Team:Caltech/Protocols/H2O2 Production Assay

From 2008.igem.org

(Difference between revisions)
(New page: {{Caltech_iGEM_08| Content= <div style="font-size:18pt;"> <font face="verdana" style="color:#BB4400">Measuring H<sub>2</sub>O<sub>2</sub> Concentration</font></div> __NOTOC__ <br> Insert...)
 
(5 intermediate revisions not shown)
Line 3: Line 3:
<div style="font-size:18pt;">
<div style="font-size:18pt;">
-
<font face="verdana" style="color:#BB4400">Measuring H<sub>2</sub>O<sub>2</sub> Concentration</font></div>
+
<font face="verdana" style="color:#CC3300">H<sub>2</sub>O<sub>2</sub> Production Assay</font></div>
__NOTOC__
__NOTOC__
<br>
<br>
-
Insert protocol here
+
# Grow an overnight culture of JI377 transformed with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K137076 K137076] or [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2622 luxR] (negative control) on pSB1A2 in SOC + Amp.
 +
# In the morning, dilute culture 1:100 into 5 ml SOC +Amp +IPTG in triplicate.
 +
# Grow cultures to an OD600 of ~0.8, then [[Team:Caltech/Protocols/Measuring_H2O2|assay supernatant]] for hydrogen peroxide approximately every 30 minutes.
 +
# 1hr after reaching an OD600 of ~0.8, induce cultures with 10 nM AHL.
}}
}}

Latest revision as of 00:13, 30 October 2008


iGEM 2008



Home

People

Project Details

Protocols

Completed Systems

Biosafety

Support


H2O2 Production Assay


  1. Grow an overnight culture of JI377 transformed with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K137076 K137076] or [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2622 luxR] (negative control) on pSB1A2 in SOC + Amp.
  2. In the morning, dilute culture 1:100 into 5 ml SOC +Amp +IPTG in triplicate.
  3. Grow cultures to an OD600 of ~0.8, then assay supernatant for hydrogen peroxide approximately every 30 minutes.
  4. 1hr after reaching an OD600 of ~0.8, induce cultures with 10 nM AHL.
Caltech logo.gif
Caltech footer.jpg
Retrieved from "http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay"