Template:Team:UC Berkeley/Notebook/AL construction

From 2008.igem.org

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--[[User:Aronlau|Aronlau]] 01:15, 5 June 2008 (UTC)
+
==pBca1256-K112300==
<pre>
<pre>
PCR al0001/al0002 on pSB3C6-Bjh1501    (508bp, EcoRI/BamHI/DpnI)
PCR al0001/al0002 on pSB3C6-Bjh1501    (508bp, EcoRI/BamHI/DpnI)
Line 10: Line 10:
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
</pre>
</pre>
-
 
+
==pBca1256-K112301==
<pre>
<pre>
PCR al0003/al0002 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI)
PCR al0003/al0002 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI)
Line 21: Line 21:
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
</pre>
</pre>
-
 
+
==pBca1256-K112302==
<pre>
<pre>
PCR al0001/al0004 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI)
PCR al0001/al0004 on pSB3C6-Bjh1501    (505bp, EcoRI/BamHI/DpnI)
Line 27: Line 27:
Product is pBca1256-K112302
Product is pBca1256-K112302
-----------------------------------------------
-----------------------------------------------
-
al0001 Forward Biobricking of lambda lysozyme with start codon and no start codon
+
al0001 Forward Biobricking of lambda lysozyme with start codon and no stop codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
-
al0004 Reverse Biobricking of lambda lysozyme with start codon and no start codon
+
al0004 Reverse Biobricking of lambda lysozyme with start codon and no stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc
CGttaGGATCCtacatcaatctctctgaccgttccgc
</pre>
</pre>
-
 
+
==pBca1256-K112303==
<pre>
<pre>
PCR al0003/al0004 on pSB3C6-Bjh1501    (502bp, EcoRI/BamHI/DpnI)
PCR al0003/al0004 on pSB3C6-Bjh1501    (502bp, EcoRI/BamHI/DpnI)
Line 43: Line 43:
CGttaGGATCCtacatcaatctctctgaccgttccgc
CGttaGGATCCtacatcaatctctctgaccgttccgc
</pre>
</pre>
-
 
+
==pBca1256-K112304==
<pre>
<pre>
PCR al0005/al0002 on pSB3C6-Bjh1501    (512bp, EcoRI/BamHI/DpnI)
PCR al0005/al0002 on pSB3C6-Bjh1501    (512bp, EcoRI/BamHI/DpnI)
Line 54: Line 54:
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
</pre>
</pre>
-
 
+
==pBca1256-K112305==
<pre>
<pre>
PCR al0006/al0002 on pSB3C6-Bjh1501    (527bp, EcoRI/BamHI/DpnI)
PCR al0006/al0002 on pSB3C6-Bjh1501    (527bp, EcoRI/BamHI/DpnI)
Line 65: Line 65:
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
</pre>
</pre>
-
 
