Imperial College/27 August 2008
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+ | {{Imperial/StartPage}}__NOTOC__ | ||
+ | {| cellpadding="10" border="0" | ||
+ | |- valign="top" | ||
+ | |{{#calendar: title=Imperial_College |year=2008 | month=07}} | ||
+ | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
+ | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
+ | | rowspan="2" bgcolor=#ffffff width="100%" | | ||
+ | |} | ||
+ | = 27 August 2008 = | ||
+ | ==Wetlab== | ||
+ | *Today a Midi prep of CAT and the ''B.subtilis'' vectors pDR110 and pDR111 were performed. Validation of the DNA was carried out by running a gel electrophoresis of the DNA. | ||
+ | [[Image:27-8.PNG|center|frame| Lanes : M = Marker, 1 = CAT Midi 1 Digested, 2 =CAT Midi 2 Digested, 3 = pDR110 Midi 1 Undigested, 4 = pDR110 Midi 2 Undigested, 5 = pDR111 Midi 1 Undigested, 6 = pDR111 Midi 2 Undigested]] | ||
+ | |||
+ | * Both the plasmids and the CAT insert appear to be the correct size and contamination appears to be fairly negligible | ||
+ | |||
==Dry Lab== | ==Dry Lab== | ||
*Obtained a second better quality microscope video of B. Subtilis swimming (better contrast, better focus...) | *Obtained a second better quality microscope video of B. Subtilis swimming (better contrast, better focus...) | ||
*Failed to track the cells using Spot Tracker plugin in ImageJ. More investigation needed. | *Failed to track the cells using Spot Tracker plugin in ImageJ. More investigation needed. | ||
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:40, 28 October 2008
27 August 2008Wetlab
Dry Lab
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