Team:Edinburgh/Notebook

From 2008.igem.org

(Difference between revisions)
(→Summary of samples:)
 
(24 intermediate revisions not shown)
Line 1: Line 1:
-
<html>
+
<div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div>
-
<head>
+
-
<title>Edinburgh iGem 2008</title>
+
-
<script type="text/javascript">
+
-
//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
+
-
//Created: May 16th, 07'
+
-
 
+
-
var tabdropdown={
+
-
* disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
+
-
disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
+
-
enableiframeshim: 1, //1 or 0, for true or false
+
-
 
+
-
//No need to edit beyond here////////////////////////
+
-
dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
+
-
currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
+
-
 
+
-
getposOffset:function(what, offsettype){
+
-
var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
+
-
var parentEl=what.offsetParent;
+
-
while (parentEl!=null){
+
-
totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop;
+
-
parentEl=parentEl.offsetParent;
+
-
}
+
-
return totaloffset;
+
-
},
+
-
 
+
-
showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
+
-
if (this.ie || this.firefox)
+
-
this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
+
-
if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){
+
-
if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one
+
-
obj2.parentNode.className="selected"
+
-
obj.visibility="visible"
+
-
}
+
-
else if (e.type=="click")
+
-
obj.visibility="hidden"
+
-
},
+
-
 
+
-
iecompattest:function(){
+
-
return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
+
-
},
+
-
 
+
-
clearbrowseredge:function(obj, whichedge){
+
-
var edgeoffset=0
+
-
if (whichedge=="rightedge"){
+
-
var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15
+
-
this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
+
-
if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure)  //move menu to the left?
+
-
edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth
+
-
}
+
-
else{
+
-
var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset
+
-
var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18
+
-
this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
+
-
if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up?
+
-
edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight
+
-
if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either?
+
-
edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge
+
-
}
+
-
this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
+
-
}
+
-
return edgeoffset
+
-
},
+
-
 
+
-
dropit:function(obj, e, dropmenuID){
+
-
if (this.dropmenuobj!=null){ //hide previous menu
+
-
this.dropmenuobj.style.visibility="hidden" //hide menu
+
-
if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
+
-
if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
+
-
this.previousmenuitem.parentNode.className=""
+
-
}
+
-
}
+
-
this.clearhidemenu()
+
-
if (this.ie||this.firefox){
+
-
obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
+
-
obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
+
-
this.dropmenuobj=document.getElementById(dropmenuID)
+
-
this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
+
-
this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
+
-
this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
+
-
this.showhide(this.dropmenuobj.style, e, obj)
+
-
this.dropmenuobj.x=this.getposOffset(obj, "left")
+
-
this.dropmenuobj.y=this.getposOffset(obj, "top")
+
-
this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
+
-
this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
+
-
this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
+
-
this.positionshim() //call iframe shim function
+
-
}
+
-
},
+
-
 
+
-
contains_firefox:function(a, b) {
+
-
while (b.parentNode)
+
-
if ((b = b.parentNode) == a)
+
-
return true;
+
-
return false;
+
-
},
+
-
 
+
-
dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
+
-
var evtobj=window.event? window.event : e
+
-
if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
+
-
this.delayhidemenu(obj2)
+
-
else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
+
-
this.delayhidemenu(obj2)
+
-
},
+
-
 
+
-
delayhidemenu:function(obj2){
+
-
this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
+
-
},
+
-
 
+
-
clearhidemenu:function(){
+
-
if (this.delayhide!="undefined")
+
-
clearTimeout(this.delayhide)
+
-
},
+
-
 
+
-
positionshim:function(){ //display iframe shim function
+
-
if (this.enableiframeshim && typeof this.shimobject!="undefined"){
+
-
if (this.dropmenuobj.style.visibility=="visible"){
+
-
this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
+
-
this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
+
-
this.shimobject.style.left=this.dropmenuobj.style.left
+
-
this.shimobject.style.top=this.dropmenuobj.style.top
+
-
}
+
-
this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
+
-
}
+
-
},
+
-
 
