Edinburgh/18 August 2008

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Latest revision as of 21:39, 29 October 2008

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Week 10

Monday 18 August 08

Minipreps:
- M144-M147: Minipreps of cenA (1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.
- M148-M151: Minipreps of cex (1,2,3,4).
- M152-M155: Minipreps of dxs-crtE (1,2,3,4).(OG)
Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)
Maxipreps:
- X11: Zea mays isoamylase 1 (SU1+Pzntr22).
- X12: Maxiprep of Zea mays isoamylase 2 (ISO2+Pzntr22). (HX)
Combined biobricks:
- L42: dxs+LIMS (M137) to appY (M82).
- L43: crtB+crtI (M109) to appY (M82).
- L44: PcstA (M130) to lacZ. (YAN)
Blunt-ended ligations L45~L47 of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)

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@@ Line 1: / Line 1: @@
-=== Week 10 ===
+<div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div>
-==== Monday 18 August 08 ====
-*M144-M147: Minipreps of cenA(1,2,3,4). M148-M151: Minipreps of cex(1,2,3,4). M152-M155: Munipreps of dxs-crtE (1,2,3,4). Another mistake made, I used methanol(920ul) instead of ethanol for M144-M147.(OG)
+'''[[Edinburgh/17_August_2008|< Previous Entry]] | [[Team:Edinburgh/Notebook|Notebook Index]] | [[Edinburgh/19_August_2008|Next Entry >]]'''
-*Minipreps 144-155 were digested with EcoRI/PstI. This need running on a gel tomorrow. (AH)
-*X11: Maxiprep of Zea mays isoamylase 1 (isa1+pZntr22). X12: Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX)
+== Week 10 ==
-*Combined biobricks:
+=== Monday 18 August 08 ===
-L42: dxs+lims (M137) to appY (M82).
-L43: crtB+crtI (M109) to appY (M82).
+* Minipreps:
-L44: pstA (M130) to lacB. (YAN)
+**'''M144-M147:''' Minipreps of ''cenA'' (1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.<br>
-*Blunt-ended ligations L45~L47 of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
+**'''M148-M151:''' Minipreps of ''cex'' (1,2,3,4).<br>
-*Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
+**'''M152-M155:''' Minipreps of ''dxs-crtE'' (1,2,3,4).(OG)
+* Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)
+* Maxipreps:
+**'''X11:''' Zea mays isoamylase 1 (''SU1''+P''zntr22'').<br>
+**'''X12:''' Maxiprep of Zea mays isoamylase 2 (''ISO2''+P''zntr22''). (HX)
+* Combined biobricks:
+**'''L42:''' ''dxs''+''LIMS'' (M137) to ''appY'' (M82).<br>
+**'''L43:''' ''crtB''+''crtI'' (M109) to ''appY'' (M82).<br>
+**'''L44:''' P''cstA'' (M130) to ''lacZ''. (YAN)
+* Blunt-ended ligations '''L45~L47''' of purified P71A (''glgC'' mut 1+2), P71 and P72 (both ''glgC'' mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
+* Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
+'''[[Edinburgh/19_August_2008|Next Entry >]]'''