudiv_a a{
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-
width: auto;
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-
display: block;
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-
text-indent: 5px;
+
-
border-top: 0 solid #678b3f;
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-
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
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padding: 2px 0;
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text-decoration: none;
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color: black;
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-
}
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-
 
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* html .dropmenudiv_a a{ /*IE only hack*/
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-
width: 100%;
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-
}
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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-
background-color: #8a3c3d;
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color: white;
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-
}
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-
</style>
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-
</head>
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<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
+
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
+
-
</ul>
+
-
</div>
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-
<div class="ddcolortabsline">&nbsp;</div>
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-
<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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</div>
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-
<script type="text/javascript">
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//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
+
-
tabdropdown.init("colortab", 3)
+
-
</script>
+
-
</html>
+
-
 
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=== Week 4 ===
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-
==== Monday 7 July 08 ====
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-
 
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* Several more white colonies have grown on plate 19, but quite small and close together. Tried to sub these to '''Plate 21'''.
+
-
* Nimisha and the team did two maxipreps - '''X1''' (J33201), and '''X2''' (the ''dxs'' clone of miniprep M2). X2 digested with HindIII (single restriction digest).
+
-
* Purified the mutagenic PCR reactions P6 and P7, and set up self ligations ('''L6 and L7''').
+
-
* Sequence results for ''glgC'' clones M10 and M11 show that both are in reverse orientation. The result for the ''dxs'' clones M2 looks OK at first glance, but can't see restriction sites since sequencing primers pSB1A2insf1 and pSB1A2insr1 were used rather than pSB1A2f2 and pSB1A2r2.
+

Latest revision as of 14:06, 28 August 2008

< Previous Entry

Week 4

Monday 7 July 08

  • Several more white colonies have grown on plate 19 (pSB1A2+appY), but quite small and close together. Tried to sub these to Plate 21.
  • Nimisha and the team did two maxipreps - X1 (J33201), and X2 (the dxs clone of miniprep M2). X2 digested with HindIII (single restriction digest).
  • Purified the mutagenic PCR reactions P6 (BABEL2+glgC-mut1) and P7 (BABEL2+glgC-mut2), and set up self ligations (L6 and L7).
  • Sequence results for glgC clones M10 and M11 show that both are in reverse orientation. The result for the dxs clones M2 looks OK at first glance, but can't see restriction sites since sequencing primers pSB1A2insf1 and pSB1A2insr1 were used rather than pSB1A2f2 and pSB1A2r2.


Next Entry >
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