Edinburgh/2 July 2008

From 2008.igem.org

(Difference between revisions)
 
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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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<head>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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//No need to edit beyond here////////////////////////
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var evtobj=window.event? window.event : e
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this.delayhidemenu(obj2)
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this.delayhidemenu(obj2)
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clearTimeout(this.delayhide)
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if (this.enableiframeshim && typeof this.shimobject!="undefined")
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this.shimobject.style.display='none'
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var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
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init:function(menuid, dselected){
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menuitems[i].parentNode.className+=" selected default"
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}
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</script>
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<style>
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.ddcolortabs{
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padding: 0;
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width: 100%;
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background: transparent;
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voice-family: "\"}\"";
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voice-family: inherit;
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.ddcolortabs ul{
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font: normal 11px Arial, Verdana, sans-serif;
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.ddcolortabs li{
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margin:0 2px 0 0;
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padding:0;
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text-transform:uppercase;
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.ddcolortabs a{
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float:left;
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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padding:0 0 1px 3px;
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text-decoration:none;
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letter-spacing: 1px;
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.ddcolortabs a span{
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float:left;
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display:block;
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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padding: 4px 8px 2px 7px;
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.ddcolortabs a span{
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float:none;
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.ddcolortabs a:hover{
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background-color: #591f20;
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}
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.ddcolortabs a:hover span{
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background-color: #591f20;
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}
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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background-color: #591f20;
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}
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.ddcolortabsline{
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clear: both;
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padding: 0;
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width: 100%;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
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border-bottom-width: 0;
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font:normal 12px Arial;
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line-height:18px;
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z-index:100;
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background-color: white;
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width: 200px;
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visibility: hidden;
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}
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.dropmenudiv_a a{
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width: auto;
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display: block;
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text-indent: 5px;
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border-top: 0 solid #678b3f;
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border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
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padding: 2px 0;
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text-decoration: none;
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color: black;
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}
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* html .dropmenudiv_a a{ /*IE only hack*/
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width: 100%;
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}
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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background-color: #8a3c3d;
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color: white;
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}
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</style>
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</head>
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<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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</ul>
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</div>
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<div class="ddcolortabsline">&nbsp;</div>
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<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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</div>
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<script type="text/javascript">
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//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
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tabdropdown.init("colortab", 3)
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</script>
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</html>
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<table>
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<html>
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<style type="text/css">
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table.calendar          { margin: 0; padding: 2px; }
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table.calendar td      { margin: 0; padding: 1px; vertical-align: top; }
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table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; }
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table.month .dow td    { color:#000000; text-align:center; font-size:100%; }
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table.month td.today    { background-color:#cd0000; }
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table.month td {
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    border: none;
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    margin: 0;
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    padding: 0pt 0.5pt;
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    font-weight: bold;
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    font-size: 9pt;
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    text-align: right;
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    background-color: white;
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    }
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#bodyContent table.month a { background:none; padding:0 }
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.day-active { color:#cd0000 }
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.day-empty  { color:#000000 }
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</style>
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</html>
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{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px"
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</table>
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{| align="left"
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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|}
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:::: '''[[Edinburgh/1_July_2008|< Previous Entry]]'''
:::: '''[[Edinburgh/1_July_2008|< Previous Entry]]'''

Latest revision as of 14:04, 28 August 2008

< Previous Entry

Week 3

Wednesday 2 July 08

  • Digests of M10 and M11 with EcoRI, to determine orientation, were not very clear (Gel 7). Probably simpler just to sequence them, since we will need to check that the ends are intact in any case. Ordered mutagenic primers to remove the EcoRI sites:
    • primer glgcm1f: tgttgaaaaacctgctaaccc
    • primer glgcm1r: aattcgataattttatcgttctc
    • primer glgcm2f: ctcattctgcaacattgattcc
    • primer glgcm2r: ttcacgcgaacgcgcgag


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