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Team:Missouri Miners/Team
From 2008.igem.org
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!align="center"|[[Team:Missouri_Miners/Notebook|Notebook]] | !align="center"|[[Team:Missouri_Miners/Notebook|Notebook]] | ||
!align="center"|[[Team:Missouri_Miners/Articles|Articles]] | !align="center"|[[Team:Missouri_Miners/Articles|Articles]] | ||
| - | !align="center"|[[Team:Missouri_Miners/Sponsers| | + | !align="center"|[[Team:Missouri_Miners/Sponsers|Sponsors]] |
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| - | + | [[Image:Missouri_SandT_spelled_out.jpg|center]] | |
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| - | |[[Image: | + | In ''Pichia pastoris'', alcohol oxidase (AOX) is the first enzyme in the methanol utilization pathway. This enzyme is encoded by the AOXI gene. If exposed to an environment containing both methanol and ethanol, ''P. pastoris'' preferentially metabolizes ethanol. The production of the AOX enzyme is subject to the concentration of ethanol. This diauxic metabolism may be utilized as an ethanol sensor. When the AOXI promoter is fused with a gene encoding a fluorescent protein, the activation of the AOXI promoter may be detected by direct observation of fluorescence. Our project is the development of a device containing the AOXI promoter fused with a fluorescent protein gene to create an inexpensive ethanol sensor for a variety of applications. The concentration of ethanol in the environment may be deduced from the time period between exposure of bacteria carrying the device to ethanol and methanol, until the detection of fluorescence. |
| + | |[[Image:MissouriS&TTeam.JPG|320 px|right|thumb|We're here to save the world.]] | ||
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<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
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'''Advisors:''' | '''Advisors:''' | ||
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*'''Patrick VerSteeg''': Chemical and Biological Engineering | *'''Patrick VerSteeg''': Chemical and Biological Engineering | ||
*'''Barbi Wheelden''': Chemical and Biological Engineering | *'''Barbi Wheelden''': Chemical and Biological Engineering | ||
| - | + | *'''Rachel Klapper''': Chemical and Biological Engineering | |
| - | + | *'''Daniel Schwent''': Biology Major and Bioinformatics Minor | |
| + | <!--RING RING RING RING RING RING RING, BANANA PHONE--> | ||
| + | *'''Gregory Schmoll''': Biology Major and Chemistry Minor | ||
| + | *'''Brian Pink''': Chemical and Biological Engineering | ||
| + | *'''Michelle Brosnahan''': Biology Major | ||
| + | *'''Shradha Samuel''': Chemical and Biological Engineering | ||
| + | *'''''Escherichia coli''''': Chemical and Biological Engineering | ||
| + | *'''''Pichia pastoris''''': Chemical and Biological Engineering | ||
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<gallery> | <gallery> | ||
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Image:Cac.jpg|Cory Cheatham | Image:Cac.jpg|Cory Cheatham | ||
| - | Image: | + | Image:Pat.jpg|Patrick VerSteeg |
| + | Image:Barb.jpg| Barbi Wheelden | ||
| + | Image:Rachel.jpg| Rachel Klapper | ||
| + | Image:Danana.JPG| Daniel Schwent | ||
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| + | Image:Greg.jpg| Gregory Schmoll | ||
| + | Image:Typhoid-fever.jpg| Brian Pink | ||
| + | Image:Salmonella 01.jpg| Michelle Brosnahan | ||
| + | Image:Ulcer.jpg| Shradha Samuel | ||
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| + | Image:Ecoli2.jpg| ''Escherichia coli'' | ||
| + | Image:Beerandbread.jpg| ''Pichia pastoris'' (OK, it's really ''Saccharomyces cerevisia'' but it IS a yeast!) | ||
</gallery> | </gallery> | ||
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== '''What we did''' == | == '''What we did''' == | ||
| - | + | Read about it on our [https://2008.igem.org/Team:Missouri_Miners/Project Project Page] | |
== '''Where we're from''' == | == '''Where we're from''' == | ||
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| + | Missouri S&T, Formerly the Missouri School of Mines and the University of Missouri - Rolla is located in South Central Missouri, half way between St. Louis and Springfield, MO. | ||
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| + | [[Image:Rolla-logo.jpg]][[Image:Logo_1245_w.jpg|125px]] | ||
Latest revision as of 03:34, 30 October 2008
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In Pichia pastoris, alcohol oxidase (AOX) is the first enzyme in the methanol utilization pathway. This enzyme is encoded by the AOXI gene. If exposed to an environment containing both methanol and ethanol, P. pastoris preferentially metabolizes ethanol. The production of the AOX enzyme is subject to the concentration of ethanol. This diauxic metabolism may be utilized as an ethanol sensor. When the AOXI promoter is fused with a gene encoding a fluorescent protein, the activation of the AOXI promoter may be detected by direct observation of fluorescence. Our project is the development of a device containing the AOXI promoter fused with a fluorescent protein gene to create an inexpensive ethanol sensor for a variety of applications. The concentration of ethanol in the environment may be deduced from the time period between exposure of bacteria carrying the device to ethanol and methanol, until the detection of fluorescence. |  |
Who we are
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Advisors:
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What we did
Read about it on our Project Page
Where we're from
Missouri S&T, Formerly the Missouri School of Mines and the University of Missouri - Rolla is located in South Central Missouri, half way between St. Louis and Springfield, MO.
