Team:Hawaii/Notebook/2008-08-22

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== Wetlab work ==
== Wetlab work ==
===Verification of transformants===
===Verification of transformants===
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[[Image:082208colonypcr.jpg|right|thumb|300px|EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Five microliters of PCR reaction were loaded into each lane.]]
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[[Image:082208colonypcr.jpg|right|thumb|400px|EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Five microliters of PCR reaction were loaded into each lane.]]
:<strong> Grace</strong>
:<strong> Grace</strong>
{|class=wikitable border=1 align=center
{|class=wikitable border=1 align=center
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:* Colony PCR of transformants
:* Colony PCR of transformants
:* Ran PCR reaction on 2% agarose gel
:* Ran PCR reaction on 2% agarose gel
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::* Can't tell 15bp difference between GFP and rbs+GFP
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:::* Restreaked single colony and inoculated for plasmid prep to confirm
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::* nir+rbs colonies #11-19 appear promising (larger than nir)
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:::* Restreaked colony #19 and inoculated for plasmid prep to confirm
===Plasmid prep of J33207===
===Plasmid prep of J33207===
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:<strong>Grace</strong>
:<strong>Grace</strong>
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== Drylab Work ==
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===Ligation===
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<strong>Margaret</strong>
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===Name of Task===
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:*ligation of rep+pSB1A3, P1lytic region+pSB1A3, aada(BB&pRL1383a)+pSB1A3
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:<strong> name of person/people who performed the task</strong>
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
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:* e.g. read through papers, worked on proposal, etc.
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===Omega interposon===
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:<strong>Margaret</strong>
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:*streak on Sm&Sp plate to confirm insertion.
= Discussion =
= Discussion =

Latest revision as of 13:41, 23 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of transformants

EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Five microliters of PCR reaction were loaded into each lane.
Grace
Construct Colony forming units
nir+rbs 187
plac+rbs 0
rbs+GFP 1
GFP+tt 0
  • Colony PCR of transformants
  • Ran PCR reaction on 2% agarose gel
  • Can't tell 15bp difference between GFP and rbs+GFP
  • Restreaked single colony and inoculated for plasmid prep to confirm
  • nir+rbs colonies #11-19 appear promising (larger than nir)
  • Restreaked colony #19 and inoculated for plasmid prep to confirm

Plasmid prep of J33207

Grace

Overnight RE digest

Grace
  • BB-pRL1383a and J33207 with XbaI and PstI
  • pSB1A2 with EcoRI and PstI
  • GFP, GFPf, nir with EcoRI and SpeI

Made LB+amp100 plates

Grace

Ligation

Margaret

  • ligation of rep+pSB1A3, P1lytic region+pSB1A3, aada(BB&pRL1383a)+pSB1A3

Omega interposon

Margaret
  • streak on Sm&Sp plate to confirm insertion.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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