+
==pBca1256-K112306==
<pre>
<pre>
PCR al0007/al0008 on pSB3C6-Bjh1502    (349bp, EcoRI/BamHI/DpnI)
PCR al0007/al0008 on pSB3C6-Bjh1502    (349bp, EcoRI/BamHI/DpnI)
Line 71: Line 71:
Product is pBca1256-K112306
Product is pBca1256-K112306
-----------------------------------------------
-----------------------------------------------
-
al0007 Forward Biobricking of lambda holin with start and stop codon
+
al0007 Forward Biobricking of lambda holin with start and stop codons
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
-
al0008 Reverse Biobricking of lambda holin with start and stop codon
+
al0008 Reverse Biobricking of lambda holin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112307==
<pre>
<pre>
PCR al0009/al0008 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI)
PCR al0009/al0008 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI)
Line 87: Line 87:
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112308==
<pre>
<pre>
PCR al0007/al0010 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI)
PCR al0007/al0010 on pSB3C6-Bjh1502    (346bp, EcoRI/BamHI/DpnI)
Line 98: Line 98:
CGttaGGATCCttgatttctaccatcttctactccggc
CGttaGGATCCttgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112309==
<pre>
<pre>
PCR al0009/al0010 on pSB3C6-Bjh1502    (343bp, EcoRI/BamHI/DpnI)
PCR al0009/al0010 on pSB3C6-Bjh1502    (343bp, EcoRI/BamHI/DpnI)
Line 109: Line 109:
CGttaGGATCCttgatttctaccatcttctactccggc
CGttaGGATCCttgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112310==
<pre>
<pre>
PCR al0011/al0008 on pSB3C6-Bjh1502    (353bp, EcoRI/BamHI/DpnI)
PCR al0011/al0008 on pSB3C6-Bjh1502    (353bp, EcoRI/BamHI/DpnI)
Line 120: Line 120:
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112311==
<pre>
<pre>
PCR al0012/al0008 on pSB3C6-Bjh1502    (369bp, EcoRI/BamHI/DpnI)
PCR al0012/al0008 on pSB3C6-Bjh1502    (369bp, EcoRI/BamHI/DpnI)
Line 127: Line 127:
-----------------------------------------------
-----------------------------------------------
al0012 Forward Biobricking of lambda holin with rbs site
al0012 Forward Biobricking of lambda holin with rbs site
-
CCATAGAATTCatgAGATCTattgggggtaagacCtgaagatgccag
+
CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
al0008 Reverse Biobricking of lambda holin with rbs site
al0008 Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112312==
<pre>
<pre>
-
PCR al0013/al0014 on pSB3C6-Bjh1503    (355bp, EcoRI/BamHI/DpnI)
+
PCR al0013/al0008 on pSB3C6-Bjh1503    (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
Product is pBca1256-K112312
Line 139: Line 139:
al0013 Forward Biobricking of lambda antiholin with start and stop codons
al0013 Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
-
al0014 Reverse Biobricking of lambda antiholin with start and stop codons
+
al0008 Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112313==
<pre>
<pre>
-
PCR al0015/al0014 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI)
+
PCR al0014/al0008 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
Product is pBca1256-K112313
-----------------------------------------------
-----------------------------------------------
-
al0015 Forward Biobricking of lambda antiholin with stop codon and no start codon
+
al0014 Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
-
al0014 Reverse Biobricking of lambda antiholin with stop codon and no start codon
+
al0008 Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112314==
<pre>
<pre>
-
PCR al0013/al0016 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI)
+
PCR al0013/al0010 on pSB3C6-Bjh1503    (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
Product is pBca1256-K112314
Line 161: Line 161:
al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon
al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
-
al0016 Reverse Biobricking of lambda antiholin with start codon and no stop codon
+
al0010 Reverse Biobricking of lambda antiholin with start codon and no stop codon
-
CGttaGGATCCttgatttctaccatcttctactccgg
+
CGttaGGATCCttgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112315==
<pre>
<pre>
-
PCR al0015/al0016 on pSB3C6-Bjh1503    (349bp, EcoRI/BamHI/DpnI)
+
PCR al0014/al0010 on pSB3C6-Bjh1503    (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
Product is pBca1256-K112315
-----------------------------------------------
-----------------------------------------------
-
al0015 Forward Biobricking of lambda antiholin with no start and stop codons
+
al0014 Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
-
al0016 Reverse Biobricking of lambda antiholin with no start and stop codons
+
al0010 Reverse Biobricking of lambda antiholin with no start and stop codons
-
CGttaGGATCCttgatttctaccatcttctactccgg
+
CGttaGGATCCttgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112316==
<pre>
<pre>
-
PCR al0017/al0014 on pSB3C6-Bjh1503    (359bp, EcoRI/BamHI/DpnI)
+
PCR al0015/al0008 on pSB3C6-Bjh1503    (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
Product is pBca1256-K112316
-----------------------------------------------
-----------------------------------------------
-
al0017 Forward Biobricking of lambda antiholin with spacer
+
al0015 Forward Biobricking of lambda antiholin with spacer
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
-
al0014 Reverse Biobricking of lambda antiholin with spacer
+
al0008 Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
</pre>
</pre>
-
 
