Edinburgh/26 July 2008

From 2008.igem.org

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<title>Edinburgh iGEM 2008</title>
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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{| align="left"
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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|}
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:::: '''[[Edinburgh/25_July_2008|< Previous Entry]]'''
:::: '''[[Edinburgh/25_July_2008|< Previous Entry]]'''
Line 332: Line 6:
=== Saturday 26 July 08 ===
=== Saturday 26 July 08 ===
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* Transformed JM109 with 5μl of ligations labelled as L12, L15, L28, L29, L31 (note: these ligations seem to have been numbered based on the PCR product they were derived from rather than sequentially; they have been renumbered as '''L19 to L23'''), plus my own ligation ('''L24''') which was to transfer ''crtE'' from the BABEL vector (which is not an 'official' BioBrick vector, though it is fully compliant) to pSB1A2, so that we can submit it to the Registry. These were plated on '''plates 51 to 62.''' (CF)<br />
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* Transformed JM109 with 5μl of ligations L19 to L24 (pSB1A2+rbs+''dxs'', pSB1A2+rbs+''appY'', pSB1A2+rbs+''crtB'', pSB1A2+rbs+''crtI'', pSB1A2+''P<sub>zntA</sub>'', pSB1A2+''crtE''). These were plated on '''plates 51-62'''. (CF)<br />
<br />
<br />
:::: '''[[Edinburgh/27_July_2008|Next Entry >]]'''
:::: '''[[Edinburgh/27_July_2008|Next Entry >]]'''

Latest revision as of 20:36, 29 October 2008

< Previous Entry

Week 6

Saturday 26 July 08

  • Transformed JM109 with 5μl of ligations L19 to L24 (pSB1A2+rbs+dxs, pSB1A2+rbs+appY, pSB1A2+rbs+crtB, pSB1A2+rbs+crtI, pSB1A2+PzntA, pSB1A2+crtE). These were plated on plates 51-62. (CF)


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