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- | <html> | + | <div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div> |
- | <head>
| + | |
- | <title>Edinburgh iGem 2008</title>
| + | |
- | <script type="text/javascript">
| + | |
- | //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
| + | |
- | //Created: May 16th, 07'
| + | |
- | | + | |
- | var tabdropdown={
| + | |
- | * disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
| + | |
- | disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
| + | |
- | enableiframeshim: 1, //1 or 0, for true or false
| + | |
- | | + | |
- | //No need to edit beyond here////////////////////////
| + | |
- | dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
| + | |
- | currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
| + | |
- | | + | |
- | getposOffset:function(what, offsettype){
| + | |
- | var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
| + | |
- | var parentEl=what.offsetParent;
| + | |
- | while (parentEl!=null){
| + | |
- | totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop;
| + | |
- | parentEl=parentEl.offsetParent;
| + | |
- | }
| + | |
- | return totaloffset;
| + | |
- | },
| + | |
- | | + | |
- | showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
| + | |
- | if (this.ie || this.firefox)
| + | |
- | this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
| + | |
- | if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){
| + | |
- | if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one
| + | |
- | obj2.parentNode.className="selected"
| + | |
- | obj.visibility="visible"
| + | |
- | }
| + | |
- | else if (e.type=="click")
| + | |
- | obj.visibility="hidden"
| + | |
- | },
| + | |
- | | + | |
- | iecompattest:function(){
| + | |
- | return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
| + | |
- | },
| + | |
- | | + | |
- | clearbrowseredge:function(obj, whichedge){
| + | |
- | var edgeoffset=0
| + | |
- | if (whichedge=="rightedge"){
| + | |
- | var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15
| + | |
- | this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
| + | |
- | if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure) //move menu to the left?
| + | |
- | edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth
| + | |
- | }
| + | |
- | else{
| + | |
- | var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset
| + | |
- | var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18
| + | |
- | this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
| + | |
- | if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up?
| + | |
- | edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight
| + | |
- | if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either?
| + | |
- | edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge
| + | |
- | }
| + | |
- | this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
| + | |
- | }
| + | |
- | return edgeoffset
| + | |
- | },
| + | |
- | | + | |
- | dropit:function(obj, e, dropmenuID){
| + | |
- | if (this.dropmenuobj!=null){ //hide previous menu
| + | |
- | this.dropmenuobj.style.visibility="hidden" //hide menu
| + | |
- | if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
| + | |
- | if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
| + | |
- | this.previousmenuitem.parentNode.className=""
| + | |
- | }
| + | |
- | }
| + | |
- | this.clearhidemenu()
| + | |
- | if (this.ie||this.firefox){
| + | |
- | obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
| + | |
- | obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
| + | |
- | this.dropmenuobj=document.getElementById(dropmenuID)
| + | |
- | this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
| + | |
- | this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
| + | |
- | this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
| + | |
- | this.showhide(this.dropmenuobj.style, e, obj)
| + | |
- | this.dropmenuobj.x=this.getposOffset(obj, "left")
| + | |
- | this.dropmenuobj.y=this.getposOffset(obj, "top")
| + | |
- | this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
| + | |
- | this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
| + | |
- | this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
| + | |
- | this.positionshim() //call iframe shim function
| + | |
- | }
| + | |
- | },
| + | |
- | | + | |
- | contains_firefox:function(a, b) {
| + | |
- | while (b.parentNode)
| + | |
- | if ((b = b.parentNode) == a)
| + | |
- | return true;
| + | |
- | return false;
| + | |
- | },
| + | |
- | | + | |
- | dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
| + | |
- | var evtobj=window.event? window.event : e
| + | |
- | if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
| + | |
- | this.delayhidemenu(obj2)
| + | |
- | else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
| + | |
- | this.delayhidemenu(obj2)
| + | |
- | },
| + | |
- | | + | |
- | delayhidemenu:function(obj2){
| + | |
- | this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
| + | |
- | },
| + | |
- | | + | |
- | clearhidemenu:function(){
| + | |
- | if (this.delayhide!="undefined")
| + | |
- | clearTimeout(this.delayhide)
| + | |
- | },
| + | |
- | | + | |
- | positionshim:function(){ //display iframe shim function
| + | |
- | if (this.enableiframeshim && typeof this.shimobject!="undefined"){
| + | |
- | if (this.dropmenuobj.style.visibility=="visible"){
| + | |
- | this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
| + | |
- | this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
| + | |
- | this.shimobject.style.left=this.dropmenuobj.style.left
| + | |
- | this.shimobject.style.top=this.dropmenuobj.style.top
| + | |
- | }
| + | |
- | this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
