Alberta NINT/16 May 2008
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+ | lab work (SD, JD): | ||
+ | |||
+ | PCR of T/A I oligonucleotide using Term + and Term - primers | ||
+ | DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for | ||
+ | insertion into plasmid. | ||
+ | Ran on 2% agarose gel. | ||
+ | [[Image:UVP00296annotated.jpg]] |
Latest revision as of 21:40, 11 October 2008
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lab work (SD, JD):
PCR of T/A I oligonucleotide using Term + and Term - primers DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion into plasmid. Ran on 2% agarose gel.