+
-
hideshim:function(){
+
-
if (this.enableiframeshim && typeof this.shimobject!="undefined")
+
-
this.shimobject.style.display='none'
+
-
},
+
-
 
+
-
isSelected:function(menuurl){
+
-
var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
+
-
return (tabdropdown.currentpageurl==menuurl)
+
-
},
+
-
 
+
-
init:function(menuid, dselected){
+
-
this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
+
-
var menuitems=document.getElementById(menuid).getElementsByTagName("a")
+
-
for (var i=0; i<menuitems.length; i++){
+
-
if (menuitems[i].getAttribute("rel")){
+
-
var relvalue=menuitems[i].getAttribute("rel")
+
-
document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
+
-
menuitems[i].onmouseover=function(e){
+
-
var event=typeof e!="undefined"? e : window.event
+
-
tabdropdown.dropit(this, event, this.getAttribute("rel"))
+
-
}
+
-
}
+
-
if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
+
-
menuitems[i].parentNode.className+=" selected default"
+
-
var setalready=true
+
-
}
+
-
else if (parseInt(dselected)==i)
+
-
menuitems[i].parentNode.className+=" selected default"
+
-
}
+
-
}
+
-
 
+
-
}
+
-
</script>
+
-
<style>
+
-
.ddcolortabs{
+
-
padding: 0;
+
-
width: 100%;
+
-
background: transparent;
+
-
voice-family: "\"}\"";
+
-
voice-family: inherit;
+
-
}
+
-
 
+
-
.ddcolortabs ul{
+
-
font: normal 11px Arial, Verdana, sans-serif;
+
-
margin:0;
+
-
padding:0;
+
-
list-style:none;
+
-
}
+
-
 
+
-
.ddcolortabs li{
+
-
display:inline;
+
-
margin:0 2px 0 0;
+
-
padding:0;
+
-
text-transform:uppercase;
+
-
}
+
-
 
+
-
 
+
-
.ddcolortabs a{
+
-
float:left;
+
-
color: white;
+
-
background: black url(media/color_tabs_left.gif) no-repeat left top;
+
-
margin:0 2px 0 0;
+
-
padding:0 0 1px 3px;
+
-
text-decoration:none;
+
-
letter-spacing: 1px;
+
-
}
+
-
 
+
-
.ddcolortabs a span{
+
-
float:left;
+
-
display:block;
+
-
background: transparent url(media/color_tabs_right.gif) no-repeat right top;
+
-
padding: 4px 8px 2px 7px;
+
-
}
+
-
 
+
-
.ddcolortabs a span{
+
-
float:none;
+
-
}
+
-
 
+
-
.ddcolortabs a:hover{
+
-
background-color: #591f20;
+
-
}
+
-
 
+
-
.ddcolortabs a:hover span{
+
-
background-color: #591f20;
+
-
}
+
-
 
+
-
.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
+
-
background-color: #591f20;
+
-
}
+
-
 
+
-
.ddcolortabsline{
+
-
clear: both;
+
-
padding: 0;
+
-
width: 100%;
+
-
height: 8px;
+
-
line-height: 8px;
+
-
background: black;
+
-
border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
+
-
}
+
-
 
+
-
/* ######### Style for Drop Down Menu ######### */
+
-
 
+
-
.dropmenudiv_a{
+
-
position:absolute;
+
-
top: 0;
+
-
border: 1px solid black; /*THEME CHANGE HERE*/
+
-
border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
+
-
border-bottom-width: 0;
+
-
font:normal 12px Arial;
+
-
line-height:18px;
+
-
z-index:100;
+
-
background-color: white;
+
-
width: 200px;
+
-
visibility: hidden;
+
-
}
+
-
 
+
-
 
+
-
.dropmenudiv_a a{
+
-
width: auto;
+
-
display: block;
+
-
text-indent: 5px;
+
-
border-top: 0 solid #678b3f;
+
-
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
+
-
padding: 2px 0;
+
-
text-decoration: none;
+
-
color: black;
+
-
}
+
-
 