| + | |
- | }
| + | |
- | },
| + | |
- | | + | |
- | hideshim:function(){
| + | |
- | if (this.enableiframeshim && typeof this.shimobject!="undefined")
| + | |
- | this.shimobject.style.display='none'
| + | |
- | },
| + | |
- | | + | |
- | isSelected:function(menuurl){
| + | |
- | var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
| + | |
- | return (tabdropdown.currentpageurl==menuurl)
| + | |
- | },
| + | |
- | | + | |
- | init:function(menuid, dselected){
| + | |
- | this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
| + | |
- | var menuitems=document.getElementById(menuid).getElementsByTagName("a")
| + | |
- | for (var i=0; i<menuitems.length; i++){
| + | |
- | if (menuitems[i].getAttribute("rel")){
| + | |
- | var relvalue=menuitems[i].getAttribute("rel")
| + | |
- | document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
| + | |
- | menuitems[i].onmouseover=function(e){
| + | |
- | var event=typeof e!="undefined"? e : window.event
| + | |
- | tabdropdown.dropit(this, event, this.getAttribute("rel"))
| + | |
- | }
| + | |
- | }
| + | |
- | if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
| + | |
- | menuitems[i].parentNode.className+=" selected default"
| + | |
- | var setalready=true
| + | |
- | }
| + | |
- | else if (parseInt(dselected)==i)
| + | |
- | menuitems[i].parentNode.className+=" selected default"
| + | |
- | }
| + | |
- | }
| + | |
- | | + | |
- | }
| + | |
- | </script>
| + | |
- | <style>
| + | |
- | .ddcolortabs{
| + | |
- | padding: 0;
| + | |
- | width: 100%;
| + | |
- | background: transparent;
| + | |
- | voice-family: "\"}\"";
| + | |
- | voice-family: inherit;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabs ul{
| + | |
- | font: normal 11px Arial, Verdana, sans-serif;
| + | |
- | margin:0;
| + | |
- | padding:0;
| + | |
- | list-style:none;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabs li{
| + | |
- | display:inline;
| + | |
- | margin:0 2px 0 0;
| + | |
- | padding:0;
| + | |
- | text-transform:uppercase;
| + | |
- | }
| + | |
- | | + | |
- | | + | |
- | .ddcolortabs a{
| + | |
- | float:left;
| + | |
- | color: white;
| + | |
- | background: black url(media/color_tabs_left.gif) no-repeat left top;
| + | |
- | margin:0 2px 0 0;
| + | |
- | padding:0 0 1px 3px;
| + | |
- | text-decoration:none;
| + | |
- | letter-spacing: 1px;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabs a span{
| + | |
- | float:left;
| + | |
- | display:block;
| + | |
- | background: transparent url(media/color_tabs_right.gif) no-repeat right top;
| + | |
- | padding: 4px 8px 2px 7px;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabs a span{
| + | |
- | float:none;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabs a:hover{
| + | |
- | background-color: #591f20;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabs a:hover span{
| + | |
- | background-color: #591f20;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
| + | |
- | background-color: #591f20;
| + | |
- | }
| + | |
- | | + | |
- | .ddcolortabsline{
| + | |
- | clear: both;
| + | |
- | padding: 0;
| + | |
- | width: 100%;
| + | |
- | height: 8px;
| + | |
- | line-height: 8px;
| + | |
- | background: black;
| + | |
- | border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
| + | |
- | }
| + | |
- | | + | |
- | /* ######### Style for Drop Down Menu ######### */
| + | |
- | | + | |
- | .dropmenudiv_a{
| + | |
- | position:absolute;
| + | |
- | top: 0;
| + | |
- | border: 1px solid black; /*THEME CHANGE HERE*/
| + | |
- | border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
| + | |
- | border-bottom-width: 0;
| + | |
- | font:normal 12px Arial;
| + | |
- | line-height:18px;
| + | |
- | z-index:100;
| + | |
- | background-color: white;
| + | |
- | width: 200px;
| + | |
- | visibility: hidden;
| + | |
- | }
| + | |
- | | + | |
- | | + | |
- | .dropmenudiv_a a{
| + | |
- | width: auto;
| + | |
- | display: block;
| + | |
- | text-indent: 5px;
| + | |
- | border-top: 0 solid #678b3f;
| + | |
- | border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
| + | |
- | padding: 2px 0;
| + | |
- | text-decoration: none;
| + | |
- | color: black;
| + | |
- | }
| + | |
- | | + | |
- | * html .dropmenudiv_a a{ /*IE only hack*/
| + | |
- | width: 100%;
| + | |
- | }
| + | |
- | | + | |
- | .dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
| + | |
- | background-color: #8a3c3d;
| + | |
- | color: white;
| + | |
- | }
| + | |
- | </style>
| + | |
- | </head>
| + | |
- | | + | |
- | <!-- CSS for Drop Down Tabs Menu #1 -->
| + | |
- | <link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
| + | |
- | | + | |
- | <div id="colortab" class="ddcolortabs">
| + | |
- | <ul>
| + | |
- | <li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
| + | |
- | <li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
| + | |
- | <li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
| + | |
- | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
| + | |
- | <li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
| + | |
- | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
| + | |
- | </ul>
| + | |
- | </div>
| + | |
- | <div class="ddcolortabsline"> </div>
| + | |
- | <div id="dropmenu1_a" class="dropmenudiv_a">
| + | |
- | <a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
| + | |
- | <a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
| + | |
- | <a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
| + | |
- | </div>
| + | |
- | <script type="text/javascript">
| + | |
- | //SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
| + | |
- | tabdropdown.init("colortab", 3)
| + | |
- | </script>
| + | |
- | </html>
| + | |
| | | |
- | For more information, please see [https://www.wiki.ed.ac.uk/display/iGEM2008/HOME our internal wiki].
