Team:Chiba/Calendar-Home/20 August 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Input)
 
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-
'''OUTPUT TEAM'''
+
>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
-
*Transformation
+
-
Luciferase(J52008:2007,2008 I712019:2008),Lac Z(J33202:2007,2008),Venus(J32007:2007,2008),Craig Venus,T9002,F2620
+
-
結果:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
+
[[Team:Chiba/Calendar-Home/19 August 2008|19 August 2008 <]]|[[Team:Chiba/Calendar-Home/21 August 2008|> 21 August 2008]]
-
失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
+
 
 +
==Laboratory work==
 +
===Team:Input===
 +
'''[[Team:Chiba/protocol/PCR|PCR]]'''
 +
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
 +
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
 +
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
 +
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
 +
*[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)
 +
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
 +
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
 +
</tr>
 +
<tr>
 +
<td>DNA tamplate(&mu;L)</td>
 +
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>FW primer(&mu;L)</td>
 +
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
 +
</tr>
 +
<tr>
 +
<td>VR primer(&mu;L)</td>
 +
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
 +
</tr>
 +
<tr>
 +
<td>dNTPmix(&mu;L)</td>
 +
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 +
</tr>
 +
<tr>
 +
<td>thermo pol buffer(&mu;L)</td>
 +
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 +
</tr>
 +
<tr>
 +
<td>DNA pol(VENT)(&mu;L)</td>
 +
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O(&mu;L)</td>
 +
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL(&mu;L)</td>
 +
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
 +
</tr>
 +
</table>
 +
 
 +
-->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
             
 +
 +
<table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
 +
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
 +
</tr>
 +
<tr>
 +
<td>Sample DNA</td>
 +
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>loading dye(&mu;L)</td>
 +
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O(&mu;L)</td>
 +
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
 +
</tr>
 +
<tr>
 +
<td>Total(&mu;L)</td>
 +
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
 +
</tr>
 +
 
 +
</table>
 +
 
 +
-->1,2,3,4,5:none
 +
 
 +
'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 +
 
 +
*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
 +
*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
 +
*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
 +
*[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
 +
*[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
 +
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony
 +
 
 +
===Team:Communication===
 +
:(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 +
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
 +
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
 +
::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
 +
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
 +
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
 +
 
 +
 
 +
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0820-3.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>3</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>2</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>7</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>12μL</td>
 +
</tr>
 +
</table>
 +
 
 +
:From left;
 +
::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
 +
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
 +
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None
 +
|}
 +
 
 +
 
 +
 
 +
{|align="justify"
 +
|[[Image:Chiba-0820-4.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>10</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>4</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>10</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>24μL</td>
 +
</tr>
 +
</table>
 +
 
 +
:From left;
 +
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
 +
::*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
 +
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK
 +
|}
 +
 
 +
 
 +
 
 +
 
 +
{|align="justify"
 +
|[[Image:Chiba-0820.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>3</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>2</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>7</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>12μL</td>
 +
</tr>
 +
</table>
 +
 
 +
:From left;<br>
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
 +
|}
 +
 
 +
 
 +
--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
 +
 
 +
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No</td>
 +
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td>
 +
</tr>
 +
<tr>
 +
<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)</td>
 +
<td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
 +
</tr>
 +
<tr>
 +
<td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)</td>
 +
<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
 +
</tr>
 +
<tr>
 +
<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)</td>
 +
<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
 +
</tr>
 +
<tr>
 +
<td>[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)</td>
 +
<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
 +
</tr>
 +
<tr>
 +
<td>EcoRI</td>
 +
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 +
</tr>
 +
<tr>
 +
<td>PstI</td>
 +
<td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 +
</tr>
 +
<tr>
 +
<td>Buffer EcoRI</td>
 +
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>BSA</td>
 +
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td><td>10μL</td>
 +
</tr>
 +
</table>
 +
 
 +
 
 +
 
 +
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0820-2.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>3</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>2</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>7</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>12μL</td>
 +
</tr>
 +
</table>
 +
:From left;
 +
:(Single Digestion 1~4)
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
 +
:(Double Digestion 1~4)
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
 +
::*[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
 +
|}
 +
 
 +
===Team:Output===
 +
 
 +
[[Team:Chiba/protocol/transformation|Transformation]]
 +
*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
 +
*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
 +
*[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
 +
*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
 +
*YFP(P-GEX)-->colony(Amp)
 +
*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
 +
*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony

Latest revision as of 05:57, 30 October 2008

>Home | Notebook

19 August 2008 <|> 21 August 2008

Contents

Laboratory work

Team:Input

PCR

  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
  • [http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)
Sample No. 12345
DNA tamplate(μL) 11111
FW primer(μL) 55555
VR primer(μL) 55555
dNTPmix(μL) 1010101010
thermo pol buffer(μL) 1010101010
DNA pol(VENT)(μL) 11111
dH2O(μL) 6868686868
TOTAL(μL) 100100100100100

-->Gel Check


Sample No. 12345
Sample DNA 11111
loading dye(μL) 11111
dH2O(μL) 44444
Total(μL) 66666

-->1,2,3,4,5:none

Transformation

  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
  • [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
  • [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
  • [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony

Team:Communication

(18/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)


--->Gel Check
Chiba-0820-3.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None


Chiba-0820-4.JPG
Sample DNA 10
Loading Dye 4
dH2O 10
TOTAL 24μL
From left;
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK



Chiba-0820.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK


--->Digestion test

  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
Sample No 12345678
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) 3---3---
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -3---3--
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) --3---3-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) ---3---3
EcoRI 0.10.10.10.10.10.10.10.1
PstI ----0.10.10.10.1
Buffer EcoRI 11111111
BSA 11111111
dH2O 4.94.94.94.94.84.84.84.8
TOTAL 10μL10μL10μL10μL10μL10μL10μL10μL


--->Gel Check
Chiba-0820-2.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
(Single Digestion 1~4)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
(Double Digestion 1~4)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
  • [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
  • YFP(P-GEX)-->colony(Amp)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/20_August_2008"