+
==pBca1256-K112317==
<pre>
<pre>
-
PCR al0018/al0014 on pSB3C6-Bjh1503    (375bp, EcoRI/BamHI/DpnI)
+
PCR al0016/al0008 on pSB3C6-Bjh1503    (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
Product is pBca1256-K112317
-----------------------------------------------
-----------------------------------------------
-
al0018 Forward Biobricking of lambda antiholin with rbs site
+
al0016 Forward Biobricking of lambda antiholin with rbs site
-
CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc
+
CCATAGAATTCatgAGATCTccccttattgggggtaagacatg
-
al0014 Reverse Biobricking of lambda antiholin with rbs site
+
al0008 Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc
CGttaGGATCCttattgatttctaccatcttctactccggc
 +
</pre>
 +
==pBca1256-K112318==
 +
<pre>
 +
PCR al0017/al0018 on MG1655    (467bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112318
 +
-----------------------------------------------
 +
al0017 Forward Biobricking of bolA promoter
 +
CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc
 +
al0018 Reverse Biobricking of bolA promoter
 +
CGttaGGATCCcttctatccgctcacgtatc
 +
</pre>
 +
 +
==pBca1256-K112319==
 +
<pre>
 +
PCR al0019/al0024 on MG1655 (404bp, gp = A)
 +
PCR al0023/al0023 on MG1655 (86bp, gp=B)
 +
-----------------------------------------------
 +
PCR al0019/al0020 on A+B    (465bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112319
 +
-----------------------------------------------
 +
al0019 Forward Biobricking of ftsQ promoter
 +
CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg
 +
al0020 Reverse Biobricking of ftsQ promoter
 +
CGttaGGATCCcctcttcttcgctgtttcgc
 +
al0023 Forward Removing of EcoRI
 +
ggtagtacgaattGtggaactggcg
 +
al0024 Reverse Removing of EcoRI
 +
cgccagttccaCaattcgtactacc
 +
</pre>
 +
 +
==pBca1256-K112320==
 +
<pre>
 +
PCR al0021/al0022 on MG1655    (804bp, EcoRI/BamHI/DpnI)
 +
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112320
 +
-----------------------------------------------
 +
al0021 Forward Biobricking of ftsAZ promoter
 +
CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg
 +
al0022 Reverse Biobricking of ftsAZ promoter
 +
CGttaGGATCCgtaccaatctccagtcctactaccag
 +
</pre>
 +
 +
==pBjh1601AC-K112321==
 +
<pre>
 +
PCR JH088F/JHO88R on Salmonella L2.    (435bp, EcoRI/BamHI/DpnI)
 +
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
 +
Product is pBjh1601AC-K112321
 +
-----------------------------------------------
 +
JH088F  Forward biobricking of {H-NS!} 
 +
cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
 +
JH088R  Reverse biobricking of {H-NS!} 
 +
cgttaGGATCCTTATTGCTTGATCAGGAAATCG
 +
</pre>
 +
 +
==pBjh1601CK-K112322==
 +
<pre>
 +
PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBjh1601CK            (EcoRI/BamHI, 3134+910, L)
 +
Product is pBjh1601CK-K112322
 +
-----------------------------------------------
 +
JH089F  Forward biobricking of {Pdps} 
 +
cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc
 +
JH089R  Reverse biobricking of {Pdps} 
 +
cgttaGGATCCaatctcatatcctcttgatg
 +
</pre>
 +
 +
==pBCa1601AC-K112323==
 +
<pre>
 +
PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
 +
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
 +
Product is pBjh1601AC-K112323
 +
-----------------------------------------------
 +
ca998  Forward biobricking of {H-NS!} Salmonella 
 +
gtatcacgaggcagaatttcag
 +
JH090R  Reverse biobricking of {H-NS!} Salmonella 
 +
cgttaGGATCCTTATTcCTTGATCAGG
 +
</pre>
 +
==pBCa1601CK-K112324==
 +
<pre>
 +
Digest (BgIII methylated) K112902 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112324
 +
</pre>
 +
 +
==pBCa1601CK-K112325==
 +
<pre>
 +
Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112325
 +
</pre>
 +
 +
==pBCa1601AC-K112327==
 +
<pre>
 +
Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AC-K112327
 +
</pre>
 +
 +
==pBCa1601AC-K112328==
 +
<pre>
 +
Digest (BgIII methylated) K112227 AK, (BamHI methylated) K112102 KC      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AC-K112328
 +
</pre>
 +
 +
==pBCa1601AC-K112329==
 +
<pre>
 +
Digest  (BgIII methylated) K112230 AK, (BamHI methylated) K112113 CK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AC-K112329
 +
</pre>
 +
 +
==pBCa1601AC-K112330==
 +
<pre>
 +
Digest K112614 AK (BgIII, XhoI)
 +
Digest K112105 KC(BamHI, XhoI)
 +
Product is pBjh1601AC-K112330
 +
</pre>
 +
 +
==pBCa1601AC-K112331==
 +
<pre>
 +