| + | == Calendar == |
| | | |
- | == Calender ==
| + | Click on a date to see a summary of labwork done on that day. Up-to-date to end 12 October. Best viewed with Mozilla Firefox. |
| | | |
| <table> | | <table> |
Line 323: |
Line 35: |
| {| align="center" | | {| align="center" |
| |-valign="top" | | |-valign="top" |
- | |align="center" width="200pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | + | |align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} |
- | |align="center" width="200pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | + | |align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} |
- | |align="center" width="200pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | + | |align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} |
| + | |align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=09}} |
| + | |align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=10}} |
| |} | | |} |
| | | |
- | == Summary of samples: == | + | == Summary of samples == |
- | | + | |
- | [PCR products]
| + | |
- | | + | |
- | '''P1:''' ''dxs'' from ''Eschaerichia coli'' JM109 (or K12?)<br />
| + | |
- | '''P2:''' ''appY'' from ''E. coli'' JM109 (or K12?)<br />
| + | |
- | '''P3:''' ''glgC'' from ''E. coli'' JM109 (or K12?)<br />
| + | |
- | '''P4:''' fusion PCR for BABEL1+''glgC''<br />
| + | |
- | '''P5:''' fusion PCR for BABEL2+''glgC''<br />
| + | |
- | '''P6:''' mutagenic PCR to remove EcoRI site 1 from ''glgC''<br />
| + | |
- | '''P7:''' mutagenic PCR to remove EcoRI site 2 from ''glgC''<br />
| + | |
- | '''P8:''' ''crtB'' (from ''Pantoea ananatis'' cells)<br />
| + | |
- | '''P9:''' ''crtI'' (from Douglas's maxiprep of the mutated ''crtIB'' plasmid)<br />
| + | |
- | '''P10:''' ''crtY'' (from ''P. ananatis'' cells). Failed.<br />
| + | |
- | '''P11:''' fusion PCR for rbs+''dxs''. Failed.<br />
| + | |
- | '''P12:''' repeat fusion PCR for rbs+''dxs'', using L11 and primers rbs2fclon1+pSB1A2insr1.<br />
| + | |
- | '''P13 and P14:''' MABEL mutation of site 1 on ''glgC'' mutant clones M19 and M22.<br />
| + | |
- | '''P15:''' fusion PCR for rbs+''appY''<br />
| + | |
- | '''P16, P17, P18 and P19:''' first attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
| + | |
- | '''P20, P21, P22 and P23:''' second attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
| + | |
- | '''P24, P25:''' repeat PCR for ''cenA'' and ''cex'' using Pfu. Failed.<br />
| + | |
- | '''P26:''' Positive control PCR with primers fd1 and rd1 to amplify 16S rRNA gene ''rrnB'' from ''Cellulomonas fimi''.<br />
| + | |
- | '''P27:''' ''crtY'' from ''P. ananatis'' cells. - Failed<br />
| + | |
- | '''P28, P29:''' ''crtB'' and ''crtI'' from L18 and L19.<br />
| + | |
- | '''P30:''' Repeat positive control ''rrnB'' from ''C. fimi'', annealing 1 minute.<br />
| + | |
- | '''P31:''' pZntA promoter<br />
| + | |
- | '''P32-P35:''' repeat PCR for ''cenA'', ''cenB'', ''cenC'' and ''cex'', annealing 1 minute. Failed.<br />
| + | |
- | '''P36:''' M43 (''glgC''-mut1,2) with primers glgCf2/glgCr2 (25.07.08: AM)<br />
| + | |
- | '''P37:''' ''crtY'' from ''P. ananatis''. (25.07.08: AM) - Failed<br />
| + | |
