Latest revision as of 03:05, 27 August 2008

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Things we did today

Margaret

Margaret

verified the colonies from the (pLac+B0030) ligation and transformation. no transformants for the B0034 ligation

EtBr stained 3% agarose gel ran at 60V for 3 hours. Two microliters of PCR reaction were loaded into each well.

Grace

Grace

Krystle

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 12: / Line 12: @@
 :*verified the colonies from the (pLac+B0030) ligation and transformation. no transformants for the B0034 ligation
-== Drylab Work ==
+===Verification of transformants (cont.)===
+[[Image:082508PCR.jpg|right|thumb|250px|EtBr stained 3% agarose gel ran at 60V for 3 hours. Two microliters of PCR reaction were loaded into each well.]]
+:<strong> Grace</strong>
-===Name of Task===
+:* PCR'd nir, nir+rbs, GFP, GFP+rbs
-:<strong> name of person/people who performed the task</strong>
+:* Ran on 3% agarose gel
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+===Restriction digest===
-:* e.g. read through papers, worked on proposal, etc.
+:<strong>Grace</strong>
+:* RE digested overnight:
+::* BB-pRL1383a and J33207 with XbaI and PstI
+::* C0012 with EcoRI and PstI (to isolate vector)
+::* nir with EcoRI and SpeI
+===Ligation===
+:<strong>Krystle</strong>
+:* Ligated overnight:
+::* plac + B0034 (both synthesized)
+::* plac + B0030 (both synthesized)
+::* gfpf (vector) + tt (synthesized)
+::* gfpf (re'd with E-S) + tt (synthesized)
 = Discussion =