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Team:Hawaii/Notebook/2008-08-24
From 2008.igem.org
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|align=center|GFP+tt | |align=center|GFP+tt | ||
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| + | |align=center|(-) control | ||
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:* PCR machine crashed. Will redo PCR tomorrow. | :* PCR machine crashed. Will redo PCR tomorrow. | ||
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| + | ===Restriction Digest=== | ||
| + | :<strong>Margaret</strong> | ||
| + | |||
| + | :* pSB1A3 with EcoRI and PstI, pSB1A3 with SpeI & PstI, rep (gel purified PCR product from 8/11/ with XbaI & PstI, and P1 lytic region (gel purified PCR product from 8/11/08) with XbaI & PstI. | ||
| + | |||
| + | ===Ligation & Transformation into DH5 alpha cells=== | ||
| + | :<strong>Margaret</strong> | ||
| + | |||
| + | :* I14032 (synthetic construct of lac promoter) + B0030 (synthetic construct of 0.6 RBS), I14032 (synthetic construct of lac promoter) + B0034 (synthetic construct of 0.1 RBS) | ||
| + | :* pSB1A3 + [I14032+B0030], pSB1A3 + [I14032+B0034], (-) control= pSB1A3 to itself, (+) control for transformation is pUC18. | ||
| + | |||
| + | ===Colony PCR Gel from [[Team:Hawaii/Notebook/2008-08-23|yesterday]]=== | ||
| + | |||
| + | :<strong>Margaret</strong> | ||
| + | [[Image:colony_pcr_8_23_1.jpg|right|thumb|300px|Colony PCR to verify aada regions, P1, rep.]] | ||
| + | [[Image:colony_pcr_8_23_2.jpg|right|thumb|300px|Colony PCR to verify aada regions, P1, rep.]] | ||
| + | |||
| + | |||
| + | |||
= Discussion = | = Discussion = | ||
Latest revision as of 04:47, 25 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| Construct | Colony forming units |
|---|---|
| plac+rbs | 0 |
| GFP+tt | 0 |
| GFPf+tt | 0 |
| (-) control | 0 |
Verification of plasmid preps (cont.)
- Grace
- PCR machine crashed. Will redo PCR tomorrow.
Restriction Digest
- Margaret
- pSB1A3 with EcoRI and PstI, pSB1A3 with SpeI & PstI, rep (gel purified PCR product from 8/11/ with XbaI & PstI, and P1 lytic region (gel purified PCR product from 8/11/08) with XbaI & PstI.
Ligation & Transformation into DH5 alpha cells
- Margaret
- I14032 (synthetic construct of lac promoter) + B0030 (synthetic construct of 0.6 RBS), I14032 (synthetic construct of lac promoter) + B0034 (synthetic construct of 0.1 RBS)
- pSB1A3 + [I14032+B0030], pSB1A3 + [I14032+B0034], (-) control= pSB1A3 to itself, (+) control for transformation is pUC18.
Colony PCR Gel from yesterday
- Margaret
File:Colony pcr 8 23 2.jpg
Colony PCR to verify aada regions, P1, rep.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
