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Alberta NINT/2 June 2008
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| - | lab work (JD): Carried out mini-preps and sequencing on 8 colonies from O/Ns K102001.1 to K102001.8 | + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] |
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| - | lab work (SD): Resuspended Term0+ and Term0- and annealed. Digested T/A 0, K102002 and K102004 with BamHI and NcoI. | + | [[Alberta_NINT/31_May_2008 | < Previous entry]] | [[Alberta_NINT/3_June_2008 | Next entry >]] |
| + | |||
| + | lab work (JD): | ||
| + | |||
| + | Carried out mini-preps and sequencing on 8 colonies from O/Ns K102001.1 to K102001.8 | ||
| + | |||
| + | lab work (SD): | ||
| + | |||
| + | Resuspended Term0+ and Term0- and annealed. | ||
| + | Digested T/A 0, K102002 and K102004 with BamHI and NcoI. | ||
| + | |||
| + | |||
| + | lab work (WM): | ||
| + | All sequences looked good but K102010.2 is best | ||
| + | Ligate into a low copy kan-resistant plasmid (pSB4K5) to make K102011 | ||
| + | Setup ligations of K102010.2/EcoRI+PstI with pSB4K5/EcoRI+PstI | ||
Latest revision as of 22:14, 11 October 2008
< Previous entry | Next entry >
lab work (JD):
Carried out mini-preps and sequencing on 8 colonies from O/Ns K102001.1 to K102001.8
lab work (SD):
Resuspended Term0+ and Term0- and annealed.
Digested T/A 0, K102002 and K102004 with BamHI and NcoI.
lab work (WM):
All sequences looked good but K102010.2 is best
Ligate into a low copy kan-resistant plasmid (pSB4K5) to make K102011
Setup ligations of K102010.2/EcoRI+PstI with pSB4K5/EcoRI+PstI