+
-
* html .dropmenudiv_a a{ /*IE only hack*/
+
-
width: 100%;
+
-
}
+
-
 
+
-
.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
+
-
background-color: #8a3c3d;
+
-
color: white;
+
-
}
+
-
</style>
+
-
</head>
+
-
 
+
-
<!-- CSS for Drop Down Tabs Menu #1 -->
+
-
<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
+
-
 
+
-
<div id="colortab" class="ddcolortabs">
+
-
<ul>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
+
-
<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
+
-
</ul>
+
-
</div>
+
-
<div class="ddcolortabsline">&nbsp;</div>
+
-
<div id="dropmenu1_a" class="dropmenudiv_a">
+
-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
+
-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
+
-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
+
-
</div>
+
-
<script type="text/javascript">
+
-
//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
+
-
tabdropdown.init("colortab", 3)
+
-
</script>
+
-
</html>
+
-
For more information, please see [https://www.wiki.ed.ac.uk/display/iGEM2008/HOME our internal wiki].
+
== Calendar ==
-
== Calender ==
+
Click on a date to see a summary of labwork done on that day. Up-to-date to end 12 October. Best viewed with Mozilla Firefox.
<table>
<table>
Line 323: Line 35:
{| align="center"
{| align="center"
|-valign="top"
|-valign="top"
-
|align="center" width="200pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
+
|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
-
|align="center" width="200pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
+
|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
-
|align="center" width="200pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
+
|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
 +
|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=09}}
 +
|align="center" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=10}}
|}
|}
-
== Summary of samples: ==
+
== Summary of samples ==
-
 
+
-
[[PCR products]]
+
-
[[Ligation products]]
+
-
[[Minipreps]]
+
-
[[Maxipreps]]
+
-
[[Plates]]
+
-
[[Gels]]
+
-
 
+
-
'''P1:''' ''dxs'' from ''Eschaerichia coli'' JM109 (or K12?)<br />
+
-
'''P2:''' ''appY'' from ''E. coli'' JM109 (or K12?)<br />
+
-
'''P3:''' ''glgC'' from ''E. coli'' JM109 (or K12?)<br />
+
-
'''P4:''' fusion PCR for BABEL1+''glgC''<br />
+
-
'''P5:''' fusion PCR for BABEL2+''glgC''<br />
+
-
'''P6:''' mutagenic PCR to remove EcoRI site 1 from ''glgC''<br />
+
-
'''P7:''' mutagenic PCR to remove EcoRI site 2 from ''glgC''<br />
+
-
'''P8:''' ''crtB'' (from ''Pantoea ananatis'' cells)<br />
+
-
'''P9:''' ''crtI'' (from Douglas's maxiprep of the mutated ''crtIB'' plasmid)<br />
+
-
'''P10:''' ''crtY'' (from ''P. ananatis'' cells). Failed.<br />
+
-
'''P11:''' fusion PCR for rbs+''dxs''. Failed.<br />
+
-
'''P12:''' repeat fusion PCR for rbs+''dxs'', using L11 and primers rbs2fclon1+pSB1A2insr1.<br />
+
-
'''P13 and P14:''' MABEL mutation of site 1 on ''glgC'' mutant clones M19 and M22.<br />
+
-
'''P15:''' fusion PCR for rbs+''appY''<br />
+
-
'''P16, P17, P18 and P19:''' first attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
+
-
'''P20, P21, P22 and P23:''' second attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
+
-
'''P24, P25:''' repeat PCR for ''cenA'' and ''cex'' using Pfu. Failed.<br />
+
-
'''P26:''' Positive control PCR with primers fd1 and rd1 to amplify 16S rRNA gene ''rrnB'' from ''Cellulomonas fimi''.<br />
+
-
'''P27:''' ''crtY'' from ''P. ananatis'' cells. - Failed<br />
+
-
'''P28, P29:''' ''crtB'' and ''crtI'' from L18 and L19.<br />
+
-
'''P30:''' Repeat positive control ''rrnB'' from ''C. fimi'', annealing 1 minute.<br />
+
-
'''P31:''' pZntA promoter<br />
+
-
'''P32-P35:''' repeat PCR for ''cenA'', ''cenB'', ''cenC'' and ''cex'', annealing 1 minute. Failed.<br />
+
-
'''P36:''' M43 (''glgC''-mut1,2) with primers glgCf2/glgCr2 (25.07.08: AM)<br />
+
-
'''P37:''' ''crtY'' from ''P. ananatis''. (25.07.08: AM) - Failed<br />
+
-
'''P38~P41:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from heat-killed cell suspension.<br />
+
-
'''P42~P45:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from 'impure' DNA solution.<br />
+
-
'''P46~P47:''' ''cenA'' and ''cex'' from 'impure' DNA solution, annealing 65C. Failed.<br />
+
-
'''P48:''' ''crtY'' (new primer mixture) (previously P38a) (30.07.08: CF) - Failed<br />
+
-
'''P49:''' Recreation of P12 (rbs+''dxs'') (previously P39a) (30.07.08: CF)<br />
+
-
'''P50:''' third mutagenesis of ''glgc''-mut1,2 (04.08.08: YAN)<br />
+
-
'''P51:''' pCstA from ''E. coli'' cells (06.08.08: CF) - Failed<br />
+
-
'''P52:''' ''cenA'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
+
-
'''P53:''' ''cex'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
+
-
'''P54:''' pCstA from ''E. coli'' cells - Purified (07.08.08: CF)<br />
+
-
 