Digest K112615 AK (BgIII, XhoI)
 +
Digest K112108 KC(BamHI, XhoI)
 +
Product is pBjh1601AC-K112331
 +
</pre>
 +
 +
==pBCa1601AC-K112332==
 +
<pre>
 +
Digest  (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AC-K112332
 +
</pre>
 +
 +
==pBCa1601AC-K112333==
 +
<pre>
 +
Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AC-K112333
 +
</pre>
 +
 +
==pBca1601AK-K112334==
 +
<pre>
 +
Digest (BgIII methylated) K112230 AC, (BamHI methylated) K112324 CK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112334
 +
</pre>
 +
 +
==pBca1601AK-K112335==
 +
<pre>
 +
Digest (BgIII methylated) K112227 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112335
 +
</pre>
 +
 +
==pBca1601AK-K112336==
 +
<pre>
 +
Digest (BgIII methylated) K112614 AC   (BamHI/XhoI)
 +
Digest (BamHI methylated) K112324 CK      (BgIII/XHoI)
 +
Product is pBjh1601AK-K112336
 +
</pre>
 +
 +
==pBca1601AK-K112337==
 +
<pre>
 +
Digest (BgIII methylated) K112615 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112337
 +
</pre>
 +
 +
==pBca1601AK-K112339==
 +
<pre>
 +
Digest (BgIII methylated) K112327 AC    (BamHI/XhoI)
 +
Digest (BamHI methylated) K112324 CK    (BgIII/XHoI)
 +
Product is pBjh1601AK-K112339
 +
</pre>
 +
 +
 +
==pBca1601AK-K112340==
 +
<pre>
 +
Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112340
 +
</pre>
 +
 +
==pBca1601AK-K112341==
 +
<pre>
 +
Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112341
 +
</pre>
 +
 +
==pBca1601AK-K112342==
 +
<pre>
 +
Digest (BgIII methylated) K112330 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112342
 +
</pre>
 +
 +
 +
==pBca1601AK-K11234==
 +
<pre>
 +
Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112344
 +
</pre>
 +
 +
==pBca1601AK-K112348==
 +
<pre>
 +
Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112348
 +
</pre>
 +
 +
==pBca1601AK-K112349==
 +
<pre>
 +
Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601AK-K112349
 +
</pre>
 +
 +
==pBca1601AK-K112350==
 +
<pre>
 +
Digest (BgIII methylated) K112330 AC (BamHI/XhoI)
 +
Digest (BamHI methylated) K112325 CK    (BgIII/XHoI)
 +
Product is pBjh1601AK-K112350
 +
</pre>
 +
==pBca1601AK-K112351==
 +
<pre>
 +
Digest (BgIII methylated) K112331 AC (BamHI/XhoI)
 +
Digest (BamHI methylated) K112325 CK    (BgIII/XHoI)
 +
Product is pBjh1601AK-K112351
 +
</pre>
 +
==pBca1601CK-K112354==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112334 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112354
 +
</pre>
 +
==pBca1601CK-K112355==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112335 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112354
 +
</pre>
 +
==pBca1601CK-K112356==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112336 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112354
 +
</pre>
 +
==pBca1601CK-K112357==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112337 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112354
 +
</pre>
 +
==pBca1601CK-K112360==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112340 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112360
 +
</pre>
 +
==pBca1601CK-K112361==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112341 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112361
 +
</pre>
 +
==pBca1601CK-K112362==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112342 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112362
 +
</pre>
 +
==pBca1601CK-K112363==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112343 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112363
 +
</pre>
 +
==pBca1601CK-K112368==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112368
 +
</pre>
 +
==pBca1601CK-K112369==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112349 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112369
 +
</pre>
 +
==pBca1601CK-K112370==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112350 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112370
 +
</pre>
 +
==pBca1601CK-K112371==
 +
<pre>
 +
Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112371
 +
</pre>
 +
==pBca1601CK-K112372==
 +
<pre>
 +
Digest (BgIII methylated) K112121 CA, (BamHI methylated) K112709 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112372
 +
</pre>
 +
==pBca1601CK-K112373==
 +
<pre>
 +
Digest (BgIII methylated) BBa_K112232 CA, (BamHI methylated) K112709 AK    (BamHI/BgIII/XHoI)
 +
Product is pBjh1601CK-K112373
</pre>
</pre>

