Team:Hawaii/Notebook/2008-08-24

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:* Ran PCR reactions on 3% agarose gel at __V for __ hours.
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:* PCR machine crashed. Will redo PCR tomorrow.
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===Restriction Digest===
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:<strong>Margaret</strong>
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:* pSB1A3 with EcoRI and PstI, pSB1A3 with SpeI & PstI, rep (gel purified PCR product from 8/11/ with XbaI & PstI, and P1 lytic region (gel purified PCR product from 8/11/08) with XbaI & PstI.
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===Ligation & Transformation into DH5 alpha cells===
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:<strong>Margaret</strong>
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:* I14032 (synthetic construct of lac promoter) + B0030 (synthetic construct of 0.6 RBS),  I14032 (synthetic construct of lac promoter) + B0034 (synthetic construct of 0.1 RBS)
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:* pSB1A3 + [I14032+B0030], pSB1A3 + [I14032+B0034], (-) control= pSB1A3 to itself, (+) control for transformation is pUC18.
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===Colony PCR Gel from [[Team:Hawaii/Notebook/2008-08-23|yesterday]]===
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:<strong>Margaret</strong>
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[[Image:colony_pcr_8_23_1.jpg|right|thumb|300px|Colony PCR to verify aada regions, P1, rep.]]
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[[Image:colony_pcr_8_23_2.jpg|right|thumb|300px|Colony PCR to verify aada regions, P1, rep.]]
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Latest revision as of 04:47, 25 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of transformants

Grace
Construct Colony forming units
plac+rbs 0
GFP+tt 0
GFPf+tt 0
(-) control 0

Verification of plasmid preps (cont.)

Grace
  • PCR machine crashed. Will redo PCR tomorrow.

Restriction Digest

Margaret
  • pSB1A3 with EcoRI and PstI, pSB1A3 with SpeI & PstI, rep (gel purified PCR product from 8/11/ with XbaI & PstI, and P1 lytic region (gel purified PCR product from 8/11/08) with XbaI & PstI.

Ligation & Transformation into DH5 alpha cells

Margaret
  • I14032 (synthetic construct of lac promoter) + B0030 (synthetic construct of 0.6 RBS), I14032 (synthetic construct of lac promoter) + B0034 (synthetic construct of 0.1 RBS)
  • pSB1A3 + [I14032+B0030], pSB1A3 + [I14032+B0034], (-) control= pSB1A3 to itself, (+) control for transformation is pUC18.

Colony PCR Gel from yesterday

Margaret
Colony PCR to verify aada regions, P1, rep.
File:Colony pcr 8 23 2.jpg
Colony PCR to verify aada regions, P1, rep.



Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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