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Purdue/18 September 2008
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| + | Digested SOS plasmids: | ||
| + | *Mixture: | ||
| + | **40uL DNA | ||
| + | **5uL NEB Buffer 2 (10x) | ||
| + | **13uL ddH2O | ||
| + | **0.5uL BSA | ||
| + | **1uL EcoRI | ||
| + | **1uL SpeI | ||
| + | *Mixed, stored at 37C for 1 hour | ||
| + | *Placed in -20C to halt reaction | ||
| + | |||
| + | We'll run it through a gel and purify the SOS and LacZ parts soon. Then ligation, transformation, testing! | ||
| + | |||
| + | |||
| + | '''Edited by Janie Stine''' | ||
Latest revision as of 15:05, 18 September 2008
Click Here to return to the notebook.
Digested SOS plasmids:
- Mixture:
- 40uL DNA
- 5uL NEB Buffer 2 (10x)
- 13uL ddH2O
- 0.5uL BSA
- 1uL EcoRI
- 1uL SpeI
- Mixed, stored at 37C for 1 hour
- Placed in -20C to halt reaction
We'll run it through a gel and purify the SOS and LacZ parts soon. Then ligation, transformation, testing!
Edited by Janie Stine