+
-
==== Ligations ====
+
-
 
+
-
'''L1:''' Edinbrick1+''dxs''<br />
+
-
'''L2:''' Edinbrick1+''appY''<br />
+
-
'''L3:''' Babel1+''glgC''<br />
+
-
'''L4:''' Babel2+''glgC''<br />
+
-
'''L5:''' Edinbrick1+''appY'' repeat (initially labelled L4 in error)<br />
+
-
'''L6:''' Self ligation of mutagenic PCR P6<br />
+
-
'''L7:''' Self ligation of mutagenic PCR P7<br />
+
-
'''L8:''' Ligation of synthetic ribosome binding site to M2 (BBa_K118000)<br />
+
-
'''L9:''' Edinbrick1+''crtB''<br />
+
-
'''L10:''' Edinbrick1+''crtI'' (mutant lacking PstI sites, I hope)<br />
+
-
'''L11:''' repeat ligation of synthetic ribosome binding site to M2 (BBa_K118000)<br />
+
-
'''L12:''' rbs ligated with ''appY''.<br />
+
-
'''L13 and L14:''' self ligations of ''glgC'' mutation reactions P13 and P14.<br />
+
-
'''L15:''' ligation of Edinbrick1 with rbs+''dxs'' fusion PCR product (P12). - Failed<br />
+
-
'''L16:''' Ligation of Edinbrick1 with rbs+''appY'' fusion PCR product (P15) (17/07/08) - Failed<br />
+
-
'''L17:''' Ligation of ''crtB'' (M36) to rbs (22/07/08)<br />
+
-
'''L18:''' Ligation of ''crtI'' (M42) to rbs (22/07/08)<br />
+
-
'''L19:''' pSB1A2+rbs+''dxs'' ligation product (From P12) (25/07/08: Yan/OG)<br />
+
-
'''L20:''' pSB1A2+rbs+''appY'' ligation product (From P15) (25/07/08: Yan/OG)<br />
+
-
'''L21:''' pSB1A2+rbs+''crtB'' ligation product (From P28) (25/07/08: Yan/OG)<br />
+
-
'''L22:''' pSB1A2+rbs+''crtI'' ligation product (From P29) (25/07/08: Yan/OG)<br />
+
-
'''L23:''' PzntA+pSB1A2 ligation product (From P31) (25/07/08: Yan/OG)<br />
+
-
'''L24:''' Ligation of ''crtE'' to pSB1A2 (25/07/08: CF)<br />
+
-
'''L25:''' Ligation of ''glgC'' double mutant (P36) to Edinbrick 1 (28.07.2008: AM)<br />
+
-
'''L26:''' Ligation of Edi+P15 XP - rbs+''appY'' (30.07.2008: HX)<br />
+
-
'''L27:''' Ligation of Edi+P28 XP - rbs+''crtB'' (30.07.2008: HX)<br />
+
-
'''L28:''' Putative ''cenA'' ligated to Edinbrick1 (30.07.08: AM)<br />
+
-
'''L29:''' Putative ''cex'' ligated to Edinbrick1 (30.07.08: AM)<br />
+
-
'''L30:''' Self-ligation of P15 (3rd mutagenesis of ''glgC''-mut1,2) (05.08.08: HX)<br />
+
-
'''L31:''' Ligation of P36 (''glgC''-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)<br />
+
-
'''L32:''' Ligation of rbs+''crtB'' (M67) to rbs+''crtI'' (M50) to create ''crtB''/''crtI'' (07.08.08: AM, Yan)<br />
+
-
'''L33:''' Ligation of PcstA (P54) to Edinbrick1 (08.08.08: OG)<br />
+
-
 
