Edinburgh/8 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
 
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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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<head>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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var tabdropdown={
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:::: '''[[Edinburgh/7_July_2008|< Previous Entry]]'''
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disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
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disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
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enableiframeshim: 1, //1 or 0, for true or false
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//No need to edit beyond here////////////////////////
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== Week 4 ==
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dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
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=== Tuesday 8 July 08 ===
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currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
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* New primers for ''crt'' BioBricks arrived. Performed PCR with KOD using primer pairs crtBf2 (new) + crtBr2 (old)('''P8'''), crtIf2 + crtIr2 (both new) ('''P9'''), and crtYf2 (new) + crtYr2 (old)('''P10'''). The template for P8 and P10 was a suspension of ''Pantoea ananatis'' cells from plate 17; for P9, Douglas's maxiprep DNA from 19 Feb (with the PstI sites mutated out) was used. In all cases annealing was at 55°C and extension for 30s. '''Gel 9''' showed that P8 and P9 had worked, with pure products of the expected size seen, but no product was seen for P10.
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var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
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* ''glgC'' mutagenesis: Transformed competent cells with ligation products L6 and L7; plated cells on '''Plates 22 and 23''' (L6: BABEL2+''glgC''-mut1) and '''Plates 24 and 25''' (L7: BABEL2+''glgC''-mut2).
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var parentEl=what.offsetParent;
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* Ran '''gel 9''' to check size of X1 (J33201) and X2 (pSB1A2+''dxs'') HindIII restriction digest. The digest looked fine.<br />
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parentEl=parentEl.offsetParent;
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return totaloffset;
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},
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showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
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:::: '''[[Edinburgh/9_July_2008|Next Entry >]]'''
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this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
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obj2.parentNode.className="selected"
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obj.visibility="visible"
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
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this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
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dropit:function(obj, e, dropmenuID){
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if (this.dropmenuobj!=null){ //hide previous menu
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this.dropmenuobj.style.visibility="hidden" //hide menu
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if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
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if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
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this.previousmenuitem.parentNode.className=""
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this.clearhidemenu()
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if (this.ie||this.firefox){
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obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
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obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
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this.dropmenuobj=document.getElementById(dropmenuID)
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this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
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this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
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this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
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this.showhide(this.dropmenuobj.style, e, obj)
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this.dropmenuobj.x=this.getposOffset(obj, "left")
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this.dropmenuobj.y=this.getposOffset(obj, "top")
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this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
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this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
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this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
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this.positionshim() //call iframe shim function
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contains_firefox:function(a, b) {
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while (b.parentNode)
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dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
+
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var evtobj=window.event? window.event : e
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if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
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this.delayhidemenu(obj2)
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else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
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this.delayhidemenu(obj2)
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},
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delayhidemenu:function(obj2){
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this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
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},
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+
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clearhidemenu:function(){
+
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if (this.delayhide!="undefined")
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clearTimeout(this.delayhide)
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},
+
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positionshim:function(){ //display iframe shim function
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if (this.dropmenuobj.style.visibility=="visible"){
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this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
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this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
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this.shimobject.style.left=this.dropmenuobj.style.left
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this.shimobject.style.top=this.dropmenuobj.style.top
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}
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this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
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}
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},
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+
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hideshim:function(){
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if (this.enableiframeshim && typeof this.shimobject!="undefined")
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this.shimobject.style.display='none'
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},
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+
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isSelected:function(menuurl){
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var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
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return (tabdropdown.currentpageurl==menuurl)
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},
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init:function(menuid, dselected){
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this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
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var menuitems=document.getElementById(menuid).getElementsByTagName("a")
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-
for (var i=0; i<menuitems.length; i++){
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-
if (menuitems[i].getAttribute("rel")){
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var relvalue=menuitems[i].getAttribute("rel")
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-
document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
+
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menuitems[i].onmouseover=function(e){
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-
var event=typeof e!="undefined"? e : window.event
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tabdropdown.dropit(this, event, this.getAttribute("rel"))
+
-
}
+
-
}
+
-
if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
+
-
menuitems[i].parentNode.className+=" selected default"
+
-
var setalready=true
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}
+
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else if (parseInt(dselected)==i)
+
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menuitems[i].parentNode.className+=" selected default"
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-
}
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-
}
+
-
 