- | '''P38~P41:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from heat-killed cell suspension.<br />
| + | |
- | '''P42~P45:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from 'impure' DNA solution.<br />
| + | |
- | '''P46~P47:''' ''cenA'' and ''cex'' from 'impure' DNA solution, annealing 65C. Failed.<br />
| + | |
- | '''P48:''' ''crtY'' (new primer mixture) (previously P38a) (30.07.08: CF) - Failed<br />
| + | |
- | '''P49:''' Recreation of P12 (rbs+''dxs'') (previously P39a) (30.07.08: CF)<br />
| + | |
- | '''P50:''' third mutagenesis of ''glgc''-mut1,2 (04.08.08: YAN)<br />
| + | |
- | '''P51:''' pCstA from ''E. coli'' cells (06.08.08: CF) - Failed<br />
| + | |
- | '''P52:''' ''cenA'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
| + | |
- | '''P53:''' ''cex'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
| + | |
- | '''P54:''' pCstA from ''E. coli'' cells - Purified (07.08.08: CF)<br />
| + | |
- | | + | |
- | ==== Ligations ====
| + | |
- | | + | |
- | '''L1:''' Edinbrick1+''dxs''<br />
| + | |
- | '''L2:''' Edinbrick1+''appY''<br />
| + | |
- | '''L3:''' Babel1+''glgC''<br />
| + | |
- | '''L4:''' Babel2+''glgC''<br />
| + | |
- | '''L5:''' Edinbrick1+''appY'' repeat (initially labelled L4 in error)<br />
| + | |
- | '''L6:''' Self ligation of mutagenic PCR P6<br />
| + | |
- | '''L7:''' Self ligation of mutagenic PCR P7<br />
| + | |
- | '''L8:''' Ligation of synthetic ribosome binding site to M2 (BBa_K118000)<br />
| + | |
- | '''L9:''' Edinbrick1+''crtB''<br />
| + | |
- | '''L10:''' Edinbrick1+''crtI'' (mutant lacking PstI sites, I hope)<br />
| + | |
- | '''L11:''' repeat ligation of synthetic ribosome binding site to M2 (BBa_K118000)<br />
| + | |
- | '''L12:''' rbs ligated with ''appY''.<br />
| + | |
- | '''L13 and L14:''' self ligations of ''glgC'' mutation reactions P13 and P14.<br />
| + | |
- | '''L15:''' ligation of Edinbrick1 with rbs+''dxs'' fusion PCR product (P12). - Failed<br />
| + | |
- | '''L16:''' Ligation of Edinbrick1 with rbs+''appY'' fusion PCR product (P15) (17/07/08) - Failed<br />
| + | |
- | '''L17:''' Ligation of ''crtB'' (M36) to rbs (22/07/08)<br />
| + | |
- | '''L18:''' Ligation of ''crtI'' (M42) to rbs (22/07/08)<br />
| + | |
- | '''L19:''' pSB1A2+rbs+''dxs'' ligation product (From P12) (25/07/08: Yan/OG)<br />
| + | |
- | '''L20:''' pSB1A2+rbs+''appY'' ligation product (From P15) (25/07/08: Yan/OG)<br />
| + | |
- | '''L21:''' pSB1A2+rbs+''crtB'' ligation product (From P28) (25/07/08: Yan/OG)<br />
| + | |
- | '''L22:''' pSB1A2+rbs+''crtI'' ligation product (From P29) (25/07/08: Yan/OG)<br />
| + | |
- | '''L23:''' PzntA+pSB1A2 ligation product (From P31) (25/07/08: Yan/OG)<br />
| + | |
- | '''L24:''' Ligation of ''crtE'' to pSB1A2 (25/07/08: CF)<br />
| + | |
- | '''L25:''' Ligation of ''glgC'' double mutant (P36) to Edinbrick 1 (28.07.2008: AM)<br />
| + | |
- | '''L26:''' Ligation of Edi+P15 XP - rbs+''appY'' (30.07.2008: HX)<br />
| + | |
- | '''L27:''' Ligation of Edi+P28 XP - rbs+''crtB'' (30.07.2008: HX)<br />
| + | |
- | '''L28:''' Putative ''cenA'' ligated to Edinbrick1 (30.07.08: AM)<br />
| + | |
- | '''L29:''' Putative ''cex'' ligated to Edinbrick1 (30.07.08: AM)<br />
