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User:University of Washington/22 August 2008
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==LuxR from pLac== | ==LuxR from pLac== | ||
-Ligation prodouct of I0462 and R0010 was transformed into DH5a+LacIq cells, and plated on amp. | -Ligation prodouct of I0462 and R0010 was transformed into DH5a+LacIq cells, and plated on amp. | ||
| + | |||
| + | ==LuxR from AraC and TetR(Faifan)== | ||
| + | |||
| + | -Got few colonies for LuxR construction, 1 for GFP | ||
| + | |||
| + | -Re-inoculated the culture from transformation left over that was stored in the fridge from yesterday(Thurs, 8/21/8). | ||
| + | *concentrate the culture by spinning down, taking 700 ul liquid out(150 ul left), re-suspending the palates and inoculating that on Kan plates. | ||
| + | *Let them grow at room temp. | ||
| + | |||
| + | ==MG1655Z1(Faifan)== | ||
| + | |||
| + | -minipreped and ran gel | ||
| + | *5 ul DNA: Appeared the band still! | ||
| + | *25 ul DNA: Didn't stay down the well. =[ | ||
| + | |||
| + | -Re-streaked the culture selected from culture#3 Tsy plate on Amp, Kan, Cam, and Tet plates. Let them grow at room temp. | ||
| + | |||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 21:51, 22 August 2008
LuxR from pLac
-Ligation prodouct of I0462 and R0010 was transformed into DH5a+LacIq cells, and plated on amp.
LuxR from AraC and TetR(Faifan)
-Got few colonies for LuxR construction, 1 for GFP
-Re-inoculated the culture from transformation left over that was stored in the fridge from yesterday(Thurs, 8/21/8).
- concentrate the culture by spinning down, taking 700 ul liquid out(150 ul left), re-suspending the palates and inoculating that on Kan plates.
- Let them grow at room temp.
MG1655Z1(Faifan)
-minipreped and ran gel
- 5 ul DNA: Appeared the band still!
- 25 ul DNA: Didn't stay down the well. =[
-Re-streaked the culture selected from culture#3 Tsy plate on Amp, Kan, Cam, and Tet plates. Let them grow at room temp.
Back to Team:University_of_Washington/Notebook#Notebook
