Alberta NINT/2 June 2008

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lab work (JD): Carried out mini-preps and sequencing on 8 colonies from O/Ns
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[[Team:Alberta_NINT/Notebook | < Back to notebook]]
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lab work (SD): Resuspended Term0+ and Term0- and annealed.  Digested T/A 0, K102002 and K102004 with BamHI and NcoI.
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[[Alberta_NINT/31_May_2008 | < Previous entry]] | [[Alberta_NINT/3_June_2008 | Next entry >]]
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lab work (JD):  
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                  Carried out mini-preps and sequencing on 8 colonies from O/Ns K102001.1 to K102001.8
 +
 
 +
lab work (SD):  
 +
 
 +
                  Resuspended Term0+ and Term0- and annealed.   
 +
                  Digested T/A 0, K102002 and K102004 with BamHI and NcoI.
 +
 
 +
 
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lab work (WM):
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                  All sequences looked good but K102010.2 is best
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                  Ligate into a low copy kan-resistant plasmid (pSB4K5) to make K102011
 +
                  Setup ligations of K102010.2/EcoRI+PstI with pSB4K5/EcoRI+PstI

Latest revision as of 22:14, 11 October 2008

< Back to notebook

< Previous entry | Next entry >

lab work (JD): 

                 Carried out mini-preps and sequencing on 8 colonies from O/Ns K102001.1 to K102001.8

lab work (SD): 

                 Resuspended Term0+ and Term0- and annealed.  
                 Digested T/A 0, K102002 and K102004 with BamHI and NcoI.


lab work (WM): 

                 All sequences looked good but K102010.2 is best
                 Ligate into a low copy kan-resistant plasmid (pSB4K5) to make K102011
                 Setup ligations of K102010.2/EcoRI+PstI with pSB4K5/EcoRI+PstI
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