+
-
}
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</script>
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<style>
+
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.ddcolortabs{
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-
padding: 0;
+
-
width: 100%;
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-
background: transparent;
+
-
voice-family: "\"}\"";
+
-
voice-family: inherit;
+
-
}
+
-
 
+
-
.ddcolortabs ul{
+
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font: normal 11px Arial, Verdana, sans-serif;
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margin:0;
+
-
padding:0;
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list-style:none;
+
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}
+
-
 
+
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.ddcolortabs li{
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display:inline;
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margin:0 2px 0 0;
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padding:0;
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text-transform:uppercase;
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}
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+
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.ddcolortabs a{
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float:left;
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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padding:0 0 1px 3px;
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text-decoration:none;
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letter-spacing: 1px;
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}
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+
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.ddcolortabs a span{
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float:left;
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display:block;
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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padding: 4px 8px 2px 7px;
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}
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.ddcolortabs a span{
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float:none;
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}
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.ddcolortabs a:hover{
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background-color: #591f20;
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}
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.ddcolortabs a:hover span{
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background-color: #591f20;
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}
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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background-color: #591f20;
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}
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.ddcolortabsline{
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clear: both;
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padding: 0;
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width: 100%;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
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border-bottom-width: 0;
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font:normal 12px Arial;
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line-height:18px;
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z-index:100;
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-
background-color: white;
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-
width: 200px;
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-
visibility: hidden;
+
-
}
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-
 
+
-
 
+
-
.dropmenudiv_a a{
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-
width: auto;
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-
display: block;
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-
text-indent: 5px;
+
-
border-top: 0 solid #678b3f;
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-
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
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padding: 2px 0;
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text-decoration: none;
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color: black;
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}
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* html .dropmenudiv_a a{ /*IE only hack*/
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width: 100%;
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-
}
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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background-color: #8a3c3d;
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color: white;
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-
}
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-
</style>
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-
</head>
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-
 
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<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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</ul>
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</div>
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<div class="ddcolortabsline">&nbsp;</div>
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-
<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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-
</div>
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-
<script type="text/javascript">
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-
//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
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-
tabdropdown.init("colortab", 3)
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-
</script>
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-
</html>
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-
 
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=== Week 4 ===
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==== Tuesday 8 July 08 ====
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-
 
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* New primers for ''crt'' BioBricks arrived. Performed PCR with KOD using primer pairs crtBf2 (new) + crtBr2 (old)('''P8'''), crtIf2 + crtIr2 (both new) ('''P9'''), and crtYf2 (new) + crtYr2 (old)('''P10'''). The template for P8 and P10 was a suspension of ''Pantoea ananatis'' cells from plate 17; for P9, Douglas's maxiprep DNA from 19 Feb (with the PstI sites mutated out) was used. In all cases annealing was at 55 C and extension for 30s. '''Gel 9''' showed that P8 and P9 had worked, with pure products of the expected size seen, but no product was seen for P10.
+
-
* ''glgC'' mutagenesis: Transformed competent cells with ligation products L6 and L7; plated cells on '''Plates 22 and 23''' (L6) and '''Plates 24 and 25''' (L7).
+
-
* ''dxs'': Ran gel (Gel 9) to check size of X1 (J33201) and X2 (pSB1A2+''dxs'') HindIII restriction digest. The digest looked fine.
+

Latest revision as of 14:06, 28 August 2008

< Previous Entry

Week 4

Tuesday 8 July 08

  • New primers for crt BioBricks arrived. Performed PCR with KOD using primer pairs crtBf2 (new) + crtBr2 (old)(P8), crtIf2 + crtIr2 (both new) (P9), and crtYf2 (new) + crtYr2 (old)(P10). The template for P8 and P10 was a suspension of Pantoea ananatis cells from plate 17; for P9, Douglas's maxiprep DNA from 19 Feb (with the PstI sites mutated out) was used. In all cases annealing was at 55°C and extension for 30s. Gel 9 showed that P8 and P9 had worked, with pure products of the expected size seen, but no product was seen for P10.
  • glgC mutagenesis: Transformed competent cells with ligation products L6 and L7; plated cells on Plates 22 and 23 (L6: BABEL2+glgC-mut1) and Plates 24 and 25 (L7: BABEL2+glgC-mut2).
  • Ran gel 9 to check size of X1 (J33201) and X2 (pSB1A2+dxs) HindIII restriction digest. The digest looked fine.


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