Latest revision as of 02:10, 29 October 2008

Contents

pBca1256-K112300

PCR al0001/al0002 on pSB3C6-Bjh1501     (508bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112300
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start and stop codons
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with start and stop codons
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112301

PCR al0003/al0002 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112301
-----------------------------------------------
al0003	Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112302

PCR al0001/al0004 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112302
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start codon and no stop codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with start codon and no stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112303

PCR al0003/al0004 on pSB3C6-Bjh1501     (502bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112303
-----------------------------------------------
al0003	Forward Biobricking of lambda lysozyme with no start/stop codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with no start/stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112304

PCR al0005/al0002 on pSB3C6-Bjh1501     (512bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112304
-----------------------------------------------
al0005	Forward Biobricking of lambda lysozyme with spacer
CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with spacer
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112305

PCR al0006/al0002 on pSB3C6-Bjh1501     (527bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112305
-----------------------------------------------
al0006	Forward Biobricking of lambda lysozyme with rbs site
CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
al0002	Reverse Biobricking of lambda lysozyme with rbs site
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112306

PCR al0007/al0008 on pSB3C6-Bjh1502     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112306
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and stop codons
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0008	Reverse Biobricking of lambda holin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112307

PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0008	Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112308

PCR al0007/al0010 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0010	Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112309

PCR al0009/al0010 on pSB3C6-Bjh1502     (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0010	Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112310

PCR al0011/al0008 on pSB3C6-Bjh1502     (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
-----------------------------------------------
al0011	Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112311

PCR al0012/al0008 on pSB3C6-Bjh1502     (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311
-----------------------------------------------
al0012	Forward Biobricking of lambda holin with rbs site
CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
al0008	Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112312

PCR al0013/al0008 on pSB3C6-Bjh1503     (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112313

PCR al0014/al0008 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112314

PCR al0013/al0010 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0010	Reverse Biobricking of lambda antiholin with start codon and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112315

PCR al0014/al0010 on pSB3C6-Bjh1503     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0010	Reverse Biobricking of lambda antiholin with no start and stop codons
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112316

PCR al0015/al0008 on pSB3C6-Bjh1503     (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
-----------------------------------------------
al0015	Forward Biobricking of lambda antiholin with spacer
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112317

PCR al0016/al0008 on pSB3C6-Bjh1503     (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
-----------------------------------------------
al0016	Forward Biobricking of lambda antiholin with rbs site
CCATAGAATTCatgAGATCTccccttattgggggtaagacatg
al0008	Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112318

PCR al0017/al0018 on MG1655     (467bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112318
-----------------------------------------------
al0017	Forward Biobricking of bolA promoter
CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc
al0018	Reverse Biobricking of bolA promoter
CGttaGGATCCcttctatccgctcacgtatc

pBca1256-K112319

PCR al0019/al0024 on MG1655 (404bp, gp = A)
PCR al0023/al0023 on MG1655 (86bp, gp=B)
-----------------------------------------------
PCR al0019/al0020 on A+B     (465bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112319
-----------------------------------------------
al0019	Forward Biobricking of ftsQ promoter
CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg
al0020	Reverse Biobricking of ftsQ promoter
CGttaGGATCCcctcttcttcgctgtttcgc
al0023 Forward Removing of EcoRI
ggtagtacgaattGtggaactggcg
al0024 Reverse Removing of EcoRI
cgccagttccaCaattcgtactacc