+
-
==== Minipreps ====
+
-
 
+
-
'''M1 to M6:''' pSB1A2+''dxs'' transformants. *M2, M3, M4 and M6* all looked correct on a gel. *M2* was sequenced to confirm identity and was maxiprepped as *X2*.<br />
+
-
'''M7:''' Babel1+''glgC'' transformant. Did not look right on gel.<br />
+
-
'''M8 to M12:''' Babel2+''glgC''_ transformants. *M10 and M11* both looked good on a gel, but sequencing showed that both had the insert in the reverse orientation.<br />
+
-
'''M13 to M18:''' pSB1A2+''appY'' transformants. Gel 10 showed that M15 to M18, and possibly M14, all seemed to have inserts the right size.<br />
+
-
'''M19 to M24:''' Babel2+''glgC'' clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site.<br />
+
-
'''M25 to M30:''' possible rbs+''dxs'' transformants.<br />
+
-
'''M31 to M36:''' ''crtB'' clones.<br />
+
-
'''M37 to M42:''' ''crtI'' clones.<br />
+
-
'''M43 to M47:''' possible ''glgC'' double mutants (both EcoRI sites gone)<br />
+
-
'''M48:''' a possible rbs+''dxs'' clone.<br />
+
-
'''M49 to M54:''' pSB1A2-rbs+''crtI''<br />
+
-
'''M55 to M60:''' PZntA promoter<br />
+
-
'''M61 to M66:''' BABEL2+rbs+''crtE''<br />
+
-
'''M67:''' rbs+''crtB''<br />
+
-
'''M68 to M71:''' pSB1A2+''glgC''-mut1,2 (31.07.08: AM, YAN)<br />
+
-
'''M72 to M75:''' pSB1A2+rbs+''dxs'' (31.07.08: AM, YAN)<br />
+
-
'''M76 to M79:''' BABEL2+''crtE'' (31.07.08: AM, YAN)<br />
+
-
'''M80 to M83:''' pSB1A2+rbs+''appY'' (04/08/08: YAN and OG)<br />
+
-
'''M84 to M85:''' pSB1A2+rbs+''crtB'' (04/08/08: YAN and OG)<br />
+
-
'''M86 to M90:''' pSB1A2+''cenA'' (04/08/08: YAN and OG)<br />
+
-
'''M91 to M96:''' pSB1A2+''cex''(04/08/08: YAN and OG)<br />
+
-
'''M97 to M104:''' pSB1A2+''cenA'' (05.08.08: YAN)<br />
+
-
'''M105 to M108:''' pSB1A2+''cex'' (05.08.08: YAN)<br />
+
-
==== Maxipreps ====
+
Up-to-date to end 28 October.
-
'''X1:''' BioBrick J33201<br />
+
[[Edinburgh/Notebook/PCR products|PCR products]]<br />
-
'''X2:''' pSB1A2+''dxs'' clone (as M2; BBa_K118000)<br />
+
[[Edinburgh/Notebook/Ligation products|Ligation products]]<br />
-
'''X3:''' pSB1A2+''appY'' (from Plate 21, streak 3) (18 July 2008)<br />
+
[[Edinburgh/Notebook/Minipreps|Minipreps]]<br />
-
'''X4:''' pSB1A2+''crtB'' (as M36; BBa_K118002) (25.07.08: A
+
[[Edinburgh/Notebook/Maxipreps|Maxipreps]]<br />
 +
[[Edinburgh/Notebook/Plates|Plates]]<br />

Latest revision as of 00:26, 30 October 2008

Calendar

Click on a date to see a summary of labwork done on that day. Up-to-date to end 12 October. Best viewed with Mozilla Firefox.

June
MTWTFSS
            [http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16] [http://2008.igem.org/Edinburgh/17_June_2008 17] [http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18] [http://2008.igem.org/Edinburgh/19_June_2008 19] [http://2008.igem.org/Edinburgh/20_June_2008 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23] [http://2008.igem.org/Edinburgh/24_June_2008 24] [http://2008.igem.org/Edinburgh/25_June_2008 25] [http://2008.igem.org/Edinburgh/26_June_2008 26] [http://2008.igem.org/Edinburgh/27_June_2008 27] [http://2008.igem.org/Edinburgh/28_June_2008 28] [http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Edinburgh/1_July_2008 1] [http://2008.igem.org/Edinburgh/2_July_2008 2] [http://2008.igem.org/Edinburgh/3_July_2008 3] [http://2008.igem.org/Edinburgh/4_July_2008 4] [http://2008.igem.org/Edinburgh/5_July_2008 5] [http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7] [http://2008.igem.org/Edinburgh/8_July_2008 8] [http://2008.igem.org/Edinburgh/9_July_2008 9] [http://2008.igem.org/Edinburgh/10_July_2008 10] [http://2008.igem.org/Edinburgh/11_July_2008 11] [http://2008.igem.org/Edinburgh/12_July_2008 12] [http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14] [http://2008.igem.org/Edinburgh/15_July_2008 15] [http://2008.igem.org/Edinburgh/16_July_2008 16] [http://2008.igem.org/Edinburgh/17_July_2008 17] [http://2008.igem.org/Edinburgh/18_July_2008 18] [http://2008.igem.org/Edinburgh/19_July_2008 19] [http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21] [http://2008.igem.org/Edinburgh/22_July_2008 22] [http://2008.igem.org/Edinburgh/23_July_2008 23] [http://2008.igem.org/Edinburgh/24_July_2008 24] [http://2008.igem.org/Edinburgh/25_July_2008 25] [http://2008.igem.org/Edinburgh/26_July_2008 26] [http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28] [http://2008.igem.org/Edinburgh/29_July_2008 29] [http://2008.igem.org/Edinburgh/30_July_2008 30] [http://2008.igem.org/Edinburgh/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Edinburgh/1_August_2008 1] [http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2] [http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4] [http://2008.igem.org/Edinburgh/5_August_2008 5] [http://2008.igem.org/Edinburgh/6_August_2008 6] [http://2008.igem.org/Edinburgh/7_August_2008 7] [http://2008.igem.org/Edinburgh/8_August_2008 8] [http://2008.igem.org/Edinburgh/9_August_2008 9] [http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11] [http://2008.igem.org/Edinburgh/12_August_2008 12] [http://2008.igem.org/Edinburgh/13_August_2008 13] [http://2008.igem.org/Edinburgh/14_August_2008 14] [http://2008.igem.org/Edinburgh/15_August_2008 15] [http://2008.igem.org/Edinburgh/16_August_2008 16] [http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18] [http://2008.igem.org/Edinburgh/19_August_2008 19] [http://2008.igem.org/Edinburgh/20_August_2008 20] [http://2008.igem.org/Edinburgh/21_August_2008 21] [http://2008.igem.org/Edinburgh/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25] [http://2008.igem.org/Edinburgh/26_August_2008 26] [http://2008.igem.org/Edinburgh/27_August_2008 27] [http://2008.igem.org/Edinburgh/28_August_2008 28] [http://2008.igem.org/Edinburgh/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]
September
MTWTFSS
