Edinburgh/5 August 2008

From 2008.igem.org

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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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{| align="left"
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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|}
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:::: '''[[Edinburgh/4_August_2008|< Previous Entry]]'''
:::: '''[[Edinburgh/4_August_2008|< Previous Entry]]'''
Line 334: Line 8:
* M72 (pSB1A2+rbs+''dxs''), M79 (BABEL2+''crtE'') and M82 (pSB1A2+rbs+''appY'') submitted for seqencing. (CF)
* M72 (pSB1A2+rbs+''dxs''), M79 (BABEL2+''crtE'') and M82 (pSB1A2+rbs+''appY'') submitted for seqencing. (CF)
* Minipreps '''M97-M104''' of pSB1A2+''cenA'' (plate 85, steaks 2 and 7-13) and '''M105-M108''' of pSB1A2+''cex'' (plate 86) were made. (Yan)
* Minipreps '''M97-M104''' of pSB1A2+''cenA'' (plate 85, steaks 2 and 7-13) and '''M105-M108''' of pSB1A2+''cex'' (plate 86) were made. (Yan)
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* M97-M108 were double-digested with EcoRI/PstI and run on '''gel 37'''. (Yan)
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* M97-M108 were double-digested with EcoRI/PstI and run on '''gel 37'''. The results do not look good except for M97. (Yan)
* Purification and self-ligation of P50 (3rd mutagenesis of ''glgc''-mut1,2) ('''L30'''). (HX)
* Purification and self-ligation of P50 (3rd mutagenesis of ''glgc''-mut1,2) ('''L30'''). (HX)
* M68-M69 (pSB1A2+''glgc''-mut1,2) and M92 (pSB1A2+''cex'') were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)
* M68-M69 (pSB1A2+''glgc''-mut1,2) and M92 (pSB1A2+''cex'') were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)

Latest revision as of 14:19, 28 August 2008

< Previous Entry

Week 8

Tuesday 5 August 08

  • M72 (pSB1A2+rbs+dxs), M79 (BABEL2+crtE) and M82 (pSB1A2+rbs+appY) submitted for seqencing. (CF)
  • Minipreps M97-M104 of pSB1A2+cenA (plate 85, steaks 2 and 7-13) and M105-M108 of pSB1A2+cex (plate 86) were made. (Yan)
  • M97-M108 were double-digested with EcoRI/PstI and run on gel 37. The results do not look good except for M97. (Yan)
  • Purification and self-ligation of P50 (3rd mutagenesis of glgc-mut1,2) (L30). (HX)
  • M68-M69 (pSB1A2+glgc-mut1,2) and M92 (pSB1A2+cex) were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)
  • Digested P36 (glgC-mut1,2) with Edinbrick1 using Spe1/EcoR1 and ligated (L31). (OG)


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