| + | |
- | '''L30:''' Self-ligation of P15 (3rd mutagenesis of ''glgC''-mut1,2) (05.08.08: HX)<br />
| + | |
- | '''L31:''' Ligation of P36 (''glgC''-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)<br />
| + | |
- | '''L32:''' Ligation of rbs+''crtB'' (M67) to rbs+''crtI'' (M50) to create ''crtB''/''crtI'' (07.08.08: AM, Yan)<br />
| + | |
- | '''L33:''' Ligation of PcstA (P54) to Edinbrick1 (08.08.08: OG)<br />
| + | |
- | | + | |
- | ==== Minipreps ====
| + | |
- | | + | |
- | '''M1 to M6:''' pSB1A2+''dxs'' transformants. *M2, M3, M4 and M6* all looked correct on a gel. *M2* was sequenced to confirm identity and was maxiprepped as *X2*.<br />
| + | |
- | '''M7:''' Babel1+''glgC'' transformant. Did not look right on gel.<br />
| + | |
- | '''M8 to M12:''' Babel2+''glgC''_ transformants. *M10 and M11* both looked good on a gel, but sequencing showed that both had the insert in the reverse orientation.<br />
| + | |
- | '''M13 to M18:''' pSB1A2+''appY'' transformants. Gel 10 showed that M15 to M18, and possibly M14, all seemed to have inserts the right size.<br />
| + | |
- | '''M19 to M24:''' Babel2+''glgC'' clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site.<br />
| + | |
- | '''M25 to M30:''' possible rbs+''dxs'' transformants.<br />
| + | |
- | '''M31 to M36:''' ''crtB'' clones.<br />
| + | |
- | '''M37 to M42:''' ''crtI'' clones.<br />
| + | |
- | '''M43 to M47:''' possible ''glgC'' double mutants (both EcoRI sites gone)<br />
| + | |
- | '''M48:''' a possible rbs+''dxs'' clone.<br />
| + | |
- | '''M49 to M54:''' pSB1A2-rbs+''crtI''<br />
| + | |
- | '''M55 to M60:''' PZntA promoter<br />
| + | |
- | '''M61 to M66:''' BABEL2+rbs+''crtE''<br />
| + | |
- | '''M67:''' rbs+''crtB''<br />
| + | |
- | '''M68 to M71:''' pSB1A2+''glgC''-mut1,2 (31.07.08: AM, YAN)<br />
| + | |
- | '''M72 to M75:''' pSB1A2+rbs+''dxs'' (31.07.08: AM, YAN)<br />
| + | |
- | '''M76 to M79:''' BABEL2+''crtE'' (31.07.08: AM, YAN)<br />
| + | |
- | '''M80 to M83:''' pSB1A2+rbs+''appY'' (04/08/08: YAN and OG)<br />
| + | |
- | '''M84 to M85:''' pSB1A2+rbs+''crtB'' (04/08/08: YAN and OG)<br />
| + | |
- | '''M86 to M90:''' pSB1A2+''cenA'' (04/08/08: YAN and OG)<br />
| + | |
- | '''M91 to M96:''' pSB1A2+''cex''(04/08/08: YAN and OG)<br />
| + | |
- | '''M97 to M104:''' pSB1A2+''cenA'' (05.08.08: YAN)<br />
| + | |
- | '''M105 to M108:''' pSB1A2+''cex'' (05.08.08: YAN)<br />
| + | |
| | | |
- | ==== Maxipreps ====
| + | Up-to-date to end 28 October. |
| | | |
- | '''X1:''' BioBrick J33201<br />
| + | [[Edinburgh/Notebook/PCR products|PCR products]]<br /> |
- | '''X2:''' pSB1A2+''dxs'' clone (as M2; BBa_K118000)<br />
| + | [[Edinburgh/Notebook/Ligation products|Ligation products]]<br /> |
- | '''X3:''' pSB1A2+''appY'' (from Plate 21, streak 3) (18 July 2008)<br />
| + | [[Edinburgh/Notebook/Minipreps|Minipreps]]<br /> |
- | '''X4:''' pSB1A2+''crtB'' (as M36; BBa_K118002) (25.07.08: A
| + | [[Edinburgh/Notebook/Maxipreps|Maxipreps]]<br /> |
| + | [[Edinburgh/Notebook/Plates|Plates]]<br /> |
Click on a date to see a summary of labwork done on that day. Up-to-date to end 12 October. Best viewed with Mozilla Firefox.