[http://2008.igem.org/Edinburgh/1_September_2008 1] [http://2008.igem.org/Edinburgh/2_September_2008 2] [http://2008.igem.org/Edinburgh/3_September_2008 3] [http://2008.igem.org/wiki/index.php?title=Edinburgh/4_September_2008&action=edit 4] [http://2008.igem.org/Edinburgh/5_September_2008 5] [http://2008.igem.org/wiki/index.php?title=Edinburgh/6_September_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Edinburgh/7_September_2008&action=edit 7]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/8_September_2008&action=edit 8] [http://2008.igem.org/Edinburgh/9_September_2008 9] [http://2008.igem.org/Edinburgh/10_September_2008 10] [http://2008.igem.org/Edinburgh/11_September_2008 11] [http://2008.igem.org/Edinburgh/12_September_2008 12] [http://2008.igem.org/wiki/index.php?title=Edinburgh/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_September_2008&action=edit 14]
[http://2008.igem.org/Edinburgh/15_September_2008 15] [http://2008.igem.org/Edinburgh/16_September_2008 16] [http://2008.igem.org/Edinburgh/17_September_2008 17] [http://2008.igem.org/Edinburgh/18_September_2008 18] [http://2008.igem.org/Edinburgh/19_September_2008 19] [http://2008.igem.org/wiki/index.php?title=Edinburgh/20_September_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_September_2008&action=edit 21]
[http://2008.igem.org/Edinburgh/22_September_2008 22] [http://2008.igem.org/Edinburgh/23_September_2008 23] [http://2008.igem.org/Edinburgh/24_September_2008 24] [http://2008.igem.org/Edinburgh/25_September_2008 25] [http://2008.igem.org/Edinburgh/26_September_2008 26] [http://2008.igem.org/Edinburgh/27_September_2008 27] [http://2008.igem.org/Edinburgh/28_September_2008 28]
[http://2008.igem.org/Edinburgh/29_September_2008 29] [http://2008.igem.org/Edinburgh/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Edinburgh/1_October_2008 1] [http://2008.igem.org/Edinburgh/2_October_2008 2] [http://2008.igem.org/Edinburgh/3_October_2008 3] [http://2008.igem.org/Edinburgh/4_October_2008 4] [http://2008.igem.org/Edinburgh/5_October_2008 5]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/6_October_2008&action=edit 6] [http://2008.igem.org/Edinburgh/7_October_2008 7] [http://2008.igem.org/Edinburgh/8_October_2008 8] [http://2008.igem.org/Edinburgh/9_October_2008 9] [http://2008.igem.org/Edinburgh/10_October_2008 10] [http://2008.igem.org/Edinburgh/11_October_2008 11] [http://2008.igem.org/Edinburgh/12_October_2008 12]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/13_October_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_October_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Edinburgh/15_October_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Edinburgh/16_October_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Edinburgh/17_October_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Edinburgh/18_October_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Edinburgh/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/20_October_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_October_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Edinburgh/22_October_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Edinburgh/23_October_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Edinburgh/24_October_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Edinburgh/25_October_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Edinburgh/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/27_October_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Edinburgh/28_October_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Edinburgh/29_October_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Edinburgh/30_October_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Edinburgh/31_October_2008&action=edit 31]

Summary of samples

Up-to-date to end 28 October.

PCR products
Ligation products
Minipreps
Maxipreps
Plates

Retrieved from "http://2008.igem.org/Team:Edinburgh/Notebook"