UW Glycerol Stocks

From 2008.igem.org

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{| style="color:Gold;background-color:#0b0c0a;border-color:#6b0c6a;" cellpadding="4" cellspacing="5" border="5" width="99%" align="center"
{| style="color:Gold;background-color:#0b0c0a;border-color:#6b0c6a;" cellpadding="4" cellspacing="5" border="5" width="99%" align="center"
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington|Home]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington|<font color="gold">Home</font>]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Team|The Team]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Team|<font color="gold">The Team</font>]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Project|The Project]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Project|<font color="gold">The Project</font>]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Parts|Parts Submitted to the Registry]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Parts|<font color="gold">Parts Submitted to the Registry</font>]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Modeling|Modeling]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Modeling|<font color="gold">Modeling</font>]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Notebook|Notebook]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Notebook|<font color="gold">Notebook</font>]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Protocols|Protocols]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/Protocols|<font color="gold">Protocols</font>]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/BioBrick Parts Used| BioBrick Parts Used]]
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!align="center"; style="border-color:#6b0c6a"|[[Team:University_of_Washington/BioBrick Parts Used|<font color="gold">BioBrick Parts Used</font>]]
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!align="center"; style="border-color:#6b0c6a;background-color:#500050"|[[UW Glycerol Stocks]]
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!align="center"; style="border-color:#6b0c6a;background-color:#500050"|[[UW Glycerol Stocks|<font color="gold">Glycerol Stocks</font>]]
|}
|}
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<br>
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6/26/08<br\>
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{| style="text-align:center;color:Black;background-color:Gold;border-color:#6b0c6a;" border=2 cellpadding=1 cellspacing=1
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·C600+RP4<br\>
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!Date!!Part!!Strain!!Resistance
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·S17-1
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|-
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|valign=top rowspan=2|6/26/08
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6/27/08<br\>
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|align=left|RP4||C600||Amp
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·R0062 LuxR promoter<br\>
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|-
-
·R0080 AraC promoter<br\>
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|align=left|none||S17-1||
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·R0040 TetR promoter<br\>
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|-
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·BY4741 yeast culture
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|valign=top rowspan=4|6/27/08
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|align=left|R0062 LuxR promoter||||Amp
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7/1/08<br\>
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|-
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·I20260 Promoter J23101 Construct (in TOP10)<br\>
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|align=left|R0080 AraC promoter||MG1655||Amp
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·I20268 Promoter J23102 Construct (in TOP10)<br\>
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|-
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·I20269 Promoter J23150 Construct (in TOP10)<br\>
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|align=left|R0040 TetR promoter||MG1655||Amp
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·I20270 Promoter J23151 Construct (in TOP10)<br\>
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|-
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|align=left|BY4741 yeast culture||||
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7/8/08<br\>
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|-
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·S17-1 + RP4<br\>
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|valign=top rowspan=4|7/1/08
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·R0010 Lac promoter w/CAP binding site (in MG strain)<br\>
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|align=left|I20260 Promoter J23101 Construct||TOP10||
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·I763004 GFP w/Lac Promoter R0010 (in DH strain)
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|-
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|align=left|I20268 Promoter J23102 Construct||TOP10||
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7/9/08<br>
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|-
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·E0240 GFP generator (brought over from BioCircuit Lab)
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|align=left|I20269 Promoter J23150 Construct||TOP10||
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|-
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7/15/08<br>
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|align=left|I20270 Promoter J23151 Construct||TOP10||
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·pAC88
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|-
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|valign=top rowspan=3|7/8/08
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7/16/08<br>
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|align=left|RP4||S17-1||Amp
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·Elowitz's D29 with KAN resistance, MG1655Z1, vector PCS26 - PAC
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|-
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|align=left|R0010 Lac promoter w/CAP binding site||MG strain||
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7/22/08<br>
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|-
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·DH5alpha RP4
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|align=left|I763004 GFP w/Lac Promoter R0010||DH||
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|-
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7/24/08<br>
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|7/9/08
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·pUC307 (RP1)
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|align=left|E0240 GFP generator (from BioCircuit Lab)||||
 +
|-
 +
|7/15/08
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|align=left|pAC88||||Amp
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|-
 +
|7/16/08
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|align=left|Elowitz's D29 on vector PCS26-PAC||MG1655Z1||Kan
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|-
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|align="center";|7/22/08
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|align=left|RP4||DH5alpha||Amp
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|-
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|7/24/08
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|align=left|pUC307 (RP1)||||Kan
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|-
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|valign=top rowspan=2|8/3/08
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|align=left|P1010(pSB1AC3)||???||Amp+Cam
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|-
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|align=left|P1010(pSB3K3)||???||Kan
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|-
 +
|8/4/08
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|align=left|contain unknown plasmid with Amp, lost KAN||MG1655Z1||Amp
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|-
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|8/7/08
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|align=left|pAC88+pUB307||Top-10||Amp&Kan
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|-
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|8/13/08
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|align=left|BBa_promoterD29 on pSB1AC3||XL1-Blue||Amp+Cam
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|-
 +
|8/26/08
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|align=left|no plasmids(hopefully)||MG1655Z1||none
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|-
 +
|9/3/08
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|align=left|AraC-TetR promoter + E0240 on pSB3K3||XL1-Blue||Kan
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|-
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|valign=top rowspan=2|9/5/08
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|align=left|pAC99||Top-10||Amp
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|-
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|align=left|pAC99+pUB307||Top-10||Amp&Kan
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|-
 +
|valign=top rowspan=3|9/10/08
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|align=left|TrbA||Top-10||Amp
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|-
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|align=left|TrbC||Top-10||Amp
 +
|-
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|align=left|KorA||Top-10||Amp
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|}

Latest revision as of 19:47, 10 September 2008

Home The Team The Project Parts Submitted to the Registry Modeling Notebook Protocols BioBrick Parts Used Glycerol Stocks


DatePartStrainResistance
6/26/08 RP4C600Amp
noneS17-1
6/27/08 R0062 LuxR promoterAmp
R0080 AraC promoterMG1655Amp
R0040 TetR promoterMG1655Amp
BY4741 yeast culture
7/1/08 I20260 Promoter J23101 ConstructTOP10
I20268 Promoter J23102 ConstructTOP10
I20269 Promoter J23150 ConstructTOP10
I20270 Promoter J23151 ConstructTOP10
7/8/08 RP4S17-1Amp
R0010 Lac promoter w/CAP binding siteMG strain
I763004 GFP w/Lac Promoter R0010DH
7/9/08 E0240 GFP generator (from BioCircuit Lab)
7/15/08 pAC88Amp
7/16/08 Elowitz's D29 on vector PCS26-PACMG1655Z1Kan
7/22/08 RP4DH5alphaAmp
7/24/08 pUC307 (RP1)Kan
8/3/08 P1010(pSB1AC3)???Amp+Cam
P1010(pSB3K3)???Kan
8/4/08 contain unknown plasmid with Amp, lost KANMG1655Z1Amp
8/7/08 pAC88+pUB307Top-10Amp&Kan
8/13/08 BBa_promoterD29 on pSB1AC3XL1-BlueAmp+Cam
8/26/08 no plasmids(hopefully)MG1655Z1none
9/3/08 AraC-TetR promoter + E0240 on pSB3K3XL1-BlueKan
9/5/08 pAC99Top-10Amp
pAC99+pUB307Top-10Amp&Kan
9/10/08 TrbATop-10Amp
TrbCTop-10Amp
KorATop-10Amp
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