pBca1256-K112320

PCR al0021/al0022 on MG1655     (804bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112320
-----------------------------------------------
al0021	Forward Biobricking of ftsAZ promoter
CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg
al0022	Reverse Biobricking of ftsAZ promoter
CGttaGGATCCgtaccaatctccagtcctactaccag

pBjh1601AC-K112321

PCR JH088F/JHO88R on Salmonella L2.     (435bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112321
-----------------------------------------------
JH088F   Forward biobricking of {H-NS!}   
cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
JH088R   Reverse biobricking of {H-NS!}   
cgttaGGATCCTTATTGCTTGATCAGGAAATCG

pBjh1601CK-K112322

PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601CK             (EcoRI/BamHI, 3134+910, L)
Product is pBjh1601CK-K112322
-----------------------------------------------
JH089F   Forward biobricking of {Pdps}   
cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc
JH089R   Reverse biobricking of {Pdps}   
cgttaGGATCCaatctcatatcctcttgatg

pBCa1601AC-K112323

PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112323
-----------------------------------------------
ca998   Forward biobricking of {H-NS!} Salmonella   
gtatcacgaggcagaatttcag
JH090R   Reverse biobricking of {H-NS!} Salmonella   
cgttaGGATCCTTATTcCTTGATCAGG

pBCa1601CK-K112324

Digest (BgIII methylated) K112902 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112324

pBCa1601CK-K112325

Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112325

pBCa1601AC-K112327

Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112327

pBCa1601AC-K112328

Digest (BgIII methylated) K112227 AK, (BamHI methylated) K112102 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112328

pBCa1601AC-K112329

Digest  (BgIII methylated) K112230 AK, (BamHI methylated) K112113 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112329

pBCa1601AC-K112330

Digest K112614 AK (BgIII, XhoI)
Digest K112105 KC(BamHI, XhoI)
Product is pBjh1601AC-K112330

pBCa1601AC-K112331

Digest K112615 AK (BgIII, XhoI)
Digest K112108 KC(BamHI, XhoI)
Product is pBjh1601AC-K112331

pBCa1601AC-K112332

Digest  (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112332

pBCa1601AC-K112333

Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112333

pBca1601AK-K112334

Digest (BgIII methylated) K112230 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112334

pBca1601AK-K112335

Digest (BgIII methylated) K112227 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112335

pBca1601AK-K112336

Digest (BgIII methylated) K112614 AC	  (BamHI/XhoI)
Digest (BamHI methylated) K112324 CK      (BgIII/XHoI)
Product is pBjh1601AK-K112336

pBca1601AK-K112337

Digest (BgIII methylated) K112615 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112337

pBca1601AK-K112339

Digest (BgIII methylated) K112327 AC     (BamHI/XhoI)
Digest (BamHI methylated) K112324 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112339


pBca1601AK-K112340

Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112340

pBca1601AK-K112341

Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112341

pBca1601AK-K112342

Digest (BgIII methylated) K112330 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112342


pBca1601AK-K11234

Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112344

pBca1601AK-K112348

Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112348

pBca1601AK-K112349

Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112349

pBca1601AK-K112350

Digest (BgIII methylated) K112330 AC	 (BamHI/XhoI)
Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112350

pBca1601AK-K112351

Digest (BgIII methylated) K112331 AC	 (BamHI/XhoI)
Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112351

pBca1601CK-K112354

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112334 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112355

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112335 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112356

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112336 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112357

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112337 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112360

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112340 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112360

pBca1601CK-K112361

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112341 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112361

pBca1601CK-K112362

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112342 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112362

pBca1601CK-K112363

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112343 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112363

pBca1601CK-K112368

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112368

pBca1601CK-K112369

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112349 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112369

pBca1601CK-K112370

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112350 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112370

pBca1601CK-K112371

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112371

pBca1601CK-K112372

Digest (BgIII methylated) K112121 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112372

pBca1601CK-K112373

Digest (BgIII methylated) BBa_K112232 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112373
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