Edinburgh/6 August 2008

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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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{| align="left"
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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|}
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:::: '''[[Edinburgh/5_August_2008|< Previous Entry]]'''
:::: '''[[Edinburgh/5_August_2008|< Previous Entry]]'''
Line 332: Line 6:
=== Wednesday 6 August 08 ===
=== Wednesday 6 August 08 ===
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* '''L31''' ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 completed. (OG)
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* Primers have arrived for pCstA. PCR carried out using ''E. coli'' cells as the template ('''P51'''). This was unsuccessful. (CF)
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* Primers have arrived for pCstA. Chris carried out the PCR (that would be '''P51'''). Unsuccessful. (CF)
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* Digestion of M67 (pSB1A2+rbs+''crtB'') with EcoRI/SpeI and M50 (pSB1A2+rbs+''crtI'') with EcoRI/XbaI in preparation for combining the two, with the former as insert and the latter as vector. (CF)
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* Digestion of M67 (pSB1A2+rbs+''crtB'') with EcoRI/SpeI and M50 (pSB1A2+rbs+''crtI'') with EcoRI/XbaI, in preparation for combining the two with the former as insert and the latter as vector. (CF)
+
* Re-digestion of M97-M99 and M101 (all pSB1A2+''cenA'') and M105 and M108 (pSB1A2+''cex'') with a) EcoRI, b) EcoRI/PstI, and M92 (pSB1A2+''cex'') with a) PstI, b) EcoRI/PstI. All run on '''gel 38'''. (Yan)
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** Not enough time to purify/ligate due to high demand for agarose gel following lunchtime. Shall do tomorrow morning at 9. (AM)
+
* Transformation of L30 (Self-ligation of BABEL2+''glgC''-mut1,2,3) to '''plates 89-90''') and L31 (''glgC''-mut1,2 to Edinbrick1) to '''plates 91-92'''. (HX)
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* Re-digestion of M97, M98, M99 and M101 (all pSB1A2+''cenA'') and M105 and M108 (pSB1A2+''cex'') with a) EcoRI, b) EcoRI/PstI, and M92 (pSB1A2+''cex'') with a) PstI, b) EcoRI/PstI. All run on '''gel 38''' (because gel 37 did not look good). (Yan)
+
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* Transformation of L30 (Self-ligation of BABEL2+''glgC''-mut1,2,3) ('''plates 89 and 90''') and L31 (''glgC''-mut1,2 to Edinbrick1) ('''plates 91 and 92''') onto B/W plates. (HX)
+
* Maxipreps '''X6''' (as M50, pSB1A2+rbs+''crtI'') and '''X7''' (as M63: pSB1A2-+rbs+''crtE'') made. (AM)
* Maxipreps '''X6''' (as M50, pSB1A2+rbs+''crtI'') and '''X7''' (as M63: pSB1A2-+rbs+''crtE'') made. (AM)
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* Overnight culture made from plate 93 (pSB1A2+rbs+''crtB'') ready for maxiprep tomorrow. (AM, AH)
+
* Analytical digests using a) EcoRI, b) EcoRI/PstI of M70-M71 (pSB1A2+''glgC''-mut1,2) and M92 (pSB1A2+''cex''). Run on '''gel 39'''. Results do not look good. Bands are wrong size to correspond to vector and no bands match an expected insert size. (CF)
-
* Analytical digests using a) EcoRI, b) EcoRI/PstI of M70, M71 (both pSB1A2+''glgC''-mut1,2) and M92 (pSB1A2
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* Colony on plate 56 (M67, pSB1A2+rbs+''crtB'') subbed to '''plate 93'''. (CF)
-
+''cex''). Run on '''gel 39'''. Results do not look good. Bands are wrong size to correspond to vector and no bands match an expected insert size. (CF)<br />
+
* Overnight culture made from plate 93 (pSB1A2+rbs+''crtB'') ready for maxiprep tomorrow. (AM, AH)<br />
<br />
<br />
:::: '''[[Edinburgh/7_August_2008|Next Entry >]]'''
:::: '''[[Edinburgh/7_August_2008|Next Entry >]]'''

Latest revision as of 14:19, 28 August 2008

< Previous Entry

Week 8

Wednesday 6 August 08

  • Primers have arrived for pCstA. PCR carried out using E. coli cells as the template (P51). This was unsuccessful. (CF)
  • Digestion of M67 (pSB1A2+rbs+crtB) with EcoRI/SpeI and M50 (pSB1A2+rbs+crtI) with EcoRI/XbaI in preparation for combining the two, with the former as insert and the latter as vector. (CF)
  • Re-digestion of M97-M99 and M101 (all pSB1A2+cenA) and M105 and M108 (pSB1A2+cex) with a) EcoRI, b) EcoRI/PstI, and M92 (pSB1A2+cex) with a) PstI, b) EcoRI/PstI. All run on gel 38. (Yan)
  • Transformation of L30 (Self-ligation of BABEL2+glgC-mut1,2,3) to plates 89-90) and L31 (glgC-mut1,2 to Edinbrick1) to plates 91-92. (HX)
  • Maxipreps X6 (as M50, pSB1A2+rbs+crtI) and X7 (as M63: pSB1A2-+rbs+crtE) made. (AM)
  • Analytical digests using a) EcoRI, b) EcoRI/PstI of M70-M71 (pSB1A2+glgC-mut1,2) and M92 (pSB1A2+cex). Run on gel 39. Results do not look good. Bands are wrong size to correspond to vector and no bands match an expected insert size. (CF)
  • Colony on plate 56 (M67, pSB1A2+rbs+crtB) subbed to plate 93. (CF)
  • Overnight culture made from plate 93 (pSB1A2+rbs+crtB) ready for maxiprep tomorrow. (AM, AH)


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